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In this study, a recombinant plasmid generating short hairpin RNA was constructed — To construct the recombinant plasmid carrying shRNA to HPV6bL1 and analyze the nucleicacid sequence for further searching new gene therapy method of cauliflower excrescence.

目前对于尖锐湿疣仍无有效的病毒特异性防治措施来保护人类免受其危害，而基因疫苗则为预防HPV感染提供了可能性；L1是HPV6型主要的衣壳蛋白，具有较强的免疫原性，可刺激机体产生保护性的中和抗体。

The aim is to construct an exemplary building providing the highest-possible level of environmental technology.

这样做的目的是建立一个模范的建设提供环保技术的最高可能的水平。

In the studying method, this text braid with the viewpoint of cognitive linguistics and culture linguistics (Two all involve the thought method), and follow two clues that were mutually connections, the one is how to construct the basic colorific phrase, the synthesize colorific phrase and the phrase contained colorific word by the colorific etyma, the two is the thought method -borrowing the thing assumes the color, imagism, and take shape to analogy, how to engender function in the process for built phrase, and select correlated exemplum to evolve to analyze.

方法上结合了认知语言学和文化语言学的观点，追循两条相关联的线索：一是色彩语素如何构造基本色彩词、合成色彩词，二是汉民族文化中特有的"借物呈色"、"意向性"、"取象类比"的思维方式在造词过程中如何起作用，选取相关例证展开分析。

In view of describing latent variables influence which are faced to the model of relationship between customer satisfaction degree and its influence factors, integrating the respective characteristic of partial least squares method and the structure equation model, this paper proposed the question that the structure equation model application in service industry which is based on PLS method; take the system thinking as the instruction, construct the model of customer satisfaction in the technical project service industry which take the customer satisfaction degree as endogenous latent variables, and take the customer expected, the sensation quality and the sensation value as exogenous latent variable.

本文针对描述客户满意度与其影响因素间关系模型所面临的潜变量影响，结合偏最小二乘法与结构方程模型各自的特点，提出了基于PLS的结构方程模型应用在服务行业的问题；以系统思想为指导，构造了以客户满意度为内生潜变量，以客户期望、感知质量与感知价值为外生潜变量的技术工程服务行业客户满意度模型。

A new pricing approach to real option is thus proposed to transform the forecast intervals evaluated by experts into some normal fuzzy numbers with the lattice closeness degree introduced to construct weighted vectors. In this way the rationality of estimating the present value of expected cash flow payoff by using a normal fuzzy number is verified.

提出了将预期现金流收益现值的专家评估区间转化成正态模糊数并利用格贴近度构造权向量的一种新的实物期权定价方法，验证了利用正态模糊数估计现金流收益现值的合理性。

During the panorama generation,this paper gives an algorithm to construct a spherical panoramic image automatically based on obtaining the perfect parameters of mosaic,which has the characteristics of expeditiousness ...

在全景图的生成中，提出了一种基于拼合参数自动寻优的球面全景图生成方法。该方法具有拼合速度快、拼合效果好的特点。

All these things are not commonly noticed in looking at a table, because experience has taught us to construct the 'real' shape from the apparent shape, and the 'real' shape is what interests us as practical men.

在看一个桌子的时候，所有这些情况通常都未曾被人注意，因为经验已经教会了我们要从外表的形状构想"实在的"形状，而"实在的"形状才是我们作为实践者所感兴趣的东西。

It may be divided into two parts except for introduction and conclusion. Firstly, chapters 2,4,5,6 investigate and analyze the informal debt-collection practice in Southern China, and chapter 3 deals with the concept of self-help explanatorily. Secondly, chapters 7,8,9 explore self-help more generally and try to construct a general theory of self-help remedy.

除导论和结语外，全文可分为二大部分：第二、四、五、六章系对华南一个民间收债个案的调查与分析，其中插入第三章私力救济的概念；第七、八、九章虽未完全脱离个案，但力图放宽视野，更一般地分析私力救济现象和进行理论建构。

The objective of this paper is to estimate the parameters of generalized exponential family ARMA models and construct the confidence intervals of the parameters. In this paper we divided the problem into continuous type situation and discrete type situation.

本文针对广义指数族ARMA模型，分为连续型指数族和离散型指数族两种情况讨论模型的参数估计，并运用不同的Bootstrap方法构造参数的置信区间和置信带。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。