查询词典 amplification

We investigated the impact of polysomy 17 on HER-2 testing and studied its clinicopathologic significance in relation to HER2 gene amplification.

该研究的FISH结果显示，44例患者的HER2绝对拷贝数达可疑阳性，但如果结合17号染色体多体因素(HER2/Chr17)，这些患者均为HER2阴性。

On its own, polysomy 17 was not associated with HER-2 overexpression on IHC or increased HER2 mRNA levels by RT-PCR. Moreover, and in contrast with HER2 gene amplification, polysomy 17 was not associated with high tumor grade, hormone receptor negativity, or reduced disease-free survival.Conclusion: Polysomy 17 affects HER-2 testing in breast cancer and is a major cause of equivocal results by FISH.

不过，在J Clin Oncol同期配发的一篇述评中，美国波士顿大学医学中心的罗森伯格指出，当务之急不是直接进行这样的研究，而是应该对已有研究的IHC、FISH和有效率数据进行回顾或再分析，以明确是否无HER2扩增的17号染色体多体肿瘤被归入不同类别(HER2阴性、阳性)，并由此导致关于HER2靶向治疗疗效的结果不一致。

We show that tumors displaying polysomy 17 in the absence of HER2gene amplification resemble more HER-2–negative than HER-2–positive tumors. These findings highlight the need for clinical trials to investigative whether polysomy 17 tumors benefit from HER-2–targeted therapy.

在他看来，若要进一步改进乳腺癌靶向治疗的患者选择和转归，遗传和药理学基础研究、已有研究数据再评价以及肿瘤早期HER2变异研究需要齐头并进。

With the current mode logic structure, the input buffer and the predriver circuit have the capability of transmission and amplification of high speed data. By employing MOS-HBT cascode structure as the output stage, the laser diode driver exhibits very high speed and efficiency working at the 10 Gb/s data rate.

输入缓冲、驱动放大电路采用电流模电路，满足高速数据传输和放大的能力，输出级电路结构采用新型的MOS-HBT共源共栅结构可以降低米勒效应减小输入电容，从而使激光驱动芯片工作在10Gb/s时也能达到良好的性能。

Amplification of n sequence by PCR can be used to detect HD gene presymptomatically and prenatally in HD families and to discriminate clinical cases with untypical features from HD.

应用PCR技术直接检测动态突变的重复序列，可为这些疾病的临床诊断和鉴别诊断提供有力的依据。

And the amplification procedure was as follows: preparatorily denaturing for 3 min at 94℃, denaturing for 30 sec at 94℃, annealing for 30 sec at 37℃, elongating for 90 sec at 72℃, 41 cycles, and elongating for 7 min at 72℃.

扩增程序为：94℃预变性3 min，然后于94℃变性30 s，37℃复性30 s，72℃延伸90 s，循环41次，最后于72℃延伸7 min。

Due to severe amplification by his mutations, this power can psychically reshape the battlefield perceived by all sides for a short duration.

而且随着他的变异加深，这项能力也得到了加强，现在&地狱&能力可以在一段时间内重塑战场上所有人对战况的感知意思就是所有部队的士气好坏都要看他的意思。。

Based on the simplification expression principles and amplification classification of urban models, this paper puts forwards a fast model-construction method with symbol matching and polygons triangulation respectively to punctate, linear and polygonal objects.

基于3DUGIS中景观模型的表达原则与分类，分别针对抽象的点、线、面状对象提出符号匹配和三角剖分的批量三维模型构建方法。

MethodUsing the canis MC4R DNA as template,the specific primers were designed. After PCR amplification,product was cloned into pGEX-4T-1 vector by LP Recco PCR cloning technique,the recombinant pGEX-4T-1-cMC4R was transferred to E.

方法以Beagle犬基因组DNA为模板，经PCR技术扩增目的片段，利用LP Recco PCR克隆技术将目的片段直接重组到原核表达载体pGEX-4T-1上，转化到E.coli DH5α，筛选阳性克隆，BamHI、XhoI酶切鉴定，DNA 测序检测插入序列的正确性。

The DNA of Yak PrP expressed plasmid extracted from the recombinant expressed bacterium was identified by PCR amplification and two-ezyme digestion. The expressed product was obtained from the recombinant bacterium inducted under the optimized expressed condition (37℃, 1 mmol/L IPTG, 6 h), and it was determined by SDS-PAGE and Western blotting. The GST-BoPrP(23—242) fusion proteins were collected by two ways: the first is to purify and renature from the inclusion bodies; the second is to separate by SDS-PAGE.

将保存的含有牦牛PrP基因重组表达菌提取质粒DNA，进行PCR扩增和双酶切鉴定，并在优化诱导表达条件(37℃，1mmol/L IPTG，6h)下，获得的表达产物进行SDS-PAGE和Western blotting检测；将鉴定之后的重组表达菌诱导表达后，通过两种方法回收GST-BoPrP(23～242)融合蛋白：其一，是从包涵体中提取和复性GST-BoPrP(23～242)融合蛋白；其二，是通过SDS-PAGE直接分离并纯化GST-BoPrP(23～242)融合蛋白。

It's bell can reach over a meter in diameter.

它的身体最大可以达到直径1米多。

Now they are overdue and I shall have to pay a fine.

现在他们过期了，我不得不付罚款。