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Above stage- and cell-specific expression patterns of PKB/Akt, FoxO1, PDE4, inhibin α-subunit, nNOS, sGC α and β subunits suggest that these proteins might play important roles in follicular development, atresia and luteinization in the Chinese Holstein Cow.

这七种蛋白的阶段和细胞特异性表达表明它们与中国荷斯坦卵巢卵泡的发育、闭锁和黄体化过程有着密切的关系。

In neonates and infants, choledochal cysts are congenital, may be associated with distal biliary atresia and typically presents with cholestatic jaundice and acholic stools characteristic of biliary obstruction.

新生儿和婴儿期之胆道囊肿是先天性的且常合并远端的胆道闭锁,所以常以胆道阻塞的特徵如胆汁郁滞和灰白色大便为主要徵候。

There are also many pediatric liver disease, including biliary atresia, alpha-1 antitrypsin deficiency, alagille syndrome, and progressive familial intrahepatic cholestasis, to name but a few.

还有许多儿童肝脏疾病,包括胆道闭锁、阿尔法-1抗缺失,郁积综合症家族性肝内胆汁和进步,是名副其实的。

E. staining and other techniques were used to elucidate the pattern of follicular development, atresia, changes in follicle number on the ovarian surface, proportions of apoptotic granulosa cells in follicles of different sizes and morphological changes of atretic follicles, as well as changes in serum estradiel and progesterone levels at different stages of the porcine estrous cycle.

为探讨猪发情周期不同时期卵泡发育和闭锁的规律及机理,本实验通过TUNEL原位标记、H.E。染色以及放射免疫测定等手段研究了猪发情周期不同阶段卵巢表面各类卵泡数量的变化、各类卵泡中颗粒细胞的凋亡比例、闭锁卵泡的形态变化以及发情周期各阶段血清孕酮及雌二醇水平的变化等问题。

The BMP15 protein played a certain role in all the stages of follicular development and had a relationship with the luteal dissolution mechanism; In the atretic follicle, the low expression of BMP15 protein maybe caused by inhibition from the other factors which lead to connected modification of follicular atresia.

结论]BMP15参与了兔卵泡发育全过程,并且与黄体溶解机制有关;而在闭锁卵泡中,其低水平的表达可能是由其他因子所抑制,进而共同调节了卵泡的闭锁。

The vast majority of follicles undergo a degenerative process in reproductive life. Apoptotic cell death is the fundamental mechanism in response to follicular atresia. Moreover, the apoptosis of granulose cells is implicated in directly mediating atretic process. Multiple studies have confirmed that the apoptotic process of GCs is not only related to apoptotic genes, but triggered by cytokines related to reproduction and Caspases family.

中文题名几种生殖激素对体外培养的牦牛卵泡颗粒细胞凋亡的影响副题名外文题名 Effects of several reproductive hormones on apoptosis of ovarian granulose cells of yaks in vitro 论文作者杨世华导师余四九教授学科专业临床兽医学研究领域\研究方向学位级别博士学位授予单位甘肃农业大学学位授予日期2002 论文页码总数108页关键词牛类固醇激素分泌卵泡颗粒细胞培养牦牛生殖激素馆藏号BSLW /2003 /S823 /1 卵泡闭锁是哺乳动物卵巢中极为普遍的自然现象。

We observed changes of GV chromatin configurations in porcine, caprine and bovine oocytes from follicles of different sizes and degrees of atresia and during maturation in vivo and in vitro, using a new method for preparation of GV samples.

本项目发明了一种特殊的卵母细胞生发泡标本制备技术,能克服脂肪滴等对生发泡染色质构型观察的阻碍;经Hoechst或CMA3染色后可清楚地观察染色质构型的变化。

The new GV preparation method we developed allowed the best visualization of chromatin distribution after Hoechst or CMA3 staining. Our results showed that GV chromatin configurations are different among different species, and thus, while chromatin condensed into a ring around the nucleolus in porcine and bovine oocytes, no such a ring was observed in caprine oocytes; Porcine oocytes were synchronized at GV1, goat oocytes at GV3n and bovine oocytes at GVf configurations before GVBD occurred, indicating that these configurations are progressive towards final maturation rather than atresia; Changes of chromatin configurations were associated with gene activity of transcription; GV chromatin configurations were affected by the environmental changes such as serum starvation and elevated temperature.

应用该技术对猪、山羊和牛不同大小、不同健康程度卵泡及体内和体外成熟过程中卵母细胞染色质构型的变化发现:1)不同动物卵母细胞染色质构型不同;猪和牛卵母细胞染色质围绕核仁凝集形成环,但山羊卵则没有此环。2)卵母细胞在GVBD之前都同步在某一染色质构型;猪同步在GV1,山羊GV3n,牛GVf,说明这些染色质构型代表着进行性变化。3)染色质构型的变化反映基因转录活动的变化。4)染色质构型受环境变化的影响。

AIM: To explore the relationship between human cytomegalovirus infection and infantile hepatitis syndrome, biliary atresia, and choledochal cyst.

目的:探讨人巨细胞病毒感染与婴儿肝炎综合征、胆道闭锁、胆总管囊肿的关系。

HCMV infection is associated with IHS and biliary atresia, but not with choledochal cyst.

IHS及胆道闭锁的发病与HCMV感染有关,胆总管囊肿的发病与HCMV感染无明显相关性。

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