查询词典 current amplification

The influence of various factors on amplification outcome was observed by gelose gel electrophoresis.

目的探索多重PCR体系中各成分的最适组合，以便在真菌性角膜炎的诊断中获得准确、可靠的结果。

The data of hui ethnic group were generated through co amplification, genescan, genotyping, and genetic distribution analysis.

每个基因座的引物分别用fam，hex或tamra荧光标记，通过复合扩增、基因扫描、基因分型和遗传多态性分析获得数据。

Gene frequency/genet ; gene amplification/genet ; polymerase chain reaction/methods

基因频率／遗传学；基因扩增／遗传学；聚合酶链反应／方法学

The RAPD system of Gladiolus was optimized. Of the 12 random primers analyzed in this research, 9 primers can show steady and polymorphic amplification profiles.

建立了适用于唐菖蒲RAPD分子标记的反应体系，从12条随机引物中筛选出9条可扩增出多态性条带的引物。

PCR Kit. Primers and probes (Shanghai Public Health Synthesis). PCR Amplification (Biometra Personal PCR system, Germany), biochips plotter (Micro Grind II 600, Biorobtics Ltd, England), confocal laser scanner Gene TACTMLS IV, Gnomic Solutions Inc.

PCR试剂盒引物与探针 PCR扩增仪(Biometra Personal PCR system，Germany)，生物芯片点样仪(MicroGrindⅡ600，Biorobtics Ltd，England)，激光共聚焦扫描仪Gene TAC~(TMLSⅣ，Gnomic Solutions Inc。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

The Hall probe measurement circuit is mainly composed by the Hall probe, the non-idiostatic offset circuit, the signal amplification circuit, the automatic range conversion circuit and the A/D transducer circuit.

磁场测量装置主要由霍尔探头、不等位电势补偿电路、信号放大电路、量程自动切换电路、A/D转换器组成。

The system consists of signal acquisition systems, signal processing, show circuit and control circuits, and other components, through the acquisition by Hall sensor voltage signal to the amplifier will signal amplification, and then in the A / D converter after the digital signal into the microcontroller , Through the SCM software programming by the magnetic field signal, the final adoption of LED display to the magnetic field strength.

该系统由信号采集系统、信号处理、显示电路和控制电路等组成，通过霍尔传感器采集得到电压信号，再经放大器将信号放大，然后在A/D转换后将数字信号送入单片机，通过单片机软件编程得到磁场信号，最后通过LED显示出磁场强度。

The non-uniform slow wave structures of the broad-band high power TWTs are studed for the suppression of the backward-wave oscillation and second harmonic amplification. and for the interaction efficiency improving.

本文主要利用非均匀输出线路来研究宽带高功率行波管的返波抑制、二次谐波抑制和提高电子互作用效率的问题，旨在通过本论文的研究，为宽带高功率行波管的研制提供可靠的理论依据和设计指导。

Its blend of blues and rock influences with distorted amplification made it one of the pivotal records in the evolution of heavy metal music.

这张专辑混合了布鲁斯音乐跟摇滚乐又受到被曲解的扩大，使它成为影响重金属摇滚发展的关键唱片之一。

There are different types of personal loans available.

有许多不同类型的个人贷款优惠。

The above mentioned teaching model of spiral rising can be used to avoid rigidness in teaching.

上述螺旋式上升的教学模式，可以避免教学形成模式後的僵化现象。