查询词典 culturing

The retinal explants could maintain their structure in short term of culturing. This made the transplantation into subretinal space in vitro possible.

视网膜片在体外短期培养，不影响其组织结构和细胞特性，短期的体外模拟视网膜下腔移植具有可行性。

The EC_(50) of QKL against the formation of syncytia of MT-2 induced by HIV-1_ was 1/198.02 with a SI of 5.36. By detecting the survival rate of co-culturing H9/HIV-1_ cells and MT-2 cells or MT-2 cell direct infected by HIV-1_ QKL was found to be protective to cells. The EC_(50) was 1/166.67 and 1/144.93 with SI of 4.51 and 3.92 respectively. The EC_(50) of QKL against P24 antigen production was 1/175.44 with SI of 4.75. The drug serum of QKL was also found to be effective to inhibit the cell fusion and protect cells infected by HIV-1_.

结果：QKL对H9／HIV-1_细胞和MT-2细胞的CC_(50)分别为1／50.76和1／36.97；抑制H9／HIV-1_细胞和MT-2细胞早期融合的EC_(50)=1／235.29，SI=6.36；抑制HIV-1_细胞诱导MT-2细胞形成合胞体的EC_(50)=1／198.02，SI=5.36；H9／HIV-1_细胞和MT-2细胞混合培养和HIV-1_感染MT-2细胞时，QKL保护病毒感染细胞免于死亡的EC_(50)分别为1／166.67和1／144.93，SI分别为4.51和3.92；抑制p24抗原产生的EC_(50)=1／175.44，SI=4.75；QKL药物血清也可抑制H9／HIV-1_细胞和MT-2细胞的早期融合，保护细胞免于死亡。

Objective To establish a method for isolating and culturing the tonsillar lymphocytes,analyze the distribution of lymphocyte phenotypes so as to get some experimental data for peripheral lymphocyte research.

目的 建立人扁桃体淋巴细胞的分离培养方法，研究淋巴细胞的表型分布，为外周淋巴细胞的研究提供实验数据。

Experiment of culturing Tricholoma giganteum by using rapeseed coat as culture material was carried out.

用菜籽皮作培养料进行了巨大口蘑的栽培对比试验。

And probable mechanism for the solubility differences was illuminated by the analysis of unreduced SDS-PAGE. Results The purity and following treatment of inclusion bodies of CPB were influenced by the culturing temperature after the inducement with IPTG.

结果诱导后生长温度不同对rCPB包涵体的纯度及后期处理均有影响；rCPB包涵体在10 mol/L尿素溶液中的溶解度比在8 mol/L 尿素溶液中提高2~3倍；添加0.75%β-巯基乙醇能显著改善rCPB包涵体的溶解效果。

Of CBMC can be purified by Mini-MACS as CD34〓 stem cells. B. The number of CD34〓 stem cells can expand to 40.24±9. 86 fold after 14 days. C. No matter in the expression of CD1a, CD80, CD86, and HLA-DR, or in the function of stimulating xenogenous lymphocyte proliferation, there was no difference between CD34+ stem cell DCs or monocyte DCs. D. The percentage of CD3〓CD56〓 cells is the same in CIK cells co-culture with DCs transfected with SKOV3 RNA, CIK cells co-culture with DCs, and CIK cells. E. The expansion rate of CIK cells can be accelerated by co-culturing with loaded or unloaded DCs. However, the expansion rate between loaded or unloaded group is the same. F. The strongest cytotoxicity against SKOV3 cell line was achieved by CIK cells co-cultured with DCs loaded with SKOV3 RNA.

结果：1、Mini-MACS分选系统可自CBMC中提取0.78±0.31%的CD34〓细胞；2、体外培养14天后可获得原始CD34〓细胞量40.24±9.86倍的细胞；3、不论在CD1a、CD80、CD86及HLA-DR的表达上，或是刺激异体淋巴细胞增生的功能上，脐带血CD34〓细胞与单核细胞来源的DC都没有差别；4、CIK细胞中CD3〓CD56〓双阳性的表达率在与RNA转染DC共培养的CIK细胞组、与DC共培养的CIK细胞组及单纯CIK细胞组3组间比较无差异；5、脐带血CIK细胞增殖显著，培养14天时可扩增18.18±5.59倍，培养21天时可扩增35.02±6.30倍；5、与未转染或转染DC共培养的CIK细胞在培养第14天后增殖速率大于单纯CIK细胞。

D. The function of stimulating xenogenous lymphocyte proliferation was the same between peripheral DCs and ascites PCs. E. The percentage of CD3〓CD56〓 cells was the same in CIK cells co-culture with DCs transfected with SKOV3 RNA, CIK cells co-culture with DCs, and CIK cells. F. The expansion rate of CIK cells can be accelerated by co-culturing with loaded or unloaded DCs. However, the expansion rate between loaded or unloaded group is the same. F. The strongest cytotoxicity against SKOV3 cell line was achieved by CIK cells co-cultured with DCs loaded with SKOV3 lysate.

结果：1、腹水可获得0.83±0.24×10〓个AMC/ml，单核细胞有0.74±0.25×10〓个/ml；2、卵巢癌患者外周血可获得0.87±0.20×10〓个AMC/ml，单核细胞有0.92±0.17×10〓个/ml；3、除CD86外周血单核细胞来源DC表达较高以外，其他表面分子在不同来源DC间没有统计学差异；4、不同来源DC的异基因刺激能力没有差异；5、与负载或未负载卵巢癌抗原的DC共培养并不能提高CIK细胞群中CD3〓CD56〓细胞的数量；6、CIK细胞增殖显著，培养14天时可扩增19.18±4.70倍，培养21天时可扩增35.82±4.36倍；7、与未负载或负载DC共培养的CIK细胞在培养第14天后增殖速率大于单纯CIK细胞。

The experimental result shows that the main determinative factor is the temperature of the sea water for the growth after the transplantation; the optimal temperature for the growth of Zostera marina L. is 10~20℃; it is strongly salt-tolerant and it can grow at the salinity range of 20‰~48‰ normally; its rhizome is the optimal organ for culturing and the optimal hormone concentration for the calluses induction is 2mg/L or so.

实验结果表明，大叶藻移植后生长的主要制约因素是海水温度；大叶藻生长的最适温度是10～20℃；大叶藻对盐度的耐受性很强，它可以在盐度为20‰～48‰的范围内正常生长；地下茎是组织培养的最适材料；诱导愈伤组织的最适激素(2，4-D)浓度为2mg/L。

From the study above, the biodegrading ability and effects of EM on domestic sewage organics, the resistance of EM on some regular heavy metal ions in wastewater, the culturing and domestication of EM active sludge under the condition of both self inoculation and...

试验结果表明：(1)当水温为25℃、HRT为48hr，菌液投加量为1／10000曝气时间为HRT／3，进水pH为8.0时，EM可表现出良好降解生活污水有机质的能力，CODcr、BOD5去除率分别达到75.92％和89.57％。

From the study above, the biodegrading ability and effects of EM on domestic sewage organics, the resistance of EM on some regular heavy metal ions in wastewater, the culturing and domestication of EM active sludge under the condition of both self inoculation and digest...

试验结果表明：(1)当水温为25℃、HRT为48hr，菌液投加量为1／10000曝气时间为HRT／3，进水pH为8.0时，EM可表现出良好降解生活污水有机质的能力，CODcr、BOD5去除率分别达到75.92％和89.57％。

We will see more and more activist government policies that distinguish economic activities according to the nationality of the actors.

我们将看到越来越多激进的政府政策，这些政策根据企业的国籍来区分经济活动。

If we can prove the independence of an axiomatic system, then we can guarantee the conciseness of this system, ie.

如果能够证明某个一阶谓词系统的独立性，那么就能够保证该系统的建立是精炼的，即没有任何一条公理或推理规则是多余的。