查询词典 Trypan

A new method to identify _ amylase activity and its producing bacteria : the blue complex was formed by unspecific adsorption , after mixing starch and trypan _ blue . the adsorption weakened when the starch was hydrolyzed to small molecular by _ amylase , and the trypan _ blue was released inside the hydrolyed zone . the starch around the zone which was not hydrolyzed adsorbed free trypan - blue so that the colour of medium became bluer than that of place in hydrolyzed zone

快速鉴定并筛选-淀粉酶及其产生菌的新方法：锥虫蓝染料和淀粉由于静电非特异性吸附结合后使淀粉呈稳定的蓝色，当淀粉被淀粉酶水解后因分子变小吸附力减弱，而让锥虫蓝游离出来，游离的锥虫蓝被周围未水解的淀粉吸附而使颜色加深，淀粉水解区则形成无色、透明的水解圈。

The growth curve of cellular clone was determined by trypan blue exclusion test.

RT-PCR和Western Blotting检测PNP-TK基因在HepG2细胞中的表达。

Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA.

采用人结肠癌细胞株DLD1，利用人工合成的Bcl-XL靶向小分子干扰RNA转染DLD1细胞，通过Western blot法检测转染细胞Bcl-XL蛋白的表达，流式细胞仪检测处理后细胞的凋亡情况，锥虫蓝染色法计数存活细胞。

Quiescent RASMCs were incubated respectively in 10% FBS DMEM as control group, 10% FBS DMEM supplemented with 0.1% dimethyl sulfide as vehicle group and 10% FBS DMEM supplemented with panaxynol(at concentration of 1,3,9×10-6 mol·L-1) as panaxynol groups. 1. Cell counting and double time. Cell viability was examined with trypan blue exclusion test and the cell number was counted at 0, 24, 48, 72h after the incubation using a hemocytometer.

实验中细胞分成如下几个处理组：①正常对照组：含10％FBS的培养液；②溶剂对照组：0.1％DMSO；③药物处理组：panaxynol 1、3、9×10-6 mol·L-1组。1、细胞计数及倍增时间测定：于接种并同步化后处理0、24、48、72h，用血细胞计数板计算活细胞数量，绘制生长曲线并计算细胞倍增时间。

In our study, we found menadione has anticancer potential on human breast adenocarcinoma MDA MB 231 by weather cell cycle arrest, anti proliferation, or apoptosis. Low concentration of menadione (5.8μM) significant inhibited growth curve on breast cancer cells (MDA MB 231) but not breast cells (MCF 10A) by performing MTT and trypan blue cell counting. RT-PCR and western blotting showed menadione increase p21 gene and protein expressions but decrease cyclin E and CDK2 protein expression resulting in cell cycle G0/G1 arrest by flow cytometry.

MTT及生长曲线的实验，发现menadione在低浓度且长时间作用情况下，对在於人类乳腺上皮细胞癌(MDA-MB 231)是有明显的抑制作用，而在正常乳腺细胞(MCF-10A)则没有影响，在分子转录上及蛋白质表现方面也发现低浓度menadione处理24时能使MDA-MB 231诱发p21基因及蛋白表现量增加及cyclin E、CDK2抑制，再经由流式细胞仪分析也发现低浓度 menadione 处理24小时能使MDA-MB 231细胞周期GO/G1比例上升，造成细胞周期停滞，进而抑制细胞生长。

The dye Trypan Blue has a remarkable biphasic effect on pinocytosis by yolk sal.

染料锥虫蓝对卵黄囊的胞饮作用具有明显的双向作用。

Establishment of a bovine mammary tissue culture system Bovine mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and ultrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation.

1奶牛乳腺组织体外培养方法的建立采用组织灌流和组织块贴壁法体外培养奶牛乳腺组织，通过LDH活力测定、台盼兰染色、琼脂糖凝胶电泳、透射电镜技术比较两种培养方法对奶牛乳腺组织活性及组织超微结构的影响，建立奶牛乳腺组织的培养体系。

To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量；薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用，确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化，流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

The area at risk and infarct area were determined using Trypan blue and triphenyl tetrazolium choride staining respectively.

再灌末取心肌组织，以四氮唑染色法测定心肌梗死面积(IS/AAR×100%)。

Experimentally, multi-pores are observed on leukocyte lysed with 3D electrodes and just single pore with planar electrodes. This is believed to be due to more uniform electric field of 3D electrodes than that of planar electrodes. Intracellular elements are extracted through the membrane as observed by increased intensity of Trypan blue when diluted by saline solution. We also demonstrate the lysing percentages at same flow velocity to explain the performance of 3D electrode is superior to that of planar electrode.

在实验方面，可发现细胞在3D 电极的形况下，是有多个破孔出现，有别於在平面电极的单个破洞，也发现当细胞出现破孔时，经由Trypan blue的辅助观察以及在食盐水稀释情况下，细胞内液是往外流出的，另外由裂解比率的结果，3D电极也是优於平面电极。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。