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Lysis solutions from imported and domestic genomic DNA purification kits,and 2% Trition X-100 and 5%guanidinium isothiocyanate were added into the oocysts,followed by different combining forms of freeze-thawing,proteinase K and sonication.Real-time PCR was used to determine the copies of Cryptosporidium oocyst wall protein gene.HCT-8 cells were infected by C.andersoni oocysts.Real-time PCR was used to determine the enhancement effect of fetal calf serum,glucose,ascorbic acid,folic acid,calcium pantothenate and insulin while added into RPMI-1640 medium.
在隐孢子虫卵囊悬液中分别加入进口试剂盒和国产试剂盒的裂解液以及2%Triton X-100和5%异硫氰酸胍,辅以不同组合方式使用的冻融法、蛋白酶K法和声裂法裂解卵囊,通过Real-time PCR法测定裂解产物中隐孢子虫卵囊囊壁蛋白(Cryptosporidium oocyst wall protein,COWP)基因拷贝数。
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In group A, glutamine was added enterally, and in the group B, glutamine was added parenterally.
测定3组的转铁蛋白、前白蛋白、氮平衡、免疫球蛋白、CD4/CD8、感染发生率等指标。
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Methods Synaptosomes was made from hippocampus and incubated with artificial cerebrospinal fluid. 30μmol/L propofol, 50μmol/L picrotoxin or both was added into aCSF. Intralipid was administered as solvent control and any drugs was applied in control. To observe Ca~(2+)-dependent release of glutamate and GABA, dihydrokainic acid and nipectic acid were added into aCSF.
制备大鼠海马突触体,随机分为5组:PTX组加入印防己毒素使其终浓度为50μmol/L,Pro30+PTX组加入终浓度为30μmol/L的丙泊酚和50μmol/L的PTX,Pro30组加入终浓度为30μmol/L的丙泊酚,溶剂对照组加入脂肪乳,空白对照组不加入上述药物。
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The first single Mademoiselle Juliette got on the top 13 of French downloading board with its passionate electronic and rock mixture. Alizée also added some cool and stylish singing skills in it. She invited French well-known producer and music composer Bertrand Burgalat (Depeche Mode、Pizzicato Five、Mick Harvey) to produce this album. The second song Fifty Sixy is with smooth and great melody also add some cheerful dance sounds that totally shows extremely young passion. The song with same title as album Psychédélices is set in a fantastic and air-free atmosphere. Beautiful violin sounds go through the whole song and gradually lead the song the softest and most fascinating feeling. Decollage is a song that is added with hip-hop street style. In this song, Alizée shows her great talent in rapping that makes people feel refreshing. With fast beats, Lilly Town is just in people's taste of dance song. The last song of this album L'effet spreads a slight tender of jazz. This time Alizée use various styles that add more charm with her mature appearance.
第一波单曲" Mademoiselle Juliette",登上法国下载榜Top13,交融强烈电子与摇滚的撞击,艾莉婕加入更多酷酷个性演唱调调,请到法国籍炙手可热的制作/演奏才子 Bertrand Burgalat(Depeche Mode、Pizzicato Five、Mick Harvey)操盘;第二支预备曲"Fifty Sixy",更为流畅动听旋律贯穿,同样加料欢乐舞曲声韵,展现年轻的无比热力;同名单曲"Psychedelices"漂浮在一股迷离无压的气氛之中,弦乐优雅润饰其中,徐缓勾引出最动人的抒情乐章;添进街头嘻哈风格的"Decollage",艾莉婕更大秀饶舌才情,让人耳朵为之一开;节奏加速度的" Lilly Town",是支十分讨喜的正点舞曲;谢幕小品"L'effet"则洒下一抹淡淡爵士柔情,艾莉婕此次大胆尝鲜更为多样风格,为她成熟风貌添加更多迷人魅力。
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Tergitol or PEG (0.5 g) was added to 10 mL of a 20% solution of alkyl halide in phenylacetonitrile, and then this solution was added to 10 mL of 60% aqueous potassium hydroxide.
Tergitol或聚乙二醇( 0.5 g )项增加了10毫升的 20 %的溶液中苯乙腈烷基卤化物,和那么这个解决办法是增加了10毫升的60 %水氢氧化钾。
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I've done the colar, added more wrinkles, and added a pouch on the arms.
我已经做了科拉尔,增加更多的皱纹,并增加了一个邮袋的武器。
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As a result of the JET review the program was restructured, requiring an additional $2.2 billion be added to the EMD budget and 12 months be added to the schedule to ensure the achievement of a producible, affordable design prior to entering production.
由于射流的审查程序进行了调整,需要额外的220亿美元增加至emd预算和12个被列入附表确保实现了producible负担的设计,然后才进入生产。
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One is a control with no alkyl malonic acid added and the others had 5% by weight of propyl malonic acid or isopropyl malonic acid added.
香料烟化学成分一个引人注目的特征是含有较多的低级脂肪酸,其中异戊酸和β—甲基戊酸的含量是烤烟的几百倍以上。
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The results showed that organic blend acids preparation improved average daily gain and decreased feed gain ratio,and the effect in the group added 5 g/kg was better;pH in proventriculus decreased along with the increase of organic blend acids preparation added in the basal diet and was relatively stable...
结果表明,饲料中添加有机复合酸化剂能够提高艾维因肉仔鸡日增重、降低料重比,而且以5 g/kg添加组效果最好;其腺胃内pH随有机复合酸化剂浓度的增加而降低,但到第5 d各组达到了相对稳定;饲料中蛋白质的消化率以15 g/kg添加组效果最好,干物质、总能和植酸磷的消化率以5 g/kg添加组最高。
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When it comes to bread, Davie, and co-author, nutritionist Dr Joanna Mcmillan Price, have given five star ratings to pumpernickel, sourdough breads made with whole grain or rye and the heavier wholegrain breads made from wholewheat flour and added grain. Wholemeal, however, doesn't rate so highly - neither does multigrain bread, which Davie and McMillan Price say is made with white flour with a few grains added.
当谈到面包时,Davie和另一名合著者,Joanna Mcmillan Price博士,已予黑面包,全谷物或黑麦发酵面包,以及全麦粉加谷物制成的较重的全麦面包五星级别,全麦面粉却没有得到如此高的级别--杂粮面包也没有,Davie和Mcmillan Price说,杂粮面包是用白面加谷物做成的。
- 相关中文对照歌词
- I Guess I Planted
- A Handful Of Dust
- Handful Of Dust
- A Handful Of Dust
- We Added It Up
- Forsaken
- No Charge
- Are You Online?
- Circuit Breaker
- Opening The West
- 推荐网络例句
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Must have Coordination and negotiation skill, flexible ideas, and be punctilious person.
必须具备较好的协调与谈判能力,头脑灵活,工作认真仔细
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Result: 18 patients are all with simple eye.The pigment mole lies in nasal and temple sides of the ball conjunctiva,in which 2 lie in lacrimal caruncle and semilunar plica.
结果:18例患者均为单眼,色素痣大多数位于内外侧睑裂部球结膜面,2例位于泪阜及半月皱襞,经激光治疗后,10例色素痣消失,有5例行再次激光后消失,2例行第三次激光后消失,1例第四次激光后消失。
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Buy the home shilly-shally to see when group the person is in when speaking highly of this net inn, absolutely meeting gives up of the apprehension in the heart.
当一个犹豫不决的买家看到一个群的人都在夸赞该网店时,绝对会打消心中的顾虑的。