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Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

Begining with conventional books guard against theft, the paper analyses the burglarproof requests of RFID library administration, discusses some kind of burglarproof modes possibly existing in RFID library. So based on own actual demands the liabrary can ensure library burglarproof mode and select good burglarproof devices to ensure library burglarproof function can be realized.

文章从传统的图书防盗入手，分析了RFID图书馆管理对防盗的要求，探讨了RFID图书馆中可能存在的几种防盗模式，从而帮助图书馆根据自己的实际需求，确定图书馆的防盗模式，选择好的防盗设备，来保证图书馆的防盗功能得以实现。

Under the circumstances,book are becoming a main path of people's obtain and buildingup knowledges,moreover,library naturally a specific location in people's life,this graduation design,I with my locality unit- library empolder backdrop, design this books control system,since books are put in storage to demand and browse.and since library card extend to books move,form the all and the one robotization manage mode, ameliorate our school library manage.

在这种形势下，书籍就渐渐地成为人们获取并增长知识的主要途径，而图书馆就自然而然地在人们的生活中占据了一定的位置，这次毕业设计，我以我所在的单位图书馆为开发背景，设计这个图书管理系统，从图书的入库登记到查询浏览，从借书证发放到图书的借阅，形成了一个整体自动化管理模式，改善了我校图书馆的管理。

Under the circumstances,book are becoming a main path of people's obtain and buildingup knowledges,moreover,library naturally a specific location in people's life,this graduation design,I with my locality unit- library empolder backdrop, design this books control system,since books are put in storage to demand and browse.and since library card extend to books move,form the all and the one robotization manage mode, ameliorate our school library manage.

在这种形势下，书籍就渐渐地成为人们获取并增长知识的主要途径，而图书馆就自然而然地在人们的生活中占据了一定的位置，这次毕业设计，我以我所在的单位图书馆为开发背景，设计这个图书管理毕业设计，从图书的入库登记到查询浏览，从借书证发放到图书的借阅，形成了一个整体自动化管理模式，改善了我校图书馆的管理。

Under the circumstances,book are becoming a main path of people's obtain and building-up knowledges,moreover,library naturally a specific location in people's life,this graduation design,I with my locality unit- library empolder backdrop, design this books control system,since books are put in storage to demand and browse and since library card extend to books move,form the all and the one robotization manage mode, ameliorate our school library manage.

在这种形势下，书籍就渐渐地成为人们获取并增长知识的主要途径，而社区图书馆就自然而然地在人们的生活中占据了一定的位置，这次毕业设计，我以我所在的社区图书馆为开发背景，设计这个图书管理系统，从图书的入库登记到查询浏览，从借书证发放到图书的借阅，形成了一个整体自动化管理模式，改善了社区图书馆的管理。

To use phage display construction antibody library, trabant peptide library, protein mutant library and cDNA library, thus generally using this technology to screen single chain antibody, le...

利用噬菌体表面展示技术可以构建抗体库、随机肽库、蛋白质突变体库和cDNA文库，从而广泛用于单链抗体的筛选、先导化合物的筛选、疫苗的研制等，尤其是在单链抗体、模拟抗原表位的筛选及建立食品安全快速检测体系方面，具有广阔的应用前景。

To discover the originality and national characteristics of ancient China Library Science, and to find the inheritance relationship between modern Library Science and ancient China Library Science, this paper demonstrates the identity of ancient China Documentation and ancient China Library Science and study the their knowledge theory orientation by adducing evidences.

为发掘中国古代图书馆学的原创性与民族特色，疏通中国古代图书馆学与现代图书馆学的因承关系，本文从文献学路径出发，论证了文献学与中国古代图书馆学的同一性，并运用文献举证与&知识考古&的方法，全面考察了中国古代图书馆学的知识论取向。

The process features and shape features of the electronic part are classified and defined, and then the feature model library is built, which enables rapid input of the part information. The rules and methods of stamping process design are summarized to build the stamping process library, so that the stamping process can be designed rapidly and intelligently. Furtherly, the process design rules and methods in process station layout of multi-station progressive die as well as the methods of decompounding and compounding process and the expression method of the process station layout are concluded and summarized, and then the rule library and case library of process station layout are built. Designing the process station layout rapidly is carried out based on rules and cases. At last the rapid design function of process station layout is validated through simulating the rapid designing layout system of multi-station progressive die on Pro/E 3D platform.

主要研究成果有：对电子产品冲压件的工艺特征与形状特征进行了分类和定义，建立了特征模型库，实现了冲压件信息的快速输入；归纳了冲压工艺设计规则和方法，建立了冲压工艺知识库，实现了冲压工艺快速与智能化设计；归纳总结了多工位级进模工步排样中的工序设计原则和方法，工序分解与工序组合方法，工步排样知识的表示方法，建立了工步排样规则库和实例库，利用基于规则和实例的推理方法，实现了工步排样的快速设计；在Pro/E三维软件平台上开发了电子产品多工位级进模工步排样快速设计系统，系统通过实例验证了工步排样的快速设计功能。

The construction of digital library brings a profound revolution to the career of library, and makes the resource construction and operation of digital library digitizes and network from traditional mode a This paper mainly expounds the concept, characters, mode of the digital library, and its construction of basic establishment and information resource and the storage of numerous digital resource.

数字国书馆的建设给图书馆业带来一场深刻的革命，使图书馆的馆藏建设和运作从传统模式向数字化、网络化转变。

County/City public library play a key role in public library system, which share 98% of total public libraries and equip 90% books checking out. The condition of County/City library operation shows the true level of public library undertaking at present in our country.

市县级公共图书馆是我国公共图书馆系统的主体，它们的数量占公共图书馆总数的98％，书刊外借的90％以上是通过市县级图书馆来完成的，市县级图书馆的运营状况很大程度上代表了我国目前公共图书馆的发展水平。

That snake Alaric named Archbishop...

庇护。。。那个毒如蛇蝎的Alaric被命名为大教主。。。

You have done lots of magical things to be amazed at.

你做了很多神奇的事物感到惊讶。