查询词典 amplification factor

The amplification failure rate of the single cell 5-plex nest PCR is significantly associated with the difference of the methods of cell lysed. Using appropriate method to lyse cell can improve the accuracy of single cell PCR.

结果表明单细胞PCR模板制备与PCR扩增失败有密切关系，选用合适的细胞裂解方法可提高单细胞PCR的准确性。

Finally designs has the tonality control circuit the audio frequency amplification electric circuit to have very well chooses the frequency function, with pronunciation magnifying power.

最后设计出来的带有音调控制电路的音频放大电路具有很好的选频功能，和语音放大能力。

The multiple scattering which doesn＇t form loop can increase the mean free path, thus enhancing light amplification , and making laser in various directions.

不构成回路的多重光散射增加了激光在增益介质中的路程，使激光增强，并使激光沿各个方向发射。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

At last, through the analysis of the T-stress, it is concluded that the T-stress plays an important role for the oriented microcrack cases. It is necessary to consider the effect of the T-stress in determining the shielding or amplification effect of the oriented microcracks.

最后，本文采用离散模型研究了各向异性材料主微裂纹干涉问题中T应力对主裂尖应力强度因子的影响：T应力对主裂尖应力强度因子的影响是不能被忽视的，这种影响不但与微裂纹的几何分布有关，而且还与T应力的大小和符号有关。

Laser capture microdissection combined with RNA linear amplification in vitro can be successfully applied to obtain pure objective cells for research. The integrity of the amplified RNA is good and can be employed in further research.

结论LCM结合RNA体外线性扩增技术能成功地获取较为单一的研究目的细胞，扩增后RNA完整性较好，能用于进一步研究中。

METHODS Microdissection technique with micromanipulator under microscope, as well as subsequent DNA extraction and polymerase chain reaction amplification methods, was employed.

通过光学显微镜直视下用显微操纵系统进行显微切割技术结合DNA提取和PCR方法，对在组织切片上获取病毒核内包涵体的来源进行分子生物学鉴定。

Based on the analysis of potential vorticity and elements on isentropic surface about the typhoon 9608 and its heavy rain outside, the study is to show the PV structure and the interaction with midlatitude weather systems. It shows the feature of severe rainstorm dynamic and thermodynamic process. A picture about the process of heavy rain amplification is given.

通过对9608号台风低压及其外围暴雨位涡和等熵面上物理量场的分析，揭示了台风低压北上诱发暴雨过程的位涡场的结构及冷空气对暴雨增幅的作用，给出此次暴雨增幅过程的图像。

It is also shown that one of the most important reasons of the heavy rain amplification is the cold air which spreads to south from midlatitude in the lower troposphere and transports into the typhoon low pressure. The study of PV on isentropic surface shows the active state of the midlatitude cold air. The tropopause folding, which means that high PV spreads downward from the upper troposphere or stratosphere, helps the releasing of potential instability energy and the development of rainstorm.

分析表明：对流层低层中高纬度冷空气扩散南下在台风低压环流区附近的"侵入"作用是此次特大暴雨过程的最重要的原因之一；等熵面位涡的分析进一步说明了中高纬地区冷空气的活动状况；对流层高层或平流层低层高位涡的下传有利于位势不稳定能量的释放，使得暴雨增幅。

There are different types of personal loans available.

有许多不同类型的个人贷款优惠。

The above mentioned teaching model of spiral rising can be used to avoid rigidness in teaching.

上述螺旋式上升的教学模式，可以避免教学形成模式後的僵化现象。