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The results showed that:(1) The relative weight gain, special growth rate, feed conversion rate, feed protein efficiency and protein deposition rate were improved significantly (P.05) by supplementing compound acidifier in common carp diet.(2) The protease activities in the liver pancreas, fore and middle intestines increased significantly (P.05) by supplementing 0.3% compound acidifier. The activities of amylase and lipase were improved after the supplementation, but not at significant level.(3) Supplementation of compound acidifier in common carp diet did not significantly affect the fish body protein, lipid, ash, moisture contents, coefficient of fatness and viscerosomatic index.

结果表明：1添加复合酸化剂显著(P.05)提高鲤鱼的相对增重率、特定生长率、饵料转化效率、饲料蛋白质效率以及饲料蛋白质沉积率等生长性能指标；2添加质量分数0.3%的复合酸化剂时，鲤鱼肝胰脏、前肠和中肠的蛋白酶活性显著提高(P.05)，酸化剂组的淀粉酶和脂肪酶活性有升高的趋势，但与对照组差异不显著；3鱼体的粗蛋白质、粗脂肪、粗灰分、水分、肥满度及内脏比等品质指标在各组间差异不显著。

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示，银鲫pou2基因有母源转录本，其合子基因在高囊胚期强烈表达，在50%下包期和90%下包期也有高量的转录本，但在100%下包期表达量急剧降低，至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

Genetic diversity and phylogenetic relationship in goldfish were examined using mitochondrial D-Loop partial sequences at eight representative varieties of goldfishes and wild crucian carp. The eight representative varieties were Red Common Goldfish, Red Fantail, White Oranda with Red Carp, White Pearlscale, Red Tigerhead, Black Moor, Red Moor with Butterfly Tail and Red Bubble-eye with Dorsal Fin. 699 base-pair nucleotide sequences of Mitochondrial D-Loop were examined and analyzed for genetic polymorphism, Sequences data showed that all 129 sequences were grouped into 17 haplotypes. Nine representative varieties shared same haplotype. Five representative varieties of goldfishes had haplotypes respectively.

采用PCR技术扩增了红草金鱼，红文鱼，红顶白高头，白珍珠，红狮头，红龙睛蝶尾，墨龙睛，红水泡八个品种金鱼和野生鲫线粒体DNA的D-Loop区部分序列，PCR产物经纯化、测序、同源序列比对后获得长度为699bp的核苷酸序列，在129个样本中共检测到了17个单倍型，9个品种共享1种单倍型，5个品种的金鱼(BM， MB， WR， RF， RC)检测到各自独有的单倍型。

After 2 months, the samples of skin, scale, head and fm ray of the carps were selected for the subsequent analysis of determining their total carotenoid contents and the absorbance values, so the above solutions were evaporated to dryness, and then were measured by the thin layer chromatography. The results show that the spectrum of total pigment of koi carp had two absorption peaks in visible light (454 nm and 470 nm). The growth rate, weight gain and specific growth rate of red-white of koi carp fed with 1.0 g/kg β-carotene tended to be obviously higher than the control group (P.05), while the FCR of groups fed with 1.0~2.5 g/kg β-carotene was significantly lower than the control group (P.05). The colouring of red-white and red region in koi carp was the best at 1.5~2.0 g/kg β-carotene in diet, while the n-carotene added in the diets did not have any effects on total carotenoid concentration of skin/scale of white region. The n-carotene mainly deposited in skin and fin ray. And it mainly transformed into astaxanthin in koi carp.

结果表明，红白锦鲤总色素光谱在可见光区（454nm和470nm）有两个吸收峰，β-胡萝卜素在1.0g/kg组的增长率、增重率、特定增长率显著高于对照组(P.05)，1.0~2.5g/kg各组的饵料系数均显著低于对照组(P.05)；对于鲤的红白和红色区域，β-胡萝卜素的添加量为1.5~2.0g/kg时，对红白锦鲤体色的增色效果最好：β-胡萝卜素对红白锦鲤白色区域总类胡萝卜素的含量影响很小；β-胡萝卜素在红白锦鲤体内的主要沉积部位是皮肤和鳍条；β-胡萝卜素在红白锦鲤体内主要转化为虾青素。

Result succinylation modified, trypsin hydrolysis, SDS and β-mercaptoethanol treatment greatly reduced lectin from gill of bighead carp; dimethyl sulfoxide, NaCl, tri fluoroacetic acid at a given treated concentration had no effect on its activity; lectin from gill of bighead carp agglutinated Haenatcoccus pluvialis, vibrio harveyi and Vibrioauguil larum specifically.

结果］琥珀酰化修饰、胰蛋白酶水解、SDS及β-巯基乙醇处理均使鳙鳃凝集素活性大大降低；在设定的处理浓度下，二甲基亚砜、NaCl及三氟乙酸对其活性没有影响；鳙鳃凝集素能专一地凝集雨生红球藻及哈维氏弧菌和鳗弧菌。

No fragments were amplified from some loci of EST-SSRs, which may suggest the loss of these loci in mirror carp genome or sequence divergence at the primer binding sites. These null alleles may result from selection because functional genes are under more selection pressure than non-encoding loci. Overall, population genetic variation is high for each of the five mirror carp, and the differentiations are also significant among populations.

3在与功能基因相关的多个微卫星基因座位上，扩增产物呈现不同程度的缺失现象，这些无效等位基因的产生可能与结构基因在育种中受到人工选择的影响较大有关。

Carp were fed with medicated diet added with Chinese nutgall at the weight rate of 0.5% or 1.0%, by examining the indices of liver and blood , the effect of Chinese nutgall to carp bacterial septicemia was studied .

以0.5%和1.0%五倍子药饵饲喂鲤鱼，通过肝脏和血液指标的测定，研究五倍子对鲤鱼细菌性败血症的药效。

The deduced amino-acid sequence of the TcGH had 210aa,including a putative signal peptide (22aa) located at its N-terminal. The homology of the TcGH amino-acid with relative species grass carp,black carp,bighead carp,carp,zebra fish,etc.

结果序列分析表明：唐鱼GH cDNA的5 非编码区为64 bp，3 非编码区为448 bp，开放阅读框633 bp，共编码210个氨基酸，包括22个氨基酸的信号肽和188个氨基酸的成熟肽。

The toxical effects of nonylphenol ethoxylates and nonyiphenol were studied with EROD in liver of carp as biomarker under external condition. These two tested substances all had activation on EROD enzyme in liver of carp. Under definite range of concentrations, with the affecting rule of inhibiting at high concentration and inducing at low concentration.

在离体条件下，以鲤鱼肝脏EROD酶为生物标志物研究了壬基酚聚氧乙烯醚及壬基酚的毒性效应结果表明，在一定的浓度范围内，2种受试物对鲤鱼肝脏EROD酶均有激活作用受试物浓度对EROD酶的活性的影响规律为高浓度时抑制，低浓度时诱导。

The release to the rulers of China's freshwater fish species,"the four fish"-based, grass carp to control aquatic plants, silver carp and bighead carp can be swallowed water plankton, herring at the bottom of the water body may limit the habitat of animals, so that can not only improve the the aquatic environment, but also can improve water fish production.

这次放流以我国淡水鱼类的当家品种&四大家鱼&为主，草鱼可以控制水草，鲢鱼和鳙鱼可以吞食水体中的浮游生物，青鱼可以限制水体底部的栖息动物，这样不仅能改善水生态环境，而且可以提高水体鱼的产量。

He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州，并在2005年伊瓦尔街，公寓生活。

Droperidol （ potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道，可能是 QT 间期延长的机制。