查询词典 destructive activity

Little is known about the influence of minerals and other soil factors on the biocontrol activity of Trichoderma spp.The objectives of this study were to first evaluate the take-all–suppressive activity of T.koningii in field soils representative of the wheat-growing region in the Pacific Northwest of the United States,and then to identify abiotic edaphic parameters that are positively and negatively associated with the biocontrol activity of T.koningii in these soils.Such information will be usefulin selecting sites where T.koningii can be expected to perform optimally;in developing formulations that enhance biocontrol;and perhaps in indicating factors that favor mechanisms involved in the biocontrol activity of this strain such as antibiotic and enzyme biosynthesis.

知之甚少，对矿物和其它土壤因素对生物活性木霉镰刀本研究的目的首先评估全蚀抑制活性t.koningii土壤代表小麦种植区在西北太平洋的美国，然后找出生物土壤参数有积极和消极与生物防治活性t.koningii在soils.such这些信息将有助于在选择地点，可以t.koningii预计进入最佳状态；在发展制剂提高生物防治；或许说明因素，有利于参与机制，在生物防治活动，这株如抗生素和酶的合成。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

The results showed that there was a negative relationship between H2O2 content and CAT activity, thus H2O2 content was low when CAT activity was high. There was a close relationship between H2O2 content and differentiation process. H2O2 could be an important signal transduction factor during bud differentiation. IAA oxidase activity was decreased firstly, and then increased, which affected IAA oxidase and degradation pathway and regulate ratio of cytokinin and auxin. With differentiation process continuing, PPO activity was increased, which could cause browning or vitrifaction of seedling, or even malformation.

研究结果表明，鹅掌楸组培再生过程中，过氧化氢酶活性与H2O2含量变化呈负相关，CAT活性较高时，H2O2含量则较低。H2O2含量与鹅掌楸组培苗分化进程密切相关，H2O2可能作为信号因子在芽分化过程中发挥重要作用；IAA氧化酶活性呈现先下降后增加的变化规律，从而影响IAA氧化降解途径，调控细胞分裂素与生长素含量的比值，影响鹅掌楸芽的再生；PPO活性随培养时间的增加而升高，引起组培苗的褐变或玻璃化，甚至生长畸形。

Soil urease activity, sucrase activity, catalase activity and alkalescent phosphats activity reduced along with the soil layer.

随着农田的风蚀沙化，土壤中粉砂粒含量、有机质和养分含量急剧下降，中砂粒含量趋于增大。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Substitutes on benzal benzene ring of BFP have different effects on ·OH scavenging activity in vitro. 2-Cl,3-Cl,2-CH3,4-CH3 and 3-OCH3 may enhance the activity. 3-CH3 may not change the activity. 2-OCH3 and 4-OCH3 may reduce the activity.

BFP苄基苯环上2-Cl、3-Cl、2-CH3、4-CH3、3-OCH3取代，可增强体外清除·OH活性；3-CH3取代，体外清除·OH活性基本不变；2-OCH3、4-OCH3取代，体外清除·OH活性降低。

For A-early F type MS stars, stellar chromospheric activity decrease with stellar rotation period, but increase with Rossby number; For M type MS stars, stellar chromospheric activity increase with stellar rotation period and Rossby number, and stellar chromospheric activity reach saturation at large color index; For the MS stars between mid-F to M type, stellar chromospheric activity decrease with stellar rotation period and Rossby number.

在第五章中，我们采用不同的磁活动指数研究了晚型恒星磁活动与恒星自转参量(自转周期、Rossby数)、磁活动与恒星色指数以及恒星磁活动指数之间的相关性。大样本分析显示晚型主序恒星的色球活动与恒星自转参量的研究晚型主序恒星的色球活动与恒星自转参量间的关系表现出对恒星光谱型的依赖。

The antimicrobial test and researched was done on wide distillation of different solvents on Terpenoid, polarity separation of ethanol distillable substance, thin layer chromatography of different polarities and distillation of amylase. The results showed that the active substance distilled by 95% ethanol and V methanol: toluene=3:1 had stronger antimicrobial activity on Agaricus blazei Murrill. The antimicrobial activity of ethyl acetate was stronger than wide distilling substance with ethanol, but the water part has not antimicrobial effect. Two straps of ethyl acetate phase（Rf0.57、Rf0.63）(0.1g/ml)had stronger antimicrobial activity on Agaricus blazei Murrill, the antimicrobial zone on Pseudomonas was 19.3mm. The bacteriostatic activity of chromatography strap of water phase was rather weaker, the water solution (0.5g/g) of amylase had better effect on pathogenic fungus of Agaricus blazei Murrill, its antimicrobial zone on Aspergillus niger, Citrinin, Trichoderma viride were 10.8 mm, 7.9 mm, 5.3 mm.

通过对日本凹顶藻以不同溶剂的粗提、乙醇提取物的极性分离、不同极性的薄层层析和多糖的提取，进行抗菌试验的研究，发现95%乙醇和V甲醇：甲苯为3∶1的提取的活性物质具有较强的抗姬松茸的病原细菌活性，乙酸乙酯相对病原细菌的抑菌强于乙醇的粗提物，而水相基本无抑菌效果，乙酸乙酯相的两个带（Rf0.57、Rf0.63）浓度为0.1g/ml具有较强抗姬松茸病原细菌的活性，对假单孢杆菌抑菌圈达到19.3mm，水相层析带的抑菌活性较弱，多糖的粗提物配成0.25g/g的水溶液对姬松茸病原真菌有较好的抑菌效果对黑曲霉、桔青霉和绿色木霉的抑菌圈分别达到10.8 mm、7.9 mm、5.3 mm。

Aqueous extract of cotton has allelopathic effect on Raphanus sativus, Brassica campestris, Lactuca indica, Brassica campestris, Echinochloa crusgall, Setaria viridis and Abutilon theophrasti and its inhibition effect gradually strengthens with increasing concentration. Aqueous extracts from stems, leaves and roots of cotton inhibit or accelerate seed germination and seedling growth, and activity of enzyme of seedling root of cotton. While aqueous extracts from stems and leaves of cotton are 0.02 gmL^(-1), root length, shoot length, lateral root number and dry weight of cotton respectively increase by 10.3%, 3.1%, 22.9% and 2.4% over the control. The above mentioned indexes are lower than the control by 34.4%, 39.2%, 40.6% and 19.0% respectively as the aqueous extracts are 0.1 gmL^(-1). At the same time, root activity and SOD activity decrease by 38.2% and 36.7%, while POD activity and MDA content increase by 52.3% and 36.5%. Growth indexes of plant receptors increase at low concentrations but decrease at high concentrations of aqueous extracts. The allelopathic effect of aqueous extract of stems and leaves is stronger than that of root.

结果表明：棉花植株水浸提液对萝卜、白菜、莴苣、油菜、稗草、狗尾草、苘麻均具有化感效应，随着作用浓度增加，抑制效应逐渐加强；棉花茎叶和根系水浸提液对棉花种子萌发、幼苗生长、幼苗根系活力、根系酶活性有抑制或促进作用，当茎叶浸提液浓度为0.02 gmL^(-1)时，棉花根长、苗高、侧根数、干重分别比对照增加10.3%、3.1%、22.9%和2.4%，当浓度为0.1 gmL^(-1)时，上述指标与对照相比分别降低34.4%、39.2%、40.6%和19.0%，而根系活力、SOD活性比对照降低38.2%、36.7%，POD活性、MDA含量较对照增加52.3%和36.5%，不同浓度棉花植株水浸提液对棉花生长呈现&低促高抑&的规律，且茎叶浸提液作用效应大于根系浸提液。

Results The peak of antibacterial activity appeared at 36-48 hafter induced by four inducements (Because many M.domestica larvae pupated,we did not observe the peak of antibacterial activity by heat-shock). The antibacterial activity of antibacterial proteins by S.aureus was best,and the antibacterial activity of antibacterial proteins by heat-shock was better than control group.

结果 各诱导组诱导家蝇幼虫产生抗菌蛋白达最大活性时间为诱导后36～48h(热诱导后家蝇幼虫很快化蛹，未观察抗菌蛋白产生高峰时间)；金黄色葡萄球菌诱导后的抗菌蛋白抗菌活性最佳，热诱导后的抗菌蛋白抗菌活性也较未诱导组好。

If I didn't care what anyone thought, I would.....

如果我不介意别人怎么看，我会。。。。

In recent decades, Chile has mandated earthquake-proofing for new structures, requiring that materials like rubber and features like counterweights be built into the architectural designs to allow buildings to bend and sway during temblors rather than break.

在最近数十年里，智利已经为兴建防震的新土建结构而对建筑材料提出要求，需要的材料类似于橡胶，而特性就象运用在建筑设计中的平衡物体，一旦应用以后，当地震发生时建筑就能弯曲摇摆。