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Result: Compared to normal subjects, some functional regions associated with encoding, maintenance and retrieval process showed increased activation in schizophrenia patients, i.e. right precuneus for encoding process, left PMA, left DLPFC, right precuneus and left VLPFC for maintaince process, left PMA for retrieval process. In addition, subcortical structures, primary motor cortex and some verbal regions in left temporal lobe also showed more activation.

结果:与正常组比较,患者组工作记忆不同认知过程的执行脑区激活程度增加,编码期激活增加脑区为右侧楔前叶,维持期激活增加脑区为左侧PMA、左侧DLPFC、右侧楔前叶及左侧VLPFC,提取期激活增加脑区为左侧PMA,而且患者组还激活了更多皮层下结构、初级运动皮层及左侧颞叶语言相关脑区。

The cDNA (BplPAL1) encoding phenylalanine ammonia-lyase were cloned from Betula platyphylla by reverse transcription polymerase chain reaction and 5' and 3' rapid amplification of cDNA ends, which contains an open reading frame (2322 bp) encoding 773 amino acids. The amino acid sequences contain two functional domains of PAL-HAL and PAL, and the sequence of enzyme active site, which has 60%~73% of consistency with other five plant species and the consistency with Rhizophora mangle is the highest (73.1%).

利用RT-PCR和RACE技术从白桦中克隆了编码苯丙氨酸解氨酶的cDNA,其2322bp的ORF编码773个氨基酸,其推导的氨基酸序列包含PAL-HAL和PAL2个功能域以及酶活性中心序列GTITASGDLVPLSYIA,该序列同其它5种植物的序列一致性为60%~73%,其中与美洲红树最高为73.1%。

The margin of the white matter tract become more clearly with the increasing of the number of gradient encoding directions, especially the detail of the brain stem. But the measured FA of the centrum semiovale, genu and splenium of corpous callosum, the internal capsule, thalamus and the pons with 6, 13 and 21 diffusion gradient encoding directions showed no statically difference.

随着弥散梯度编码方向的增加,FA图质量提高,对白质纤维束细节的显示也更清楚,尤其是对脑干结构的分辨,但成像时间延长;3种不同弥散梯度编码方向的DTI扫描方案所观察到的半卵圆中心、胼胝体膝部、胼胝体压部、内囊、丘脑及桥脑的FA值不存在统计学显著性差异。

Results of sequence and homology analysis show that MD 1 has 97% similarity with mark in maize chloroplast genome,a gene encoding RNA maturase involved in group Ⅱ intron splicing of RNA transcript;MD2 has 99% similarity with the gene serine/threonine phosphorylase 2C in Sporobolus stapfianus;and MD3 has 99% similarity with rice the gene encoding metacaspase,an arginine/lysine-specific cysteine protease.

序列分析和同源性比对表明,MD1与编码成熟酶的玉米叶绿体基因matK有97%的相似性,MD2与极端耐旱植物Sporobolus stapfianus编码丝氨酸/苏氨酸蛋白磷酸酶的PP2C基因有99%的相似性,MD3与属精氨酸/赖氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase酶基因有99%的相似性。根据MD2片段序列,结合电子克隆和RT-PCR方法,克隆出一条1731 bp的全长cDNA序列,它编码388个氨基酸。

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna,利用rt pcr方法,先克隆出一包括hccr 2编码区的长约1 kb的片段,并将其与t载体连接并转化,测序证实无误后,以hccr 2/t载体为模板,再通过pcr克隆出hccr 2的编码区序列,将其连接到pires2 egfp真核表达载体,并通过测序获得证实。

METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing.

从人肝癌细胞株HepG2提取RNA,利用RTPCR方法,先克隆出一包括HCCR2编码区的长约1 kb的片段,并将其与T载体连接并转化,测序证实无误后,以HCCR2/T载体为模板,再通过PCR克隆出HCCR2的编码区序列,将其连接到pIRES2EGFP真核表达载体,并通过测序获得证实。

It is caused by mutations in dyskerin-encoding genes, telomerase-encoding RNA component genes and reverse transcriptase genes, as well as other uncharacterized genes. There are three inherited forms, including X-linked, autosomal dominant and autosomal recessive inheritance.

先天性角化不良是与编码角化不良蛋白基因、编码端粒酶的RNA组份基因、编码端粒酶的逆转录酶基因突变及其他未确认的致病基因突变引起的基因病,其遗传方式有:X-性联隐性遗传、常染色体显性遗传及常染色体隐性遗传。

Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

近年来研究表明,T NF的两种形式在受体结合、生物学效应等方面各具特征[3,4]。M-TNF通过直接接触在组织局部中发挥效应的特性,有望克服S-TNF在生物治疗应用中引起的远端及全身性毒副作用。

Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

在克隆M-TNF cDNA的基础上,删除两型TNF转换酶酶解部位的编码序列,构建出mTNFm并测序验证;建立只表达膜型T N F的真核细胞株。

A kind of M-TNF recombin ant with a stable,uncleavable mutant typewas constructed for its gene therap y. Methods: On the basis of cloning the M-TNF c D NA encoding region,the authors constructed mTNFm,which could potentially expre ss on the c ell surface with a uncleavable type of TNF resulting in deletion of the sequence encoding enzymatic cleavage site of TNF-α-converting enzyme.

近年来研究表明,T NF的两种形式在受体结合、生物学效应等方面各具特征[3,4]。M-TNF通过直接接触在组织局部中发挥效应的特性,有望克服S-TNF在生物治疗应用中引起的远端及全身性毒副作用。

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