查询词典 Trypan

MAIN OUTCOME MEASURES: The amount of trypan blue absorbed by the CAM. RESULTS: The correlation coefficient between CAM-TB score and Draize eye irritation was 0.925 9. Glycerol had no eye irritation potential, while acet, alcohol, methanol and DMSO had.

结果：鸡胚尿囊绒膜-锥虫蓝吸收法与体内兔眼刺激实验评分结果相关性很好（R2=0.925 9）。5种常见有机溶剂未稀释时，甘油无眼刺激性，丙酮、乙醇、二甲基亚砜、甲醇均有较高刺激性。

The result reveals that, The cow colostrums contains a large number of the somatic cells: SCC is 200,000 /mL, the activity of the milk somatic cell is 60% byusing Trypan blue exclusion. This somatic cell can be used to carry on the cell ulav analysis and RNA extraction, the integrality and purity quotient of the extracted RNA can be used for prefabricating cDNA.

结果：牛初乳中含有大量的体细胞（SCC为20万／mL，台盼蓝拒染测得活力为60%），可以利用提取的体细胞进行细胞分析以及RNA的提取，所提取RNA的完整性和纯度可以用来合成cDNA，看家基因GAPDH扩增条带清晰。

Trypan-blue and alizarin red vital staining was capable of displaying the cellula appearance and distinguishing the normal and abnormal cells.

光镜下可清楚显示晶状体上皮细胞的形态并能区分正常与死亡的细胞。

Expressions of PLK1 mRNA and protein were assayed by RT-PCR and Western-blot; cell proliferation was evaluated by direct cell counting after trypan blue staining.

PLK1基因沉默能有效抑制K562/A02细胞生长，使细胞周期停滞在G2/M期，同时使得细胞内ADM积累量提高，对ADM敏感性增强。

METHODS: SGC7901 cells were transfected with siRNAs targeted against the human PLK1 by chemosynthesis. The PLK1 mRNA levels of cells transfected with siRNAs were monitored by real time PCR and the protein levels were detected by immunocytochemistry. Cell proliferation was evaluated by direct cell counting with trypan blue staining.

化学合成针对PLK1的小干扰RNA，转染至胃癌细胞株SGC7901中，利用实时定量PCR、免疫细胞化学、台盼蓝活性细胞计数和流式细胞术对PLK1的基因表达、细胞增殖、细胞周期和凋亡的检测。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

The proliferation of cells were detected by trypan blue exclusion, methyl thiazolyl tetrazolium assay, Hoechst33342 fluorescence staining and flow cytometry method.

利用台盼兰排斥试验、噻唑蓝比色法、Hoechst33342 荧光染色、流式细胞术测细胞周期法等实验技术分别检测了不同浓度AA 和DHA 对大鼠前体脂肪细胞活力、细胞周期及凋亡的影响。

Detdcting method of cellular vital force: the teypan blue execlusion test wasselected and after stanging with 0.4% trypan blue counted vital cell could be above 95% in three minutes.

4人外周单一核细胞的分离技术慢性荨麻疹患者68例和正常人24例体外抽取患者新鲜静脉血5ml，采取Ficoll等密度沉降分离法，得到淋巴细胞和单核细胞，以RPMI1640培养液定容，制成细胞悬液，细胞浓度为1×10~6／ml。

This matter overthrew all basises I learned from Sunday school .

这件事把我从周日学校学到的所有道理都颠覆了。

It's one of the cruel ironies of aging: if you want to remember your favorite restaurants, the less you should eat at them.

这是一个老化的残酷讽刺：如果你想记住你最喜欢的餐馆，你应该更少吃到它们。