查询词典 assay

The Thiobarbituric acid-reactive substances assay is a sensitivity HPLC assay that measures a chromogen that is produced by the reaction of thiobarbituric acid with malondialdehyde, which is an endproduct of lipid peroxidation.

硫巴比妥酸反应物分析，利用HPLC的灵敏特性分析硫巴比妥酸与丙二醛反应所产生的发色体，其为内生性脂质过氧化物。

The assay of DPPH free radical clean test best is Cissus pteroclada, it has over 95%, in anti-liver lipid peroxidation assay, the best is Ampelopsis cantoniensis, it has over 95% too, and Ampelopsis Japonica has well effect on killing human breast cancer cells MCF-7 but have no effect about anti-oxidation.

抗肝脂质过氧化试验中以广东山葡萄最好，其抑制率有95%以上。白蘝对於抗氧化虽然无特别效用，但在人类乳癌细胞株MCF-7毒杀试验上有很好的成效其抑制细胞生长率为95%以上。

All of the coefficient of variation of intra-assay and inter-assay for quantitative detection were less than 2%.

定量检测重复性试验结果，批内和批间的变异系数均小于2％。

Methods: We used virus proliferation assay cell viability assay to evaluate the proliferation and cytolysis selectivity of CNHK500. And we used Western-blot to confirm the expression of adenovirus CNHK500 E1A and E1B in cancer and normal cells.

行病毒增殖实验和细胞生长抑制实验，验证CNHK500选择性复制和杀伤能力；Western blot检测腺病毒E1A和E1B在细胞中的表达。

Methods: Virus proliferation assay, cell viability assay and Western blot were performed to assess the selective replication and cytolysis of CNHK500 in telomerase positive liver cancer cells Hep3B, HepGⅡ, SMMC7721 and in normal cells.

方法利用病毒增殖实验、细胞活力实验、蛋白印迹分析来检测增殖病毒CNHK500在端粒酶阳性的肝癌细胞株HepGⅡ、Hep3B、SMMC7721及正常细胞中选择性增殖和溶解细胞的特性。

Methods: The telomerase activity in breast cancer cells and MRC-5 cell line were detected semi-quantitively by TRAP-ELISA assay. Virus proliferation as- say and cell viability assay were used to evaluate the proliferation and cytolysis selectivity of CNHK300, and the results were compared with those of wtAd5. The breast cancer cells were treated with CNHK300 combining paclitaxel to evaluate the killing effect of the combined regimen.

用TRAP-ELISA方法检测乳腺癌细胞株(MDA-MB-453、SK-BR-3)和正常成纤维细胞株MRC-5的端粒酶活性；进行病毒增殖实验和细胞生长抑制实验，并与野生型腺病毒wtAd5进行对比，验证CNHK300选择性复制和选择性杀伤能力；用细胞生长抑制实验进一步考察CNHK300与化疗药物紫杉醇联合应用对乳腺癌细胞的杀伤作用；用Western blot检测腺病毒E1A在细胞中的表达。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Now the research on HPL focus on dicotyledon, and the research on HPL of monocotyledon still havent been reported. In this paper, the research on cloning of rice HPL and enzyme activity assay were carried out and several result were obtained.1. Enzyme activity assay of rice HPLBased on the method of measure HPL Enzyme activity in other plants, the means on preparation of hydroperoxides and measurement rice HPL activity were set up. The 9-HPL arid 13-HPL activity of several rice varieties with different storability and different dormancy were tested.

本试验建立了水稻的HPL酶活性测定体系，测定了水稻萌发过程中的HPL酶活性，并进行了水稻HPL基因的克隆及表达研究，结果如下： 1、水稻HPL的酶活性测定在参考其它植物HPL酶活性测定方法的基础上，建立了水稻的HPL底物发生体系和酶活性测定体系，并测定了不同贮藏性水稻品种萌发过程中的种胚9-HPL、13-HPL活性和不同休眠性水稻品种种子的9-HPL、13-HPL酶活性。

MUG single tube quantitative assay method was introduced to Escherichia coli assay for bacterial suspensions,natural seawater,and fishery product samples.The detected data was analyzed and compared with multiple tube fermentation method.

采用MUG单管定量检测法对实验室制备的菌悬液、天然海水、水产品进行定量检测，并与大肠埃希氏菌多管发酵法的检测结果进行比对分析。

A multiplex PCR assay for detection of Escherichia coli O157:H7 was developed by using 3 sets of primers that specifically amplify segments of the rfbE、fliC and eaeA genes. The target genes fragment of the PCR assay were 291 bp, 625 bp and 368 bp, respectively. Analysis of 30 strains demonstrated that this PCR system was specific.

以编码大肠杆菌O157抗原的rfbE基因、编码H7抗原的fliC基因以及编码毒力因子的eaeA基因为靶基因，选择3对引物，建立并优化了检测大肠杆菌O157：H7的多重PCR体系，扩增产物分别为291 bp、625 bp、368 bp，采用30株细菌验证了该多重PCR具有特异性。

I am sure after a few weeks of adaptation and familiarization I will have no difficulty in functioning on a daily basis in an English speaking society or in participating fully in graduate studies.

在大学的三年的学习生活不仅给予我自身的充实，更重要的是磨练和培养了我较强的自觉能力和严谨，踏实的作风以及不怕困难的精神，对未来充满了信心！

As the first volcanoes erupted, one of the gases that bellowed out was steam.

在第一批火山喷发时，喷出的气体之一就是水蒸汽。