查询词典 amplification

Rapid amplification of cDNA end:Retroverse transcription product of total RNA extracted from normal porcine tissue was used as the template,gene specific primers were designed and advantage 2 polymerase mix was used in PCR,of which using porcine genomic DNA as the template:forward primer was designed according to the acquired consensus region of human and pig FGL2 3′ sequences while reverse primer was designed from human FGL2 3′ end downstream sequence;TA cloning.

以猪正常小肠及心脏组织提取新鲜总RNA，反转录后作为模板，设计基因特异性引物，采用Advantage 2 聚合酶混合物进行PCR扩增；依据猪与人FGL2基因3′端已知同源序列设计PCR上游引物，以人FGL2基因3′末端序列设计下游引物，以猪基因组DNA为模板采用Advantage 2 聚合酶混合物进行PCR反应；PCR载体重组质粒DNA亚克隆扩增。

The cDNA (BplPAL1) encoding phenylalanine ammonia-lyase were cloned from Betula platyphylla by reverse transcription polymerase chain reaction and 5' and 3' rapid amplification of cDNA ends, which contains an open reading frame (2322 bp) encoding 773 amino acids. The amino acid sequences contain two functional domains of PAL-HAL and PAL, and the sequence of enzyme active site, which has 60%~73% of consistency with other five plant species and the consistency with Rhizophora mangle is the highest (73.1%).

利用RT-PCR和RACE技术从白桦中克隆了编码苯丙氨酸解氨酶的cDNA，其2322bp的ORF编码773个氨基酸，其推导的氨基酸序列包含PAL-HAL和PAL2个功能域以及酶活性中心序列GTITASGDLVPLSYIA，该序列同其它5种植物的序列一致性为60%~73%，其中与美洲红树最高为73.1%。

The results of GUS histochemical assays and PCR amplification demonstrated that the overall transformation rate of Roselle callus was 4%.

GUS活性组织化学检测和PCR扩增鉴定的结果表明，愈伤组织的转化率为4%。

Characteristic matrix method is used to analyse the spectral response of the Rugate filter approximately. Amplification using Rugate filter spectral compensation is analysed numerically.

由普通均匀多层膜的特征矩阵方法近似分析了设计出的Rugate滤波器的光谱响应，数值模拟了Rugate滤波器的光谱补偿效果。

In this study, SSR analysis was performed on five Secale species, four Triticum species, and a Triticale with branch-like using 102 pairs of microsatellite primers. A 387bp specific DNA fragment FZ387 (Accession No. EF179137), was obtained from Triticale with branched-like by primer Xgwm614; however no amplification result revealed in Secale.

利用102对微卫星引物对5份黑麦、4份普通小麦和1份分枝小黑麦进行SSR分析，引物Xgwm614能在分枝小黑麦中扩增出一个387bp的特异DNA片段（记为FZ387，GenBank登录号为EF179137），而黑麦未能扩增出。

Total RNA was isolated from the forth instar larvae of the armyworm,Mythimna separata Walker.An α and a β tubulin cDNA sequences were cloned by RT-PCR and rapid amplification of cDNA ends.

以黏虫4龄幼虫为材料提取总RNA，利用RT-PCR和cDNA末端快速扩增技术，分别扩增得到该虫的α和β微管蛋白基因的cDNA序列各1条。

Methods Bacterial culture, serological detection and DNA amplification were carried out simultaneously for each sample of 154 patients.

对154名社区获得性肺炎患者的标本进行细菌培养和血清学检测，并进行药物敏感性试验。

Serological detection and DNA amplification were carried out simultaneously for each sample of 154 patients.

建立了非典型病原体的多重PCR检测方法，多重PCR体系的敏感性：100copies/100μl。

By sampling echo signal demodulated and A/D transform, sampling value is transmitted to SCM for compare, division, calculation, and exporting gain code to control signal range; furthermore controlling the AGC circuit, realizing amplification control to echo signal, consequently stabilitate the signal range in a window for the later circuit.

通过对检波后的回波信号进行采样，经过A/D变换，采样值送入单片机内部进行比较、判别、计算，然后输出可控制信号幅度的增益码去控制AGC电路的受控级，实现对回波信号的增益控制，从而使得输出信号幅值稳定在一个窗口内，供后续电路处理。

Methods The PCR enhancers,like dimethyl sulphoxide,glycerol,betaine and betaine of different concentrations were put into the LD-PCR mixture,then the results of amplification were compared.

在LD-PCR反应体系中加入DMSO、甘油及不同浓度的甜菜碱进行LD-PCR扩增，比较扩增结果。

It's bell can reach over a meter in diameter.

它的身体最大可以达到直径1米多。

Now they are overdue and I shall have to pay a fine.

现在他们过期了，我不得不付罚款。