查询词典 MIN

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Methods 58 cases patients needing endocardiac orthoptic surgery were enrolled in this study, they are divided into control group and SFI group randomly. Injecting of SFI 1ml/kg by intravenous before CPB was performed in group SFI, tidal volume, Pmax , Pmean and calculating Crs and RI during 10 min , 30 min , 60 min after the trachea intubating, in the beginning of CPB and during 10min, 60min, 120min after CPB were recorded.

选择临床心内直视手术病人58例，随机分为对照组和SFI组，SFI组于CPB前予SFI 1ml/kg静注，记录气管插管后10、30、60 min，CPB手术开始时，CPB停止后10、60、120min时的潮气量、气道峰值压、平均气道压并计算肺顺应性和呼吸指数。

Result The spindles of oocytes were abnormal at 25℃ for 20 min, and disappeared at 25℃ for 30 min or at 4℃ for 10 min. 5, 10, 20 and 40 μg/ml Cytochalasin B for 2 h had no effects on the spindles of the oocytes. The spindles of oocytes were abnormal in 20 μmol/L colchicines for 1 h, and disappeared in 40 μmol/L colchicines for 1 h. The structure of spindles were perfect for 1-12 h when the oocytes were fixed in 4% paraformaldehyde.

结果］卵母细胞在25℃、20 min时纺锤体异常，25℃、30 min或4℃、10 min时纺锤体缺失；5、10、20和40μg/ml细胞松弛素B作用2 h未对纺锤体产生影响；卵母细胞在20μmol/L秋水仙素中作用1 h纺锤体异常，40μmol/L时作用1 h纺锤体缺失；在4%多聚甲醛中4℃条件下，卵母细胞纺锤体结构以固定时间为1~12 h效果较好。

To 5. 0mm/min, and then partridge pea, from 3. 5 to 4. 0mm/min, Utah sweat pea, from 2. 8 to 3. 5mm/min.

草本植物土壤的渗透速度，均明显高于农耕地（2.1～2.3mm/min。

The result showed that the optimal condition of inorgano-absorbent manufacture as following: concentration of vitriol is 15%, the bentonite carrier must be soaked in the 0.4% La(superscript 3+) solution, the pH is 10, microwave radiation power is 340 W for 5 min. The optimal condition of organo-absorbent manufacture process as following: concentration of CTMA is 5%. The rate of wave must 166 W and the time of radiation is 6 mi In addition, the experimental results showed that with inorgano-absorbent the pH number is 3~6 and the reaction time is 45 min, the removal efficiency of phosphor will be up to 99% and the amount of adsorption will be up to 41 mg/g. And with organo-absorbent the pH number is 3~4 and the reaction time is 45 min, the removal efficiency of phosphor is more 30% than that of inorgano-absorbent. The removal efficiency of phosphor will be up to 99% and the number of adsorption will be up to 60 mg/g.

实验表明：①制备无机稀土吸附剂的适宜条件为：硫酸浓度15%，镧浓度0.4%，浸渍pH值10，微波功率340 W，辐射时间为5 min；②制备有机稀土吸附剂的适宜条件为：表面活性剂浸泡浓度为5%，微波功率为166 W，微波辐射时间为6 min；③无机稀土吸附剂当溶液的pH值为3~6，振荡时间为45 min时，对磷去除率为99%以上，吸附量达到了41 mg/g以上，有机稀土吸附剂当溶液的pH为3~4，振荡时间为45 min时，其效果比原来镧改性的无机吸附剂吸附量提高了30%，磷去除率为99%以上，吸附量达到了60 mg/g以上。

The results showed that while the raying distance was 10 cm and an ultraviolet lamp with 20 W acted on the strain of Norcardia corailina 3 min and 4 min, the lethal ratio was high than 90%; While the raying time was 5 min, the lethal ratio was 100%.

结果表明：在紫外灯功率为20w，照射距离为10cm的条件下，诱变时间为3min和4min，致死率均大于90％；而诱变时间为5min，致死率为100％。

Compared with the reference meal, the highest level of vinegar significantly lowered the blood glucose response at 30 and 45 min, the insulin response at 15 and 30 min as well as increased the satiety score at 30, 90 and 120 min post-prandially.

与对照相比，高水平的醋明显降低30和45分钟时的血糖反应和15和30分钟时的胰岛素反应；明显升高餐后30、90和120分钟时饱腹感评分。

In this research, the activities of CWDE were measured by saccharifying according to the theory that red-brown amino compounds were produced when 3, 5-dinitro salicylic acid and aldehyde were heated together. And the testing conditions were made clear. The activity of Cx should be tested with optimal temperature of 50℃, optimal pH of 4.8, incubation time of 30 min, and optimal substrate of 0.6% CMC-Na. Due to the heat-libality, Cx should be sterilized more than 10 min. The testing conditions of the activity ofβ-G were almost the same with Cx except the substrate of 0.5% salicin and the sterilization time of more than 15 min.

主要取得了一下成果：(1)明确了条锈菌细胞壁降解酶活性测定的条件：羧甲基纤维素酶活性的测定条件为50℃，pH4.8，反应时间30min，底物浓度为0.6%羧甲基纤维素钠，以DNS法测定，且失活酶液的灭活时间应大于10min；β-葡萄糖苷酶活性的测定条件为：50℃，pH4.8，反应时间30min，底物浓度为0.5%水杨苷，以DNS法测定，且失活酶液的灭活时间应大于15min；果胶酶活性的测定条件为：50℃，pH4.8，反应时间1h，底物浓度为0.25%果胶，以DNS法测定，且失活酶液的灭活时间应大于10min。

Results: Pigeons exposed to -70°C for 12 min and -90°C for 6 min and 9 min showed slight proliferation of fibrous connective tissue in the perilymphatic space at the site of cryosurgery and presented a selective decrease of the semicircular canal functions.

结果：在-70°C 12 min、-90°C 6 min和9 min显示冷冻处外淋巴间隙纤维组织轻度增生，选择性的半规管功能降低；6例患者经1年4个月至2年5个月随访，5例治疗成功，眩晕消失，1例耳鸣有缓解，4例听力无变化，1例听力下降18 dB。

Pe Min Kam Wan, Lam Kam Sang Nasalin and Wo Chun Tong possess antiallergic activities. Senecio scandens and Selaginella may be the effective ingredients of Wo Chun Tong and Pe Min Kam Wan, and Xanthium sibiricum may also be one of the effective ingredients of Pe Min Kam Wan.

速效鼻敏感丸、和春堂鼻敏清片及林金生鼻敏清胶囊具有抗过敏活性，九里明和卷柏可能为速效鼻敏感丸及和春堂鼻敏清片的有效组分，苍耳子亦可能为速效鼻敏感丸的有效组份之一。

The second is to cut a mere 100 calories from your diet , which is the equivalent of a tablespoon of butter.

第二个就是从你的饮食中减少一百卡路里，只是相当于一大汤匙的黄油。

This is where the Rorschach test comes in.

现在，罗氏测试就出现了。