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Results The activity of FⅫ were 52.5%, 78.6% and 89% for the proband, his father and mother, respectively. Direct sequencing suggested that the proband was 46 T/T genotype in exon1, while both his parents were 46 C/T genotype in the same alle.

结果 先证者及其父、母FⅫ：C分别是52.5%、78.6%、89%，直接测序结果提示先证者F12基因的第1外显子46位碱基即启动子上游-4位为T/T基因型，其父、母在该位点均为C/T基因型。

This subset is hyporesponsive and anergic to antigen stimulation. Recently, it has been demonstrated to participate in the immune deficiency in human immunodeficiency virus infection and has close relationship with the development of acquired immunodeficiency syndrome. Meanwhile, the CD4(superscript +)D25(superscript +)Treg is also infected when the human body is invaded by HIV. The CD4(superscript +)CD25(superscript +)Treg will finally be killed by the mechanism of antibody-dependent cell-mediated cytotoxicity. Because of the decrease of the CD4(superscript +)CD25(superscript +)Treg, it can't function as a immune suppressor as a result of the excess of activation of HIV and gradually exhausting of the T cell, which shows that the CD4(superscript +)CD25(superscript +)Treg plays a inhibitory role in the different stages of the development of the HIV as well as different effects in the pathogenesis of AIDS.

它能够抑制自身免疫病的发生和发展，参与肿瘤免疫的调节，同时在感染和移植免疫中也发挥着极其重要的作用。T细胞的这一亚群具有免疫调节和免疫抑制的特性，新近发现它亦与爱滋病的发生、发展关系密切HIV进入人体后，CD4D25调节性T细胞抑制了机体的免疫效应但它也同时被感染，最终由于细胞毒的作用而死亡由于调节性T细胞数量的减少不能有效的发挥其抑制作用，HIV持续的过度活化使得T细胞逐渐耗竭说明在HIV发生、发展的不同阶段Treg细胞可能都发挥了免疫抑制作用，但是却对HIV感染与爱滋病发病的进程产生了不同的效应。

After inactivated by mito C, the anergic CD4〓T-cell was added in medium of new isolated the same donor CD4〓 T-cell and the recipient CD4〓 T-cell respectively, the same experiments with the anergic CD〓T-tell and supernatants.

TJU103联合CTLA4-Ig应用，对供着T细胞特异性增殖和细胞毒活性的影响 TJU103联合CTLA4-Ig应用能够抑制供者T细胞增殖，A492nm值为0.032±0.004，且具有抗原特异性。

He CD4〓 T-cell viability determined by the trypan blue test was over 90%. PBMCs from the recipients, after inactivated, were added in 96-well culture plates with donor CD4〓 T-cell cocultured at 37℃ for 24 hours, TJU103 and CTLA4-Ig were given for additional 48 hours coculture. The CD4〓 T-cell was collected and washed as anergic CD4〓 T-cell.

JU103联合CTLA4-Ig应用对供者T细胞增殖活性的影响根据TJU103有剂量限制性特点，选择TJU103最大抑制效应浓度50μg/ml，与不同浓度CTLA4-Ig（1μg/ml、10μg/ml、50μg/ml、100μg/ml）联合，对供者T细胞的增殖抑制率分别为（59.5％、90.5％、93.4％、95.8％），TJU103（50μg/ml）与低浓度CTLA4-Ig（10μg/ml）联合，增殖抑制率在90％以上，已接近最大增殖抑制率，为二者联合应用的最佳组合，与单独应用组比较，有显著的统计学差异（p＜0.05）。

Since prince was on apollonia since OJ had isotoners don\'t act like I never told ya baby you\'re making it (harder, better, faster, stronger) Now that don\'t kill me can only make me stronger I need you to hurry up now cause I can\'t wait much longer I know I got to be right now cause I cant get much wronger man i\'ve been waitin all night now that\'s how long i\'ve been on ya I need you right now you know how long i\'ve been on ya?

由于王子是apollonia 由于法院组织了isotoners 唐\用' t好像我从来没有告诉雅宝宝你\ '重新使（更难，更好，更快，更强）现在唐\用' t杀了我只能使我更有力我需要你，赶快现事业，我可以\用' t等待更长我知道我是正确的，现在事业i cannot获得多少wronger 男子i \ ' ve的被waitin整夜现在这\ ' s多久i \ ' ve的一直是亚我需要你的权利，现在你知道有多长i \ ' ve的一直是雅？

Its functions can be divided into three subsets: T helper cells, inhibition of cytotoxic T cells and T cells.

按其功能可分为三个亚群：辅助性T细胞、抑制性T细胞和细胞毒性T细胞。

Results the mean time of removed t-tube in patients with biliary leak were (28.7±7.3) days,the mean serum albumin level were (33.4±2.5)g/l,the mean hemoglobin level (116.5±17.3)g/l;while the mean time of removed t-tube in patients without biliary leak were (24.3±6.5)days,the mean serum albumin level were (37.9±1.7)g/l,the mean hemoglobin level (143.4±12.7)g/l.conclusion dystrophia is the reason of most biliary leak after removed t-tube.

结果 拔t管后胆漏病人的平均拔管时间为(28.7±7.3)天，血浆白蛋白(33.4±2.5)g/l，血红蛋白(116.5±17.3)g/l；无胆漏病人t管拔除的平均天数是(24.3±6.5)天，血浆白蛋白(37.9±1.7)g/l，血红蛋白(143.4±12.7)g/l。结论 t型引流管拔除后胆漏的主要原因是病人的营养状态差。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞，建立小鼠T淋巴细胞活化、增殖的模型，以不同浓度的CPT作用于该模型，流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

For example, big boot tele-ers will want to try the T-1, the Gara and the Crispi CX-1 or -2, mid range boot buyers would want to try on the T-2, Veloce and Cx-4, lighter boots to try would be the Scarpa T-3's and Garmont's Libero.

比如，喜欢重型鞋的tele滑雪者应该试试T-1、Gara和Crispi CX-1或CX-2；偏好中型鞋的应该试试T-2、Veloce和CX-4；喜欢轻型鞋的应该试试Scarpa T-3和Garmont Libero。

The dynamic changes of the number of T or B lymphocytes and the content of immunoglobulin G, IgM, IgA in peripheral blood, the number of CD〓, CD〓 lymphocyte subpopulation, T〓 cells and IgG, IgM, IgA antibody-producing cells, the ratios of CD〓 and CD〓 T cells in immune organs (thymus, bursa of Fabricius and spleen) and immune tissues (cecal tonsils and Harderian glands), the content of IgA, IgM, IgG in local fluid (tear, tracheal fluid, intestinal fluid and bile) of one-day chicks infected with chicken infectious anemia virus and CIAV-infected chicks immunized with Newcastle disease vaccine were determined respectively by immunohistochemistry, immunogold-silves and immunoenzyme staining in order to reveal mechanism of immunopathogenesis of chicks infected with CIAV and influence of immune responses of CIAV-infected chicks to ND vaccine.

中文题名雏鸡感染鸡传染性贫血病毒后免疫变化和对新城疫疫苗的应答及其免疫机理副题名外文题名 Immune changes of chicks infected with chicken infectious anemia virus and immune response and mechanism to Newcastle disease vaccine immunity 论文作者郑世民导师刘忠贵教授学科专业基础兽医学研究领域\研究方向学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数105页关键词鸡传染性贫血病雏鸡新城疫疫苗馆藏号BSLW /2003 /S858 /9 本项研究采用免疫组织化学、免疫胶体金及免疫酶方法，首次对CIAV感染1日龄雏鸡和CIAV感染雏鸡ND疫苗免疫及vNDV攻击后，其外周血液的T细胞、B细胞数量和IgG、IgM、IgA含量；中枢免疫器官胸腺和法氏囊，外周免疫器官脾脏及呼吸道和消化道局部粘膜免疫组织哈德尔腺和盲肠扁桃体的ANAE〓、CD4〓、CD〓T细胞、IgG、IgM、IgA抗体生成细胞数量和CD〓与CD〓T细胞比值以及泪液、气管液、肠液、胆汁的IgA、IgM、IgG含量的动态变化规律和CIAV感染对ND疫苗的免疫保护率等进行了全面系统地研究。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。