查询词典 culturing

We have previously established The high frequency regeneration system by using the young leaves of Platanus acenfoliaWill as the culturing materia.

我们已经建立了利用悬铃木叶片培养诱导植株的高频再生体系。

The foundation for the further development of new type of material for Pleurotus geesteranus culturing was laid.

结论］为进一步发展秀珍菇栽培新代料打下了基础。

The technique of hanging culture Portunus trituberculatus in ponds of culturing juvenile is advanced in China because of its new pattern and remarkable result.

该项技术利用空闲的河蟹幼蟹培育池，采用笼式吊养全雌梭子蟹，方式新颖，效果明显，技术水平国内先进

MethodsTo pre-treat buds at the temperature of 4℃ for different hours,place them on the MS mediums containing different hormones and different concentration of sucrose ,and before culturing the buds at the temperature of 25℃ in the darkness,preculture them at 33℃ for different hours. Record the rate of induction two weeks later.

方法青蒿花药在进行不同时间的低温前处理后，置于含不同激素和蔗糖浓度的MS培养基上，再进行不同时间的高温后处理，然后转入25℃黑暗培养，统计愈伤诱导率。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Results The purity and following treatment of inclusion bodies of CPB were influenced by the culturing temperature after the inducement with IPTG. The solubility of inclusion bodies of rCPB in 10mol/L urea was 2~3 times higher than in 8 mol/L urea, and the renature solution with 0.75%β-ME in 10mol/L urea could increase the solubility of inclusion bodies of rCPB, comparing with that in 10mol/L urea simply.

结果诱导后生长温度不同对rCPB包涵体的纯度及后期处理均有影响；rCPB包涵体在10 mol/L尿素溶液中的溶解度比在8 mol/L 尿素溶液中提高2~3倍；添加0.75%β-巯基乙醇能显著改善rCPB包涵体的溶解效果。

Methods〕E coli and salmonellae were cultured together with different culturing media.Then the enriching effect of each enrichment medium was determined.

方法〕用一定数量的大肠埃希菌与不同数量种类的沙门菌混合培养，观察不同增菌液的增菌效果。

By culturing human brain microvascular endothelial cells and glioma cells separatedly and simutaneously ,and then analyzing their pre- and post-cultune FasL levels, we study the effect of the grouth of glioma cells upon FasL level of human brain microvascular endothelial cells.

我们通过将人脑毛细血管内皮细胞与胶质瘤细胞分别培养及共同培养，对培养前后人脑毛细血管内皮细胞与胶质瘤细胞FasL表达水平的分析，来探讨胶质瘤细胞的生长对人脑毛细血管内皮细胞FasL表达的影响。

Fig. 5 Allogenic T cell stimulatory function of HDC and BM-DC after different culturing time.

图5 不同培养时间的 HDC 和 BM-DC刺激同种异体小鼠 T 细胞增生。

The big eared hedgehog, the little owl, the sand gecko, the three-toed jerboa, and every show needs a villain - saw-scale - the viper!

大耳刺猬，小猫头鹰，沙壁虎，三趾跳鼠，每场戏都需要反派角色：蝰蛇。

Plasmodiophora brassicae ; resting spores ; density gradient centrifugation of sucrose ; ultrasonic disruption

根肿；休眠孢子；蔗糖密度梯度离心；超声波破碎