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Molecular cloning and characterization of the novel lection gene. Primers were designed based on conserved sequences of other lectin genes from Amaryllidaceae. A novel lectin gene was isolated from Zephyrathes grandiflora. The full-length cDNA of Zephyrathes grandiflora lectin was 955bp, containing an open reading frame (576bp) encoding a peptide of 191 amino acids. The gene was named as zga (Zephyrathes grandiflora agglutinin), with its encoding protein showing 59% similarity to GNA.

基因克隆及序列分析：根据石蒜科植物凝集素的保守区段，设计引物，采用RACE技术从韭莲中克隆出长度为952bp的新凝集素基因，包含了完整的编码序列(576bp)，它编码一个全长191个氨基酸的蛋白，与雪花莲外源凝集的相似性达59％，被命名为ZGA(Zephyrathes grandiflora agglutinin)。

The main findings of the study are as follows:(1) Both N2 and P3 are the characteristic ERPs under nogo inhibition,but P3 amplitude is a good indicator of inhibition processing depth;(2) Through ERP waveforms separation from encoding of stimuli sequence,we verify that the capacity of visual working memory is about 4 units;(3) In the go/nogo sequence response, encoding is mainly the function of right hemisphere, but inhibition retrieval is concentrated on left frontal area and anterior cingulated cortex.

本研究获得了以下结论：(1)在nogo抑制条件下，N2和P3波是特征的抑制波，但P3的波幅是反应抑制加工深度的良好指标；(2)对刺激的顺序编码加工的ERP验证了工作记忆的处理单元的容量为4左右；(3)在Go/nogo序列反应中，编码加工体现为右脑优势，而抑制提取的加工负波集中在左脑前额和前角回区。

Analysis of Elaphurus davidianus's sequences of mitochondrial cytb Amino acids,measured its Encoding box contains 1,140 nucleotides encoding 379 amino acids.

对麋鹿cytb基因的氨基酸序列进行分析，测得麋鹿线粒体cytb基因编码框包含1140个核苷编码379个氨基酸。

Result: Compared to normal subjects, some functional regions associated with encoding, maintenance and retrieval process showed increased activation in schizophrenia patients, i.e. right precuneus for encoding process, left PMA, left DLPFC, right precuneus and left VLPFC for maintaince process, left PMA for retrieval process. In addition, subcortical structures, primary motor cortex and some verbal regions in left temporal lobe also showed more activation.

结果：与正常组比较，患者组工作记忆不同认知过程的执行脑区激活程度增加，编码期激活增加脑区为右侧楔前叶，维持期激活增加脑区为左侧PMA、左侧DLPFC、右侧楔前叶及左侧VLPFC，提取期激活增加脑区为左侧PMA，而且患者组还激活了更多皮层下结构、初级运动皮层及左侧颞叶语言相关脑区。

The cDNA (BplPAL1) encoding phenylalanine ammonia-lyase were cloned from Betula platyphylla by reverse transcription polymerase chain reaction and 5' and 3' rapid amplification of cDNA ends, which contains an open reading frame (2322 bp) encoding 773 amino acids. The amino acid sequences contain two functional domains of PAL-HAL and PAL, and the sequence of enzyme active site, which has 60%~73% of consistency with other five plant species and the consistency with Rhizophora mangle is the highest (73.1%).

利用RT-PCR和RACE技术从白桦中克隆了编码苯丙氨酸解氨酶的cDNA，其2322bp的ORF编码773个氨基酸，其推导的氨基酸序列包含PAL-HAL和PAL2个功能域以及酶活性中心序列GTITASGDLVPLSYIA，该序列同其它5种植物的序列一致性为60%~73%，其中与美洲红树最高为73.1%。

The margin of the white matter tract become more clearly with the increasing of the number of gradient encoding directions, especially the detail of the brain stem. But the measured FA of the centrum semiovale, genu and splenium of corpous callosum, the internal capsule, thalamus and the pons with 6, 13 and 21 diffusion gradient encoding directions showed no statically difference.

随着弥散梯度编码方向的增加，FA图质量提高，对白质纤维束细节的显示也更清楚，尤其是对脑干结构的分辨，但成像时间延长；3种不同弥散梯度编码方向的DTI扫描方案所观察到的半卵圆中心、胼胝体膝部、胼胝体压部、内囊、丘脑及桥脑的FA值不存在统计学显著性差异。

Results of sequence and homology analysis show that MD 1 has 97% similarity with mark in maize chloroplast genome,a gene encoding RNA maturase involved in group Ⅱ intron splicing of RNA transcript;MD2 has 99% similarity with the gene serine/threonine phosphorylase 2C in Sporobolus stapfianus;and MD3 has 99% similarity with rice the gene encoding metacaspase,an arginine/lysine-specific cysteine protease.

序列分析和同源性比对表明，MD1与编码成熟酶的玉米叶绿体基因matK有97%的相似性，MD2与极端耐旱植物Sporobolus stapfianus编码丝氨酸/苏氨酸蛋白磷酸酶的PP2C基因有99%的相似性，MD3与属精氨酸/赖氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase酶基因有99%的相似性。根据MD2片段序列，结合电子克隆和RT-PCR方法，克隆出一条1731 bp的全长cDNA序列，它编码388个氨基酸。

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna，利用rt pcr方法，先克隆出一包括hccr 2编码区的长约1 kb的片段，并将其与t载体连接并转化，测序证实无误后，以hccr 2/t载体为模板，再通过pcr克隆出hccr 2的编码区序列，将其连接到pires2 egfp真核表达载体，并通过测序获得证实。

METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing.

从人肝癌细胞株HepG2提取RNA，利用RTPCR方法，先克隆出一包括HCCR2编码区的长约1 kb的片段，并将其与T载体连接并转化，测序证实无误后，以HCCR2/T载体为模板，再通过PCR克隆出HCCR2的编码区序列，将其连接到pIRES2EGFP真核表达载体，并通过测序获得证实。

It is caused by mutations in dyskerin-encoding genes, telomerase-encoding RNA component genes and reverse transcriptase genes, as well as other uncharacterized genes. There are three inherited forms, including X-linked, autosomal dominant and autosomal recessive inheritance.

先天性角化不良是与编码角化不良蛋白基因、编码端粒酶的RNA组份基因、编码端粒酶的逆转录酶基因突变及其他未确认的致病基因突变引起的基因病，其遗传方式有：X-性联隐性遗传、常染色体显性遗传及常染色体隐性遗传。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。