查询词典 Trypan

He CD4〓 T-cell viability determined by the trypan blue test was over 90%. PBMCs from the recipients, after inactivated, were added in 96-well culture plates with donor CD4〓 T-cell cocultured at 37℃ for 24 hours, TJU103 and CTLA4-Ig were given for additional 48 hours coculture. The CD4〓 T-cell was collected and washed as anergic CD4〓 T-cell.

JU103联合CTLA4-Ig应用对供者T细胞增殖活性的影响根据TJU103有剂量限制性特点，选择TJU103最大抑制效应浓度50μg/ml，与不同浓度CTLA4-Ig（1μg/ml、10μg/ml、50μg/ml、100μg/ml）联合，对供者T细胞的增殖抑制率分别为（59.5％、90.5％、93.4％、95.8％），TJU103（50μg/ml）与低浓度CTLA4-Ig（10μg/ml）联合，增殖抑制率在90％以上，已接近最大增殖抑制率，为二者联合应用的最佳组合，与单独应用组比较，有显著的统计学差异（p＜0.05）。

Methods 0, 2.5, 5, 10, 15 and 20 μmol/L arsenious acid-treated human hepatocellular carcinoma HepG2 cells were cultured under hypoxia for 8 hours. The cell proliferation and cell viability was assayed by WST-8 and the trypan blue dye exclusion method. The expressions of HIF-1α in human hepatocellular carcinoma HepG2 cells were checked by RT-PCR and Western Blot.

以0、0.25、0.5、1、2和4μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧环境中培养8h；1μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧培养0~10h，WST-8法和台盼蓝染色法分析细胞增殖和活力，采用RT-PCR和Western Blot方法检测肝癌细胞中HIF-1α的表达。

The inhibition of PC3 ceils proliferation by AD-HOSM was determined by trypan blue exclusion ceil count assay.

结论重组腺病毒介导HOSM表达能有效抑制PC3在体外的增殖，提示重组腺病毒介导的HOSM基因治疗可能成为胰腺癌治疗的候选方案。

Bone marrow was drawn from rabbit by density gradient centrifugate in 70% Percoll fluid, the rate of cell survival was evaluated with trypan blue staining;The cells have been induced,differentiated and transfered of culture in vitro for five weeks; MSCs was seed onto BMG and continued to be cultured six weeks in vitro,then the compound was carried out to observe collagen by Masson staining.

抽取兔骨髓，用70% Percoll液，经密度梯度离心法分离MSCs，台盼蓝染色观察细胞存活率；诱导分化，传代，体外培养5周；复合BMG，继续体外培养6周，Masson染色观察胶原合成情况。

Trypan blue exclusion assay and cell growth curve were used to observe proliferation. Fluctuation of CD11b, reactive oxygen species and NF-кB were detected using flow cytometry. Cell apoptosis was observed using DNA laddering assay.

选择NB4细胞，加入ATRA和不同剂量的As2O3，观察细胞生长曲线，流式细胞仪检测CD11b、ROS、NF-кB的变化，DNA片段梯度观察细胞凋亡。

Methods The logarithmically growing CEM cells cultured in vitro ( cell density 2 × 105/mL) were exposed to 0.1, 0.5, 1, 5, and 10 μM Dex, then were collected 24, 48 and 72 hrs later. Untreated CEM cells were used as Controls. The cell viability was determined by trypan blue dye exclusion.

方法将在体外培养的对数生长期的CEM细胞浓度调至2×105个/mL，接种于24孔培养板中，用终浓度分别为0.1，0.5，1，5，10 μM地塞米松处理，以不加任何药物的CEM细胞作为对照组，培养后24，48，72 h取样。

In Vitro study on the impact of overexpression of RbAp46 in U937cell lineObjective To study the impact of overexpression of RbAp46 in monoblastic cell line U937. Methods Viability of transfected cells was assayed by trypan blue exclusion.Cell number was counted daily to determine the growth rate.

将处在对数生长期U937／RbAp46和U937／CMV各以2×10~5／ml浓度接种，加入TPA50ng／ml，同时并以培养基作为空白对照，培养72小时后收集细胞，流式细胞术测定细胞表面分化抗原CD11b的表达，RT-PCR分析p21mRNA的表达，流式细胞术检测细胞周期分布情况。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

Supravital stains ; Sperm ; Eosin ; Trypan blue ; Nigrosin ; Mouse

小鼠精子；活体染色；伊红；锥兰；苯胺黑

Who? I never heard of him, Paul said, before asking teammate James Posey if he had heard of him.

赛后，科比说，他一直都是一名非常出色的射手，今天他打得很棒。

When I joined the company, I rotated around the different sections.

我加入这个公司时，轮换过几个不同的部门。