查询词典 current amplification

Secondly, there is a phenomenon called amplification that enables a very tiny field to produce a large effect.

其次，有一种现象被称为放大，使一个很小的领域产生很大影响。

The transformants harboring different copies of amyL were developed on kanamycin by using homolog-mediated chromosomal amplification of a-amylase gene.

licheniformis B0204染色体中的同源整合与高温a-淀粉酶编码基因amyL的扩增，由此获得了携带多个amyL拷贝的转化子。

The total genomic DNA of diseased apple leaves was rapidly obtained by modified CTAB method. Through PCR amplification assay, the pathogen of apple scab can be specifically detected from diseased apple leaf tissues and no symptom appearance of apple leaf tissues with pathogen incubation.

采用改进的CTAB法，快速提取发病叶片组织的DNA，并结合PCR检测技术，可从苹果黑星病显症叶片以及未显症而处于潜育期的叶片组织中特异性地检测到苹果黑星病菌。

The constructed pcDNA3-IL-15 plasmid was identified by Bgl Ⅱ digestion,PCR amplification and sequenceanalysis respectively,to ensure that the hIL-15 cDNA was properly inserted into thevector and the sequence was correct.Then the pcDNA3-IL-15 plasmid was instantlyexpressed in COS-7 cells by means of lipofectamine transfection,and followed by astable expression in Chinese hamster oval cells.8 positive cell colonies wereobtained after the G418 selection,designated CHOA~H cells.

经过BglⅡ酶解分析，PCR扩增鉴定和DNA测序分析，证明IL-15 cDNA已正确插入克隆载体且序列正确后，采用脂质体法，首先将pcDNA3-IL-15重组质粒转染至非洲绿猴肾细胞（COS-7）做瞬时表达，再转染至中国仓鼠卵巢细胞进行稳定表达。

Wave Settings allow channel selection, amplification and more than 30 output audio formats.

波设置允许通道选择，扩增和30多个输出音频格式。

Asymmetric autocatalysis combined with chirality amplification has been made the new concept in chirality origin .

不对称自催化反应结合手性放大作用，使人们对手性起源有了新的认识。

Asymmetric autocatalysis intrinsically has the following advantages: chiral amplification of production, high reaction efficiency, easy purification of the product, consecutive reaction, and is a new aspect of chiral synthesis.

不对称自催化具有手性自动放大、反应活性较高、产物处理较易、反应体系连续等特点，是不对称化学的一个新的领域。

in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要：为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法，根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物，进行多重pcr扩增，并对多重pcr反应体系进行优化。

In the present study,using PCR amplification and automatic sequencing of EDA gene coding region,we report five mutations identified in 6 Chinese families with XLHED, including three novel ones:p.M1T,p.L62P and

其中3个点突变(p.M1T、p.L62P和p.G195E)为国际首次报道的新突变。

The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

There are different types of personal loans available.

有许多不同类型的个人贷款优惠。

The above mentioned teaching model of spiral rising can be used to avoid rigidness in teaching.

上述螺旋式上升的教学模式，可以避免教学形成模式後的僵化现象。