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Restrictive changes of TCR Vp repertoire and clonal expanded T cells could be found in peripheral blood T cells from patients with T-ALL. However, the clonal expanded T cells' property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. It may play a certain role on detection of minimal residual disease and design of anti-leukemia idiotypic vaccine. The dominant utilization of TCR VjJ repertoire could be found in peripheral blood T cells from patients with B-ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia-associated antigen. The clonal expansive tendency of T cells in V021 and VP23 subfamilies was rather obvious, which may be correlated with certain malignant B-cell clone. The CDR3 sequences of monoclonal expansive T cell in T cell strains were different, which was the base of developing DNA vaccine.

T-ALL患者外周血T细胞的TCR Vβ谱系出现限制性改变，均可检测到克隆性增殖T细胞，尚需进一步鉴定其性质(肿瘤性或抗原特异性增殖)，对于研究微小残留病变检测和设计抗白血病独特型疫苗均有一定的意义。B-ALL病人外周血T细胞的TCR Vβ谱系呈现优势利用的特点，并存在克隆性增殖的T细胞，这可能是机体对白血病相关抗原产生的特异性免疫反应；Vβ21和Vβ23亚家族T细胞发生克隆增殖改变的趋向性比较明显，可能与B-ALL相关抗原刺激有关。T细胞株中检测到的肿瘤克隆其CDR3序列具有高度的特异性，是构建DNA疫苗的基础。

In order to disclose the adaptive strategies and capacity of Potaninia mongolica Maxim, acquired during its evolution history, the whole response system, which was composed of individual morphology plasticity response, organs anatomy structure response, physiological function response of anti-arid and anti-hotness, activities of protective enzymes response, endogenous hormone adjustment response, sexual reproduction strategies response of seeding, dispreading, sprouting and renewing, asexual reproduction strategies response of clonal growth patterns, clonal growth architectures, clonal growth architectures plasticity, heterogeneous resources utilization strategies, endogenous hormone distribution in clonal organs, foraging behavior, risk-spreading, and resource sharing, individual density allocation patterns response, niche differentiation response, species connection response, allelopathy response and biodiversity components response of the plant was profoundly explored in this paper.The studies come to at least four important results:(1) Taking the sensitive response and evading strategy to adapt to environment stress, Potaninia mongolica Maxim is a successful species has lived through the long-term evolution. Nevertheless, it is narrow climate and soil niche and characteristic of vegetation reproduction which has made the plant a rare and endangered species.

本论文通过对绵刺环境胁迫下个体形态可塑性响应、器官解剖结构响应、抗旱性和抗热性生理功能响应、保护酶系统激活响应、内源激素调控响应，有性繁殖对策中结实、扩散、萌发、更新等生活史过程响应，无性繁殖对策中克隆生长格局响应、克隆生长构型响应、克隆生长构型可塑性响应、克隆生长构型与资源异质性响应、内源激素调控克隆生长构型响应、克隆器官结构与功能响应、觅食行为响应、风险分摊响应、资源共享响应，种群中格局配置响应、生态位分异响应，群落中种间关联、异株克生响应、生物多样性组成响应等内容进行研究，揭示绵刺在进化过程中的适应对策和适应能力，为绵刺保护提供理论依据。

Both the interactions of haplotype vs. food (R0: P=0.038; rm: P=0.027) and that of haplotype vs. temperature (R0: P=0.006; rm: P=0.000) had obvious effect on growth reponses of the clonal groups. Moreover, there was significant interaction of haplotype vs. temperature (lorica length: P=0.033; lorica width: P=0.000) and haplotype vs. temperature (lorica length: P=0.027; lorica width: P=0.000) on body size of the clonal group.

生命表实验结果显示，单倍型与食物浓度的交互作用（R0：P=0.038；rm：P=0.027）以及单倍型与温度的交互作用（R0：P=0.006；rm：P=0.000）对种群增长均有显著影响，这4个单倍型克隆群对环境条件具有不同的偏好，并且各个克隆群对于环境变化有不同的生殖反应。

So it couldn't fit dynamic network environments soundly. After investigating of negative selection, affinity mature and memory detector evolution mechanisms, it is Embedded Negative Selection Operator Clonal Selection Algorithm presented combined with negative selection, clonal selection and memory detector gene library evolution.

本文结合网络入侵检测的上述问题，深入研究了否定选择、亲和力成熟过程以及免疫记忆机制等，以现有克隆选择算法为主体，将否定选择、克隆选择、记忆检测器基因库方法融合进来，提出嵌入否定选择的克隆选择算法(Embeded Negative Selection Operator Clonal Selection Algorithm， ENCSA)。

A novel Multi-Objective Immune Algorithm based on Memory Clonal Selection is proposed in this paper by introducing the mechanism of clonal selection in organismal immune system.

借鉴生物免疫原理中克隆选择机理，设计了一种基于记忆克隆选择的多目标免疫算法。

And then the clonal selection algorithm using this clonal operator is discussed.

并对应用此算子的克隆选择算法进行了讨论。

Through analyzing static clonal selection algorithm, this thesis proposes a multilayer dynamic clonal selection algorithm.

本文通过对静态克隆选择算法的分析提出了一个多层动态克隆选择算法。

The results showed that the clonal reproduction was preponderant, but it has still a high genetic diversity (Ho=0.7148). At the same time, Corylus heterophylla Fisch genetic individual was found. A single cluster was basically a genetic individual; adjacent clusters were basically the same genotype and clonal reproduction has larger percentage.

结果表明：平榛虽然克隆繁殖占有优势，但仍具有较高的遗传多样性(Ho=0.7148)；同时确定了平榛的遗传个体，同1株丛内个体基本上为1个独立的遗传个体，相邻的株丛个体基因型基本一致，克隆繁殖比率较大。

To investigate the clonal rearrangements of immunoglobulin heavy chain gene and T-cell receptor γ gene in peripheral blood cells of patients with hemapoietic malignancies and their significance, the clonal rearrangements of IgH and TCRγ genes were detected by polymerase chain reaction in peripheral blood cells of patients with hemapoietic malignancies, including 32 cases of non-Hodgkin lymphoma, 18 cases of acute myeloid leukemia, 24 cases of multiple myeloma, 8 cases of acute lymphoblastic leukemia and 6 cases of chronic lymphoblastic leukemia.

检测各种血液系统肿瘤患者外周血细胞免疫球蛋白重链基因和T细胞受体γ基因克隆性重排并探讨其意义。通过多聚酶链式反应方法检测32例非霍奇金淋巴瘤、18例急性髓性白血病、24例多发性骨髓瘤、8例急性淋巴细胞白血病及6例慢性淋巴细胞白血病患者外周血细胞IgH及TCRγ克隆性基因重排。

Based on the clonal selection principle and idiotypic immune network theory of biological immune system,a dynamic clonal adjustment strategyis proposed.

基于生物免疫的克隆选择和独特型免疫网络机理，提出了动态克隆调节策略。

It has to be computed by the host for the actual clock rate.

主控制器可以用来计算实际的时钟速率。

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