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Methods 58 cases patients needing endocardiac orthoptic surgery were enrolled in this study, they are divided into control group and SFI group randomly. Injecting of SFI 1ml/kg by intravenous before CPB was performed in group SFI, tidal volume, Pmax , Pmean and calculating Crs and RI during 10 min , 30 min , 60 min after the trachea intubating, in the beginning of CPB and during 10min, 60min, 120min after CPB were recorded.

选择临床心内直视手术病人58例，随机分为对照组和SFI组，SFI组于CPB前予SFI 1ml/kg静注，记录气管插管后10、30、60 min，CPB手术开始时，CPB停止后10、60、120min时的潮气量、气道峰值压、平均气道压并肺顺应性和呼吸指数。

Objective The anticogant to the endotheliotoxin receptor is a promising drug to treat with the pulmonary arterial hypertension therotically.But in our country there was few systemical clinical trial about the drug used in the disease.We study the curative effect and the side effect of an anticogant to the endotheliotoxin receptor-Tetramethylpyrazine on the pulmonary arterial hypertension.Methods The64newborn patients were continually main line the tetramethylpyrazine by the mini pump and stimutaneously treated with the respiratory machine.The injec-tion speed was controlled from5μg/kg·min to10μg/kg·min,or to20μg/kg·min.

目的 探讨内皮素受体拮抗剂—阿魏酸钠微量泵持续静脉注射治疗新生儿肺动脉高压的疗效和不良反应方法对64例肺动脉高压的新生儿在呼吸机械通气的情况下同时用微量泵持续静脉注射阿魏酸钠，阿魏酸钠微量泵静脉注射的速度由5μg/开始，在保证体循环血压的基础上可以逐渐加量至10μg/，直至20μg/。

Part at giving drug before (T1), time of zymogen of the blood pressure of the 5 Min(T2) after giving drug, 30 Min(T3), 60 Min(T4) that monitor, serous cruor (enzymatic time of work of cruor of PT), fractionary activation , thrombin , fibrinogen and plaque computation .

分别于给药前(T1)，给药后5 min(T2)、30 min(T3)、60 min(T4)监测血压、血浆凝血酶原时间、部分激活凝血活酶时间、凝血酶时间、纤维蛋白原含量及血小板计数。

Results The pathological characters with mild, moderate and severe spinal cord ischemia/reperfusion injury can be simulated by declamping after 30 min, 45 min and 60 min infrarenal aortic cross-clamping.

结果：阻断腹主动脉血流30、45，60min后开放表现出轻、中、重不同程度缺血再灌注损伤脊髓的病理变化特点。

At the IV dose of 20 and 30 mg/kg, Aloin and Isobarbaloin result appear the mean terminal eliminate half-life（T1/2 β）were 54.1±28.4; 28.7±18.3 and 36.9±21.9;49.7±17.1 min, respectively. The mean area under the serum concentration curve（AUC0-∞）were about 411.655± 13.027;318.213±19.924 and 701.336±116.631;573.278± 86.870μg min/mL, respectively. The mean drug clearancewere 0.165±0.011;0.214±0.016 and 0.149±0.030;0.182±0.035 L/min, respectively.

若静脉注射剂量20和30 mg/kg后，其芦荟大黄苷及异芦荟大黄苷分别平均末端排除半衰期（T1/2 β）为54.1±28.4；28.7±18.3和36.9±21.9；49.7±17.1 min，平均稳定状态分布体积为7.668±3.155；6.343±3.522和5.146±2.490；8.525±2.683 L，平均曲线下面积（AUC0-∞）为411.655 ±13.027；318.213±19.924 和701.336±116.631；573.278±86.870 μgmin/mL，平均血浆中药物最高浓度为23.920±5.669；30.442 ±7.537和57.920±27.690；36.373±14.174 μg/mL，平均药物之清除率为0.165±0.011；0.214±0.016和0.149±0.030；0.182±0.035 L/min。

At the IV dose of 20 and 30 mg/kg, Aloin and Isobarbaloin result appear the mean terminal eliminate half-life（T1/2 β）were 54.1±28.4; 28.7±18.3 and 36.9±21.9;49.7±17.1 min, respectively. The mean area under the serum concentration curve（AUC0-∞）were about 411.655± 13.027;318.213±19.924 and 701.336±116.631;573.278± 86.870μgE min/mL, respectively. The mean drug clearancewere 0.165±0.011;0.214±0.016 and 0.149±0.030;0.182±0.035 L/min, respectively.

若静脉注射剂量20和30 mg/kg后，其芦荟大黄苷及异芦荟大黄苷分别平均末端排除半衰期（T1/2 β）为54.1±28.4；28.7±18.3和36.9±21.9；49.7±17.1 min，平均稳定状态分布体积为7.668±3.155；6.343±3.522和5.146±2.490；8.525±2.683 L，平均曲线下面积（AUC0-∞）为411.655 ±13.027；318.213±19.924 和701.336±116.631；573.278±86.870 μgEmin/mL，平均血浆中药物最高浓度为23.920±5.669；30.442 ±7.537和57.920±27.690；36.373±14.174 μg/mL，平均药物之清除率为0.165±0.011；0.214±0.016和0.149±0.030；0.182±0.035 L/min。

Mm, 5 μm and a UV detector. Elution was performed at a flow rate of 1 mL/min, with a gradient of methanol-acetonitrile-water starting at 80∶10∶10, increasing linearly to 86∶10∶4 in 35 min, then returning to the initial conditions in 5 min. Elution of phenacyl esters was monitored by absorbance at 254 nm. Pentadecanoic acid was used to be an internal standard.

分析所用色谱柱为ZORBAXSB C18柱，以十七酸作为内标，甲醇-乙腈-水为流动相，采用梯度洗脱(梯度洗脱程序为甲醇-乙腈-水由80∶10∶10在35 min内线性变化到86∶10∶4，然后在5 min内恢复到起始比例，流动相流速为1 mL/min)，通过测定苯乙酰甲酯在254 nm处的吸光度值来测定脂肪酸含量。

In the temperature rang from 120 to 150℃, the relations between the apparent reaction rate constant and temperature of the photochlorination in the side chain of m-chloromethyl benzene were: min-1 min-1 min-1 The apparent reaction activation energies are: Ea1=33.54、Ea2=32.45、Ea3=24.26kJ/mol.

在120～150℃温度范围内，间氯甲苯侧链光氯化表观反应速度常数与温度的关系为： min-1 min-1 min-1 表观反应活化能分别为：Ea1=33.54、Ea2=32.45、Ea3=24.26kJ/mol。

Then the experimental rats were treated with posthepatic manipulative bleeding or IH-VC manipulative bleeding at 10 min after reperfusion. RESULTS: MDA concentration in SH-VC elevated obviously with time and peaked at 10 min after reperfusion. Two percent of body weight posthepatic manipulative bleeding with blood transfusion at 10 min after reperfusion significantly decreased circulating MDA, ALT and AST levels and improved survival rate.

结果：HIR时SH-VC血中MDA的含量随时间的延长逐渐升高，并在再灌注后10min达到高峰，并且再灌注后0、5和10min 3个时段，SH-VC内MDA的含量明显高于IH-VC内的含量；2% SH-VC放血输血处理组血清MDA的含量明显低于未处理组，处理后6h血清ALT和AST的含量都显著低于未处理组，并且生存率明显提高达7d左右。

Inoculate a single colony in BMGY medium, grow at 30 °C with 250 r/min until culture reatch an OD6oo=2.0. Harvest the cells by centrifuging at 1,500 r/min for 5 min at room temperature. Resuspend cell pellet to an OD6oo=10 in BMMY medium to start induction.

将经过酶切鉴定和测序正确的阳性克隆电转入毕赤酵母GS115中，挑取酵母转化单菌落，接种于BMGY培养基，30℃、250r/min培养至OD_(600)=2.0时室温1，500r/min离心5min，用BMMY培养基重悬菌体使OD_(600)=1.0，重新放入摇床进行诱导。

I had a lovely dog named PiPi, he was one of my best friends.

我曾经有过一只小狗，它的名字叫皮皮，它很善良，它是我最忠实的朋友。

Newly ovulated mouse oocytes were cultured in the CZB medium supplemented with different concentrations of pyruvate before treated for activation or assayed for MPF activity.

我们猜测卵母细胞在不含丙酮酸的条件下老化的更快，并且卵丘细胞可能通过消耗不含葡萄糖的CZB中的丙酮酸从而促进卵母细胞老化。