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Isolation, cultivation and identification of human embryonic NSC from cerebral cortex of 7-12 week abortus. By using flow cytometry and MTT assay, the effects of various concentration of TSPG and TSPG cooperating with cytokines in NSC culture media for 3 days on proliferation of human embryonic NSC has studied. By employing immunocytochemistry assay of the expression of tyrosine hydroxylase, the effect of different dilution of TSPG and TSPG cooperating with IL-1 on induced differentiation of human embryonic NSC into dopaminergic neuron has researched.

从7～12周人工流产胚胎脑组织中分离培养人胚胎NSC，免疫细胞化学鉴定NSC特异性nestin抗原表达，在NSC的培养体系中加入TSPG，采用流式细胞术和MTT法检测不同浓度TSPG以及TSPG与EGF，bFGF联合应用对人胚胎NSC增殖的影响；经免疫细胞化学检测培养细胞酪氨酸羟化酶表达，分析不同浓度TSPG以及TSPG与IL-1联合应用对NSC定向诱导为DA能神经元的影响。

To study the changes of plasma endotoxin NO and hemorheology in experimental cirrhotic rats and their correlation,we have used hemorheology analysis system and the means of limulus amoebocyte lystata assay Griess assay to detect changes of plasma endotoxin NO and hemorheological parameter in rats at 3 6 9 weeks after CCl4 administration.

为探讨肝硬化中血浆内毒素、NO水平的变化及血液流变学特性的改变及其相互关系，我们通过用四氯化碳(carbon tetrachloride，CCl_4)来诱导大鼠肝硬化模型，采用鲎试剂动态浊度法、镉还原比色法、血液流变学分析仪，分别检测了大鼠肝硬化演变过程中早期(诱导第3周末)、中期(诱导第6周末)、晚期(诱导第9周末)的血浆内毒素、NO水平及血液流变学各参数。

Keywords Sheep;Goat;Anthelmintic resistance;PCR-SSCP;PCR-RFLP;Faecal egg count reduction test;Egg hatch assay;Larval development assay

绵羊； 山羊；驱虫药抗药性； PCR-SSCP； PCR-RFLP；粪便虫卵减少试验；虫卵孵化试验；幼虫发育试验

Upon the optimized Agrobacterum-mediated transformation systems mentioned above, the herbicide-resistant gene bar has proved to be successfully incorporated into genomes of the Cynodon dactylon after PCR assay of hpt gene and bar gene, GUS histochemical assay and hpt resistance evaluation.

经潮霉素抗性基因hpt和除草剂抗性基因bar的PCR分析、GUS组织化学染色和潮霉素抗性鉴定，证实除草剂抗性基因bar已成功整合到狗牙根基因组。

The testing method contained fibrin plate assay, direct and indirect chromogenic assay, SDS-PAGE fibrin autography, and visual exhibition of the complexes between rPAI-1 and t-PA or low molecular weight u-PA on SDSPAGE.

所使用的方法包括：纤维蛋白平板法，直接和间接的显色底物法，SDS-PAGE纤维蛋白自显影法，以及用SDS-PAGE直接显示rPAI-1与t-PA和低分子量u-PA形成的复合物。

Methods SOS/umu assay and the ethidium bromide flurescence assay and the single cell gel electrophoresis assay were applied respectively to detect the DNA damage and DNA cross - links and DNA single strand breaks after exposure to the particle extracts of air pollutants in TSCDG.

方法应用SOS/umu试验，大鼠肺Ⅱ型细胞的DNA交联试验和单细胞凝胶电泳试验，检测危险品货运站空气污染物对DNA的损伤情况。

IL-6,TNF-α levels in sera were determined by means of enzyme linkage immuno-sorption assay, 3H-TdR release assay was used to measure NK cell activity, and APAAP bridge enzyme label assay was used to type subpoppulation of T lymphocytes.

用酶联免疫吸附法检测血清IL-6、TNF-α浓度，3H-TdR掺入法检测NK细胞活性，APAAP桥联酶标法进行淋巴细胞亚群分型。

Methods Pull-Down assay in vitro, Far-Western Blot assay and Co-Immunopreci-pitation assay in vivo were used to detect the binding of HBX protien to TF ⅡB.

应用体外蛋白与蛋白结合试验(Pull-Down试验)和Far-Western Blot试验，以及细胞内联合免疫沉淀试验。

RESULTS: Wound healing rate, wound healing time, histopathology analysis, quantity assay of macrophage, determination of hydroxyproline, proliferation of cell, assay of DNA contents and circle of cells, level of transforming growth factor-alpha, levels of interleukin-1, interleukin-6 and tumor necrosis factor, assay of keratinocyte collagenase-1, level of fibroblast growth factor receptor-1, level of monocyte chemoattractant protein-1 and level of keratinocyte plasminogen activator inhabitor type 2 were selected as the evaluation criteria of wound healing.

结果 筛选了创面愈合率、创面愈合时间、组织病理学分析、巨噬细胞定量分析、羟脯氨酸含量测定、细胞增殖情况、细胞DNA含量和细胞周期分析、转化生长因子-α水平、白细胞介素-1、白细胞介素-6和肿瘤坏死因子水平、角质细胞胶原酶-1含量测定、成纤维细胞生长因子受体-1水平、单核细胞化学诱导蛋白-1水平和角质细胞纤溶酶原活化抑制剂-2水平等十三种创面愈合评价指标。

The effect of proliferation,invasion and tube formation on SGC7901 cells were detected by colone formation assay,invasion assay and tube formation assay respectively in vitro.

Maniotis AJ等发现具有高度侵犯性的黑色素瘤组织可以形成模拟的血管通道并称之为血管生成拟态(vasculogenic mimicry，VM)[1]。

Further more, the simplified analysis method on stressed-skin effect of corrugated steel sheet is also discussed in detail.

此外还比较详细地探讨了蒙皮效应的简化分析方法。

If I had known her telephone number,I would have called her.

要是我知道她的电话号码，我就打电话给她了。