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Results After culturing with As 2O 3,PIEC present paste wall cells decrease,float,vacuolation,fat granule increase,and apoptotic features:i.e.cell membrane integrity,chromatin condensation,apoptotic body formation,etc.

为此我们采用猪髂动脉内皮细胞为对象,研究不同浓度As2 O3 对PIEC生长的影响,探讨介入放射学方法导入As2 O3 治疗实体瘤的作用机制。

This expression distribution suggests that COCH gene may contribute to maintain inner ear connective tissue extracellular matrix architecture, and do not support the postulation that gene might play an inner ear immune defensive function. 2. In vitro, the culturing spiral ligament type Ⅰ fibrocytes expressed COCH gene and POU3F4/brn4 gene, which were checked by RT-PCR and Western blot, and expression level did not chang with in 15 generations.

小鼠内耳COCH基因原位表达分布的观察结果提示,基因的功能与维持内耳间质组织细胞外基质的结构稳定有关,不支持COCH基因的功能与内耳免疫应答反应有关的推论。

In paper, it expatiates the connotation , class and function of the legal consciousness of the people in the content, historic reviewing and important meaning of managing state affair according to the laws. From the different angel , it do some thorough fondues analysis to the current situation of the legal consciousness of our country抯 people. Then it bring forward some measure of pertinence in the culturing and boosting the legal consciousness of the people.

本文从依法治国方略的涵义、历史回顾及重要意义的论述入手,又对公民法律意识的内涵、分类及功能作以阐述,在从不同角度对我国当前的公民法律意识现状进行描述的基础上,做出了深入具体的成因分析,并进而有针对性地提出了培养与提高公民法律意识的若干措施。

Eichhornia crassipes was water cultured in 0, 0.01, 0.t and 0.3mgCd/L. Throughout the experiment, plants in control group grew ordinarily. Root-shaped stalk is short and strong with slender majority of fibrous roots black, Leaves were dark green and smooth. Gourd-shaped balloon is inflated and round. 80% of the plants opened bright purple flowers for 3-5 days and yielded egg-shaped fruit after 15 culturing days.

在镉浓度分别为0,0.01,0.1和0.3mg/L水培溶液中,对照组中凤眼莲在整个实验期内生长正常,根状茎粗短,密生多数细长须根,呈黑色;叶片深绿色,光滑,葫芦状的气囊膨大且圆;在培养15天时80%的植物开紫色亮丽的花朵,花期3—5天后结卵圆形果。

In this essay ,by mean of culturing with nutritional solution of different salt concentration (NaCl, mmol/L), and investigated the salt tolerance of four halophytes in two aspects of microstructure and physiological indexes.

本文采用不同盐浓度(NaCl, mmol/L)营养液培养的方法,从形态显微结构和生理生化指标两方面对四种植物的抗盐性进行了研究。

Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.

并以纯化的M蛋白为抗原,免疫BALB/c小鼠,按常规方法进行融合,间接ELISA方法进行筛选,采用有限稀释法,经过三次克隆,最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5),分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类,三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。

Results:① Wnt7a was expressed in hEFCs and ICR mEFCs after culture normal karyotype hESCs, but not expressed in control and in hEFCs and ICR mEFCs after culturing abnormal karyotype hESCs with karyotypic change in chromosome NO.

Wnt9a的表达量在未培养正常hESCs的hEFs中表达量高,而在养hESCs后的hEFs中表达量低。

The cell biological features were observed by inverted phase contrast microscope, l ight microscope, electron microscope, cell vital ity assay, cell growth curve and cells staining after harvest and during the periods of culturing the primary, the 1st passage and 2nd passage.

分别在取材后、原代、第1 代、第2 代细胞培养期间,进行髓核细胞活力测定;爬片培养后进行甲苯胺蓝、HE、聚集蛋白聚糖番红O、Ⅰ型及Ⅱ型胶原免疫组织化学染色观察;MTT 法绘制髓核细胞生长曲线,并行原代及第2 代细胞透射电镜观察,对体外细胞的生物学特性进行研究。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用,按美国ASTM标准制备NiTi、317L合金的金属浸提液;&组织块培养法&原代培养食管壁疤痕的成纤维细胞,传代后以金属浸提液进行培养,分组后分别培养4、24、48、72 h,MTT法检测不同培养时间后Fb增殖功能的变化;按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验,即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间,术后2、12周取出包埋组织,分析试件周围组织的病理变化,并进行胶原纤维染色,观察纤维形成状况。

By culturing on malt extract agar medium with temper

结论]红腐乳中分离的纯培养为红曲属真菌。

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We will see more and more activist government policies that distinguish economic activities according to the nationality of the actors.

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