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The PPO activity of genotype 2AL/2DL,2AL/2DH,2AH/2DL,2AH/2DH get higher one by one for one degree40-60AU/min·gand their activity level is low medium,medium,medium high,high medium,respectively;and they make activity decrease 30.1%,decrease 7.8%, increase 8.3%,increase 30.0%,respectively.8.2AL and 2DL gene make kernel PPO activity decrease 25%and 10%, respectively.2AH and 2DH gene make kernel PPO activity increase around 17%.2AL, 2DL,2AH,2DH keep PPO activity at low,medium,medium high,medium high level, respectively.

四种基因型2AL/2DL、2AL/2DH、2AH/2DL、2AH/2DH的籽粒PPO活性逐渐升高一个档次(40-60AU/min·g),且其活性分别处于低偏中、中等、中偏高、高偏中水平,可分别使活性降低30.1%、降低7.8%、升高8.3%、升高30.0%。8.2AL和2DL基因分别使籽粒PPO活性降低25%和10%。2AH和2DH基因均可使籽粒PPO活性增加17%左右。2AL、2DL、2AH、2DH基因分别使籽粒PPO活性维持在低、中等、中高、中高水平。

Therefore, systemically research on distribution and activity of fault system in the middle part of Huanghua depression is done in this paper, including the quantitative analysis of planar distribution and activity of fault system ,the anatomization of the activity and evolution of the major fault, in order to determine the relativity and cause of the fault system. Conbined with the found reservoirs and the evidences of oil and gas displaying .The relationship between fault activity time and activity characteristic, planar distribution and the displacement type of the fault system, the contact relationship of lithology with the formation of oil and gas pools is discussed and finally the faults developing model to form oil and gas pools of upper Tertiary system are found in middle part Huanghua depression.

本论文以黄骅坳陷中区为主系统的研究了断裂系统的展布和活动特征,包括断裂平面展布与活动的定量分析、主干二级断层的几何特征和活动演化历史的详细解剖,来分析断裂系统的相关性和断裂系统的成因,并与黄骅坳陷已发现的油气藏及已有的油气显示成果结合,探讨断层的活动时间和活动特点、断层的平面分布、平面和剖面组合关系、断层两盘的岩性对接关系等方面与油气成藏的关系,最终建立黄骅坳陷中区新近系油气成藏的断裂发育模式。

This study showed that the most important fitness indicator for Taiwan is the amount of physical activity, including time and intensity of participation. The first concern of government should be the increase in number of people participating in physical activity and to make exercise as part of life style. Increased amount of physical activity could reduce the risk for many chronic diseases. The yearly fitness test should focus on the amount of physical activity conducted, the socioeconomic information, and the baffler of engaging physical activity.

本研究的结果显示,当前我国最重要的课题应在於提升运动人口,培养国民运动习惯,最重要的体适能指标应为身体活动量,包括参与运动的时间和强度;增加身体活动量可降低各种慢性病罹患机率,每年的体适能检测应以调查民众身体活动量为主,并包含社会经济地位与参与身体活动的限制因素等资料,以作为制定政策的参考。

Now the research on HPL focus on dicotyledon, and the research on HPL of monocotyledon still havent been reported. In this paper, the research on cloning of rice HPL and enzyme activity assay were carried out and several result were obtained.1. Enzyme activity assay of rice HPLBased on the method of measure HPL Enzyme activity in other plants, the means on preparation of hydroperoxides and measurement rice HPL activity were set up. The 9-HPL arid 13-HPL activity of several rice varieties with different storability and different dormancy were tested.

本试验建立了水稻的HPL酶活性测定体系,测定了水稻萌发过程中的HPL酶活性,并进行了水稻HPL基因的克隆及表达研究,结果如下: 1、水稻HPL的酶活性测定在参考其它植物HPL酶活性测定方法的基础上,建立了水稻的HPL底物发生体系和酶活性测定体系,并测定了不同贮藏性水稻品种萌发过程中的种胚9-HPL、13-HPL活性和不同休眠性水稻品种种子的9-HPL、13-HPL酶活性。

Under normal temperature.the Ca2+-ATPase activity was found on the innerside of the PM.on the vacuole envelope and the chloroplast envelope,and theenzyme activity declined upon heat stress.The Ca2+-ATPase activity on the vacuoleenvelope,PM.and the chloroplast envelope in both Ca2+-and oxalate-treated peppermesophyll cells was higher than that in the control under the same condition.TheCa2+-ATPase was also found at the granum lamella,and it was activatedsignificantly by exogenous Ca2+ and oxalate treatments.Under heat stress.the Ca2+-ATPase activity declined slowly in both Ca2+- and oxalate-treated mesophyll cells.But La3+ treatment inhibited the enzyme activity under both normal condition andheat stress.

常温下生长的叶肉细胞,在质膜、液泡膜、叶绿体被膜等处有Ca2+-ATPase活性,热胁迫后酶活性下降;外源Ca2+和草酸预处理对辣椒叶肉细胞各种膜上Ca2+-ATPase活性具有促进作用,特别是定位于液泡膜、质膜和叶绿体被膜上的酶活性明显比对照提高;在叶绿体基粒和基质片层上也有酶活性,并且Ca2+和草酸预处理对该部位上的酶活性激活作用更明显;La3+处理的作用与Ca2+和草酸处理的效果相反。

For a peptide to act as part of the host defense system or to be of pharmaceutical interest, high antimicrobial activity must be combined by low hemolytic activity. Our results indicated that the antimicrobial activity increased with increasing charge and polar angle, with charge of +4 and polar angle of 140° exhibiting the highest antibacterial and membrane permeabilizing activity. Higher hydrophobicity and hydrophobic moment correlated with increased hemolytic activity, thus decreasing selectivity dramatically.

胜肽要扮演宿主免疫机制的一部分或要成为新型态的抗生素不仅要有强大的抗菌活性还需要有高度的选择性,由本实验的结果发现,当抗菌胜肽的正电荷及极性角度增加时,胜肽之抗菌活性会随之增加,当抗菌胜肽的电荷为+4而极性角度为 140°时,胜肽有最强之抗菌能力及膜渗透活性;而随著胜肽疏水性以及疏水性矩的增加,会增加溶血活性,结果反而降低了抗菌胜肽的选择性。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明:成功构建了pET22b-lysB表达载体,并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白;His-LysB具有分解脂肪的能力,属于脂肪酶;生物化学特性分析表明:丁酸对硝基苯为水解底物,His-Lys热稳定性不佳,30℃以下比较稳定,随着温度的升高,稳定性逐渐降低;该蛋白具有较高的pH值适应性,pH 5.0~9.5范围内稳定性较高;在23℃和pH 7.5时酶活力最高,其比酶活为1.3 U/mg;金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

Therewas a significant characteristic of the vertical distribution of soil enzyme activity,the relationship analysis indicated that there was a significant positive relationshipbetween the catalase activity and OM,and total N,and effective P、K,and ex-changible Mg,there was a negative relationship between the catalase activity andBase Situatiou percentage,and Total Contents of Base;the activity of the polyphe-nol oxidase was positively related with OM,and negatively related with total N andexchangible Ca clearly;The urease activity decreased with CPR increasing,and thechange of the former two kinds of soil enzyme actirity was not significantly withCPR increasing,It is possible to be related with maned disturbance frequently.

随着代次增加,有机质和交换性Ca下降明显;土壤的CEC和交换性酸分别下降了8.8—16.1%和6.8—18.5%,因此,其养分总量亦逐代降低,其中有机质下降幅度最大;土壤酶的活性具有明显的垂直分布特征,相关分析表明,过氧化氢酶活性与土壤OM、全N、有效P、K和交换性Mg有极显著的正相关,而与盐基总量、盐基饱和度呈明显的负相关。多酚氧化酶活性与OM呈显著负相关,与全N、交换性Ca有显著相关。脲酶活性则有随连栽代次增加而有下降趋势,前两种酶在代次之间变化不明显,可能与频繁的土壤干扰有关。

The theoretical analysis shows that the wave-activity relation presented a nonconservative form in which the pseudoenergy wave-activity density is composed of disturbance kinetic energy, available potential energy and buoyant energy. The local change of pseudoenergy wave-activity density depends on the combination of shear of zonal basic flow, Coriolis force work and wave-activity source or sink as well as wave-activity flux divergence.

理论分析表明,拟能量波作用方程具有非守恒形式,其中的拟能量波作用密度主要由扰动动能、有效位能和浮力能三部分组成;拟能量波作用密度局地变化除了受拟能量波作用通量散度影响之外,纬向基本气流切变、科氏力作功以及由非绝热加热和水汽相变所构成的波作用源汇项对其也都有贡献。

Activity of s AChE and GSTs was elevated in 4 strains,of which the activity of AChE and GSTs in KFQ strain and BYS strain was 1.57 fold and 2.33 fold respectively compared with susceptible strain.Activity of AChE and GSTs was decreased in one strain,activity of AChE was elevated but activity of GSTs was decreased in other 3 strains.

有4种品系可使AChE和GSTs活性提高,其中KFQ品系、LD品系与敏感品系相比,AChE的活性分别提高了1.57倍和1.44倍;BYS品系使GSTs活性提高了2.33倍;有3种品系可使AChE活性提高,使GSTs活性下降;另有一种品系使AChE和GSTs活性均下降。

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