查询词典 culturing

Different types of sample need different culturing and one eyen needs two or more than two methods when necessary to increase the detecting rate of Lm in the PCR rapid detection.

不同样品类型要用不同的增菌法，必要时用2种以上力法相结合，提高食品中Lm的检出率。

After that, the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article, in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos.1 Effects of the reducing injury methods on IVF of cryopreservated oocytesThe result of the three methods, choosing the right time oocytes, using minimum size sample vitrification and culturing after thawing, showed that these ways could partly maintained the fertilizable competences of cryopreservated bovine oocytes.

减轻损伤方案对冷冻卵母细胞IVF的影响使用选择合适时期的卵母细胞进行冷冻；采用最小样本量玻璃化冷冻方法进行冷冻；在解冻后先孵育再进行体外受精等三种方案能够部分维持或恢复冷冻牛卵母细胞IVF受精能力。

The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally.

向血管平滑肌组织块中，加入用DMEM配制的0.15％胶原酶II溶液，水浴箱中作用1.5h，至组织呈絮状，用0.02％的EDTA清洗，然后，加入0.125％胰蛋白酶溶液，于37℃水浴振荡消化，即可得到分散的平滑肌细胞，用10％新生牛血清的培养液终止酶的作用。加入含20％新生牛血清的培养液，调节细胞密度为5×104～5×105个/L，接种，放入5％CO2、37℃培养箱中培养。当细胞达到70％～80％融合时即可按常规方法传代。

The best explant in the culture was brown leaf,and the explant sterilized in 0.1% aqueous mercuric chloride for 5min has the best effect;At the initial stage in induction,3% glucosan have the best effect following with 1% glucosan+2%sucrose are optimum in the process of culturing;the optimum culture medium is 1/2MS; the preferable medium of callus is 1/2MS+ KT(0.1mg/L)+6-BA(0.05mg/L)+2.4-D(0.4mg/l);differentiation1/2MS+6-BA(0.3mg/l)+NAA0.2+KT(0.05mg/l);and the preferable medium of rooting is :1/2MS+NAA(0.05mg/L).

安祖花组织培养的最佳外植体为棕色叶片；最佳消毒时间为0.1%的升汞消毒5min；最佳碳源：诱导初期3%的葡萄糖的诱导效果最佳，后期培养以2%蔗糖+1%葡萄糖的培养效果较好；最佳基本培养基为1/2MS。

Results The results indicated that the cultured human alveolar bone origin cells from 3 patients could successfully express the osteoblasts phenotype in single layered culturing after stimulated by β-glycerophosphate and Dexamethasone.

结果自3人下颌齿槽骨分离培养的齿槽骨细胞在加入β-磷酸甘油和地塞米松刺激的体外培养条件下，均可成功表达成骨细胞的表现型，与同源成纤维细胞相比，差异有显著性(P.05)。

Methods In vitro CFU-GM culturing was used to study the effect of adult serum and CB serum on hemapoietic progenitor cells of normal bone mar row and children with AL in period of PCBS.

采用体外粒单细胞集落形成单位培养法，观察正常成人血清和脐血清，对正常造血细胞和急性白血病化疗后抑制期骨髓造血细胞增殖的影响。

Studied fungi diversity in the guts of larval Hepialus gonggaensis using culture-independent and traditional culturing methods.

分析贡嘎蝠蛾肠道幼虫肠道真菌多样性。

Heterogenic DM/posterior stroma layer is a good carrier for culturing corneal endothelium, which provides a donor material for endothelium cells transplantation.

角膜内皮细胞能够在异种DM／基质载体上良好生长，该载体有可能成为培养内皮细胞应用於临床移植的供体材料。

These are main problems of eutrophication.In this paper we selected alga that suits to grow under different concentration of nutrition, after selecting seed, domesticating, culturing alga purely in the lab, we gained Hydrodictyon reticulatum and microalga, most of which are Miorocystis and chlorella.

本文选取在不同营养水平条件下适合生长的藻类物种，在实验室进行选种、驯化和纯化培养，得到水网藻和微藻(主要为微囊藻Miorocystis、小球藻chlorella)；以实验室培养的生长速度快、生产能力高的水网藻为原料，制备一系列环境友好性光敏物质——叶绿素及其衍生物，运用光敏氧化分光光度法测定活性氧(O_2~-，H_2O_2，·OH，和~1O_2)，根据其光敏产生活性氧效率，进行光敏物质的筛选；选用叶绿素铜及其钠盐，以微藻为实验控制对象，以Cu~(2+)作培养对照，在相同光照、营养条件下进行控藻实验。

Using synchro drosophila as material, pouring purslane liquid or hydroxyketone of different density into substrate,after culturing,anti-decrepit effect can be observed.

用同步果蝇为材料，在培养基中添加不同浓度的马齿苋水提液或醇水提液，观察到了马齿苋的抗衰老效应，并且可以影响果蝇幼虫期的长短及果蝇的繁殖力。

We will see more and more activist government policies that distinguish economic activities according to the nationality of the actors.

我们将看到越来越多激进的政府政策，这些政策根据企业的国籍来区分经济活动。

If we can prove the independence of an axiomatic system, then we can guarantee the conciseness of this system, ie.

如果能够证明某个一阶谓词系统的独立性，那么就能够保证该系统的建立是精炼的，即没有任何一条公理或推理规则是多余的。