查询词典 current amplification

22 Of 22 pairs EST-SSR primers from Chinese cabbage/rape respectively had amplification products in soybean,wheat and maize,the rate of amplification was 81.8%,95.4%,100%,respectively;10 of 22 pairs EST-SSR primers showed polymorphism in monocotyledons wheat and maize,13 in dicotyledons oilseed rape and soybean,7 in both monocotyledonous and dicotyledonous crops.

（1）30对小麦EST-SSR引物中，有29，28和26对引物分别在玉米、油菜和大豆中有扩增产物，可扩增率分别为96.7%，93.3%和86.7%；其中分别有12对和9对引物在单子叶作物小麦和玉米及双子叶作物大豆和油菜中的扩增产物显示多态性，有4对引物在4种作物中的扩增产物均显示多态性。

(1)29,28,26 of 30 pairs of EST-SSR primers from wheat respectively had amplification products in maize,oilseed rape and soybean,the rate of amplification was 96.7%,93.3%,86.7%,respectively;12 of 30 pairs EST-SSR primers from wheat showed polymorphism in monocotyledons wheat and maize,9 in dicotyledons oilseed rape and soybean,and 4 in both monocotyledonous and dicotyledonous crops.

基金项目：国家生命科学与技术人才培养基地科技创新基金项目(2007088)；国家油菜产业化项目科学家岗位"化杀岗位"（nycytx-00505）；国家自然科学基金项目(30871578)中文摘要：探讨单、双子叶作物间EST-SSR引物和标记的通用性。选取30对小麦、8对油菜和14对白菜的EST-SSR引物，分别以10个小麦、10个油菜、5个大豆品种和5个玉米自交系的基因组DNA为模板，进行PCR扩增及扩增产物的多态性分析。

The method is based on real time reverse-transcription-PCR amplification of mRNA to detect viable Listeria monocytogenes by specific hlyA gene. This method can overcome the false-positive caused by amplification of nonviable Listeria monocytogenes in the detection process.

为克服传统PCR检测的假阳性问题，有效的检测单增李斯特活菌，本研究提取单增李斯特菌的总RNA并进行反转录，以单增李斯特菌的必要毒力基因hlyA为靶基因，设计特异性引物和TaqMan探针进行实时PCR检测，研究了检测的特异性和灵敏度，考查了对人工污染牛乳进行检测的应用性。

By analyzing the optical characteristic of the off-axis and multi-pass amplified high power solid-state laser facility,using the theory of binary tree tracing and matrix optics,and dividing the system into four parts that include cavity amplification part,boost amplification part,beam reverser and beam output part,the ghost image are analyzed and traced.Main of ghost image that are devastating are given in all parts.

为了有效地避免鬼像破坏，针对采用离轴多程放大的高功率固体激光实验装置的光路特点，利用"光路二叉树"原理和"矩阵光学"方法，将全光路"单元化"为主放大、助推放大、反转器和输出4个部分进行光线追迹和鬼像分析，给出了各个部分的主要鬼像分布。

Seeded amplification of colored conical emission and two-dimensional multicolored transverse arrays as well as simultaneously up-converted parametric amplification have been demonstrated using spectral synthesis to obtain sub-fs pulses.

基于分子调制过程，文章作者提出并发展了一系列亚飞秒脉冲产生和操控的技术；围绕频谱合成方案，利用级联二阶非线性效应，实现了多色圆锥辐射注入放大和二维多色阵列及其参量上转换放大；同时，还开展了基于飞秒振荡器及外腔增强技术的高重复频率高次谐波产生与输出耦合的相关研究。

The reaction system of AFLP (Amplified Fragment Length Polymorphism) in genus Eriobotrya was studied and the AFLP analysis system was established by detected the results of several reactions such as DNA extraction, enzymes restriction, adapter-ligation, pre-amplification and selective amplification; the main factors which affected AFLP analysis were analyzed.

通过检测AFLP分析中DNA提取、酶切及连接、预扩增和选择性扩增的结果，对影响AFLP分析的主要因子进行分析，建立了AFLP分析体系。

Using the amplification transfer function of type Ⅰ BBO crystal, the theoretical study of spatial properties of the optical parametric fluorescence generated by homochromatic signal and polychromatic signal in optical parametric amplification is investigated, The numerical simulation results demonstrate that when the homochromatic signal is injected into optical parametric amplifier, the fluorescence signal is first emitted in a cone and then in a ring while centering on the pump wave direction.

采用第Ⅰ类相位匹配BBO晶体的放大传递函数方法，分别对光参量放大过程中单波长信号光注入和多波长信号光注入出现的参量荧光的空间特性进行了详细的理论研究。

GRP can also make mice's MNC and hematopoietic stem cells from human's umbilical cord blood amplification. We use colony formation to analysis the linear relation during the cells'growth and the medicine's dosage, examine the amplification of CFU-GM induced by hypothallus and its supernatant, RT-PCR is used again to check three cytokines'genes.

发现GRP对小鼠MNC扩增有作用，从而将GRP运用到人脐带血MNC的扩增分化上，用集落形成法分析药物与扩增的线性关系，基质层、基质上清对CFU-GM的影响，用RT-PCR技术检测基质层细胞生长因子基因的扩增。

During our strategy, two linear amplification approaches are employed, ligase detection reaction and an isothermal amplification (cooperation by DNA polymerase and nicking enzyme).

连接产物由于在DNA聚合酶和限制性内切酶的共同作用下，发生等温扩增反应，扩增出大量的血红素的适配子，适配子与血红素结合，形成的复合物催化鲁米诺和双氧水的化学反应。

Result In the Identification of ISSR molecular markers, 3 primers in 20 of being chose had better amplification effect. Optimization results representative of C. grandis Osbeck showed that primers 826 for the optimum temperature of Rutaceae plants amplification was 52.0℃, and the best cycle time was 35 cycles.

结果］选用的20个引物中有3种引物扩增效果较好，以柚为代表的优化结果表明，引物826对芸香科植物扩增的最适退火温度为52.0℃，最佳循环次数为35个循环。

Now he worked in ajoint venture.

现在他在一家合资企业工作。

More troops in Afghanistan won't change any of the foregoing.

更多的在阿富汗的军队也不会改变之前的状况。