查询词典 affinity constant

To study the function of thyroid hormone receptors in osteoblasts and in bone, the nuclear extracts of TRs from human osteoblast-like cell line MG63 and rat calvaria were achieved, and the two main kinetic parameters of TRs, affinity constant and maximal binding capacity, were determined by radio-ligand binding assay and Scatchard plot.

其中MG63和骨组织中TRs的Ka值与文献报告的人类外周血淋巴细胞以及大鼠脑组织和肝脏中TRs的Ka值相比，差别显著，但与文献中其他成骨细胞系的Ka值相近，提示成骨细胞或骨组织中的TRs与THs的结合能力有可能确实低于淋巴细胞、脑组织或肝脏，从而使得THs在骨组织中的作用机制及效果可能有别于其他组织。

The binding parameters including the average affinity constant,the number of bindingsites and the thermodynamic parameters of this dye bound to HGG were calculatedaccording to Sips method and Van\'t Hoff equation,respectively.And the main actingforces between neutral red and HGG were discussed.

利用Sips方法计算了中性红与HGG反应的平均亲和力常数和结合位点数，根据Van\'t Hoff方程计算了反应的热力学参数，讨论了二者相互作用的主要作用力类型。

In the absence of coorperative effect, the reaction between lectin molecules and their receptors on cell surface can be described as below when equilibrium is reached:In which LT denotes total lectin concentration, LF free lectin concentration, mva-lency of lectin, ni number of receptors with affinity constant Ki to lectin, CT total lectin concentration.

本文提出一个描述细胞与多价凝集素反应的平衡关系式和用紫外分光光度法测定游离凝集素浓度求受体数量及其与凝集素的亲合常数的方便方法。

Keywords porphyrin dimer;DNA;apparent affinity binding constant;photocleavage

双卟啉； DNA；表观键合常数；光切割

The relative cross-reactivity of PA with AFB-1,ZON,T-2,FB were less than 0.01%and with an affinity constant for PA of about 1.54×10~8 liters per mol.It offered a foundation for developing immunoassay method for PA.

单克隆抗体与黄曲霉毒素B1、玉米赤霉烯酮、T-2毒素和伏马毒素B1均无交叉反应率，单抗的亲和常数为1.54x10~8 L/mol，为进一步建立青霉酸的免疫学快速检测方法提供了基础。3。

The Scatchard analysis of data revealed a linear relationship between the amount of specific binding and the ratio of specific binding to free 125 I (D Lys 6)LHRH, indicating a single class of high affinity sites. Equilibrium dissociation constant was 0 34 nM in pituitary membrane preparation and 0 43nM in isolated pituitary cells.

特异性结合随125 I( DLys6 ) LHRH 量的增加而增加，实验结果经Scatchard 分析揭示出特异性结合与结合和游离125I( DLys6)LHRH 之比呈线性关系，预示绍鸭垂体对( DLys6)LHRH 存在同一类高亲和力的结合位点。

The affinity constant of the anti-SQ polyclonal antibody 1405~# was 1.22×10~8L/mol. The cross reactivity with other SAs was less than 0.17%. Three hybridoms cell lines secreting monoclonal antibody against SQ were obtained.

同时，以SQ-BSA为免疫原免疫Balb／c小鼠，经融合实验获得了三株分泌抗磺胺喹噁啉单克隆抗体的杂交瘤细胞株SQl_(12)A_(10)，SQ_(26)B_7，SQ_(28)H_7。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

This paper analyses several kinds of charge algorism such as constant current, constant voltage and intelligent charge theoretically.

从理论上分析了恒流、恒压和智能等充电算法。

Using analytic continuation in the coupling constant method within the framework of the self-consistent relativistic mean filed theory, we study the energies and the widths of the resonant states in ~(16)O nuclei, and the influences of the coupling constant interval and the Pade approximant order are analyzed.

考察了~(16)O的中子共振态能级，并且详细研究了耦合常数右端点对应能量的变化和Padé多项式的阶数对能量和宽度的影响。结果表明：适当选取耦合常数取值区间可以得到既收敛又稳定的结果。

We have limited budget let me know if you can do this competitively.

我们有限的预算让我知道如果你能做到这一点竞争力。

We don't know how other critters breathe--lizards, for example.

我们还不知道诸如蜥蜴之类的动物如何呼吸。