查询词典 added

According to the Interim Regulation of the People's Republic of China on Value Added Tax (Order No.538 of the State Council, hereinafter referred to as the "Regulation"), the Detailed Rules for the Implementation of the Interim Regulation of the People's Republic of China on Value Added Tax (Order No.50 of the Ministry of Finance and the State Administration of Taxation) and the relevant spirit of the State Council, to do a good job in the consistency of the relevant value added tax policies and strengthen the administration of collection of value added tax, we hereby clarify the relevant issues concerning applying low value added tax rates and policies on collecting value added tax by the simple approach to some goods as follows

各省、自治区、直辖市、计划单列市财政厅、国家税务局，新疆生产建设兵团财务局：根据《中华人民共和国增值税暂行条例》（国务院令538号，以下简称条例）和《中华人民共和国增值税暂行条例实施细则》（财政部国家税务总局令50号）的规定和国务院的有关精神，为做好相关增值税政策规定的衔接，加强征收管理，现将部分货物适用增值税税率和实行增值税简易征收办法的有关事项明确如下

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

In sparkle discharge ,following conclusions could be reached:(1) overall spectral intensity in nitrogen is lower than in oxygen . When water is added, integrality light intensity is lower compared with the pure gas condition.(2) No matter it is nitrogen or oxygen, when water is added, syllabify three spectral lines of atomic H: Hα(656.3nm), Hβ(486.1nm), Hγ(434.0nm) could be seen. What is more, when water is added into oxygen, the spectral line of H atom becomes stronger.(3) When discharge in pure nitrogen, the light intensity falls with the increasing gas flow rate. While if water is added, the light intensity will rises with the increasing gas flow rate.(4) When discharging in pure oxygen, the light intensity rises with increasing gas flow rate. While if water is added, with increasing gas flow rate the light intensity initially will change as this:first rise, then saturate at about 140l/h, finally falls.

在火花放电中：(1)氮气的整体光谱强度要弱于氧气的整体光谱强度，加水时的整体光谱强度比纯气体中放电小很多；(2)无论所通为氮气或氧气，加水时可以清晰的看到Hα(656.3nm)，Hβ(486.1nm)，Hγ(434.0nm)三条氢原子的谱线，而且在氧气加水的情况下产生的H原子的谱线要强些；(3)纯氮气中放电时随着气流量的增大放电光谱的强度随之降低，氮气加水放电时随着气流量的增大放电光谱的强度随之升高；(4)氧气放电时，随着气流量的增大，放电光谱的强度随之升高，氧气加水中放电时随着气流量的增大，放电光谱的强度开始随之升高，在140l/h时的值最高，接着就开始下降。

The influence of battery formula and technology parameter on the safety performance of mercury-free alkaline Zn/MnO2 button cell was explored. Short circuit test and overdischarge test were used to study the safety performance of battery produced with varied formula and technology parameter. The factors affecting the safety performance of battery were studied. The optimal technology parameters are confirmed as follows: capacity ratio of anode to cathode is 0.91, KOH electrolyte content is 43%, content of Zn22- dissolved in KOH electrolyte is 7%, content of ZnO added in zinc gel is 0.8%1.0%, content of electrolyte added in zinc gel is 40%42%, content of binder added in zinc gel is 1% and the mass ratio of polyacrylate sodium to polyacrylic acid is 64, content of inhibitor added in zinc gel is 0.01%.

摘 要：探讨电池配方和工艺参数对无汞扣式碱锰电池安全性能的影响，采用短路测试和过放电测试的方法研究各种工艺配方下电池的安全性能，通过实验研究了影响电池安全性能的各种因素，并确定最佳的工艺参数分别如下：负极与正极容量配比为0.91，KOH电解液浓度为43%，电解液中氧化锌的含量为7%，锌膏中的氧化锌用量为0.8%~1.0%，锌膏中电解液用量为锌粉量的40%~42%，锌膏中增稠剂用量为锌粉量的1%且其中聚丙烯酸钠与聚丙烯酸的质量比为64，锌膏中缓蚀剂用量为锌粉量的0.01%。

Companies in the mobile value-added services, fixed-line value-added services, network communications areas of value-added services with a strong product development, marketing and customer service capabilities, and give full play to the company management and staff at all levels, accumulated over the years in the field of telecommunications value-added services Experience

公司在移动增值服务领域，固网增值服务领域，网络通信增值服务领域都具备强大的产品开发，营销和客户服务的能力，并充分发挥公司各级管理人员和员工多年积累的在通讯增值服务领域的经验

In 300mg/kg JSS treatment, the yolk color was 6 degree too. While added 500mg/kg JMH into the basal diet, the egg yolk color was up to 9 degree. Supplement of oxytetracycline + JMH or chlortetracy dine + JMH in the basal diet, the egg yolk color was up to 8 and 6 degree respectively and decreased one and three color unit respectively than that of JMH treatment. Added oxytetracyclimne + JSS or chlortetracycline + JSS, the egg yolk color was up to 8 and 6degree respectively. When added arsanilic acid + JSS, the egg yolk color was up to 7 degree and improved one color unit than that of JSS treatment. When added arsanilic acid +JMH to basal diet, the egg yolk color was up to 8 degree and decreased one color unit than that of JMH treatment.

饲料中添加300mg/kg的金闪闪，蛋黄颜色也是6级，没有产生增色效果，添加500mg/kg的加美黄，可使蛋黄颜色达到9级，提高3个比色单位，土霉素与加美黄配合使用，蛋黄颜色为8级，比加美黄组降低1级，金霉素与加美黄配合使用蛋黄颜色为6级，比加美黄组降低3级，土霉素与金闪闪配合使用可使蛋黄颜色升到8级，比金闪闪组提高2级，金霉素与金闪闪配合使用蛋黄颜色仍为6级，阿散酸与金闪闪配合使用，蛋黄颜色为7级，比金闪闪组提高1级，阿散酸与加美黄配合使用蛋黄颜色为8级，比加美黄组降低1级。

Color reaction was performed in organic solution-salt solution biphasic system. The organic solution was 1,2-Dichloroethane-Isoamyl alcohol mixture (V/V=96:4) added Bromophenol blue (per 100 mL added 0.05 g). The salt solution was 55%K2HPO4 aqueous solution added Na3CO3 (per 100 mL added 14 g), under the temperature of color reaction between 12℃ to 25℃.

显色反应在有机溶液－盐溶液两相体系中进行，有机溶液为每100 mL 1，2-二氯乙烷－异戊醇混合溶液（体积比96：4）加入0.05 g溴酚蓝，12~25℃显色反应条件下，盐溶液为每100 mL 55%K2 HPO4水溶液加入14 g Na2CO3。

With the observation of the outward appearances, it can be known roughly that if the sample was added with adulterations. Since the beeswax dissolves in the benzene but the adulterations, such as gypsum, starch and so on do not, these can be determined. By the verification of acid value and alcohol dissolve ability, the acid or non acid added can be determined. By the change of saponification value, the ester or non ester added can be determined. By the carbonification degree in the concentrated sulphuric acid, the mineral wax, such as paraffin wax added can be determined.

通过样品外观的观察初步定性是否掺有杂质；利用纯蜂蜡在苯中溶解，而掺入的石膏、面粉和淀粉等杂质在苯中不溶而定量鉴定这类杂质；由酸值变化和乙醇溶解物的量，测定掺入的硬脂酸等酸性物质或其它的非酸性物质；由皂化值变化，定量测定掺入的动物脂等酯类物质或其它的非酯类物质；根据蜂蜡中的烃类在浓硫酸中可被炭化，加入的石蜡等矿物蜡不被炭化来测定此类掺杂物。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

A preparation method of the visible-light photocatalyst Bi2WO6 nano-powder is characterized by comprising the steps as follows:(1) the selection of raw materials;(2) bismuth nitrate Bi(NO33 question mark 5H2O is added into a citric acid solution to obtain the citric acid solution containing white sediment;(3) EDTA is added into ammonia water which is then added dropwise with the citric acid solution containing the Bi(NO3)3 question mark 5H2O so to obtain a solution of bismuth;(4) the solution of bismuth is added with (NH4)6W7O24 question mark 6H2O solution to form a precursor solution which is then stirred, dried, coked, cooled and ground to obtain decarburized powder;(5) the decarburized powder is roasted for 2h to 4h under 450 DEG C to 550 DEG C to obtain the visible-light photocatalyst Bi2WO6 nano-powder.

可见光催化剂Bi 2 WO 6 纳米粉体的制备方法，其特征在于它包括如下步骤：1原料的选取；2将Bi(NO 3 ) 3 5H 2 O加到柠檬酸溶液中，得到含有白色沉淀的柠檬酸溶液；3将乙二胺四乙酸加到氨水中，并缓慢滴加入含有Bi(NO 3 ) 3 5H 2 O的柠檬酸溶液中，得铋的溶液；4将(NH 4 ) 6 W 7 O 24 6H 2 O溶液加入上述铋的溶液中，组成前驱液；搅拌，干燥，焦化，冷却后，研磨，得去碳的粉体；5将去碳的粉体在450～550℃下焙烧2～4h后，得到可见光催化剂Bi 2 WO 6 纳米粉体。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。