查询词典 HIV

AIM: To isolate bioactive secondary metabolites from the fruits of Schisandra rebriflora Rehd. et. Wils and study their effects on HIV-1 infectivity. METHODS: Inhibition of syncytia formation, HIV-1 reverse transcriptase and protease, protection of HIV-1 infected cells, level of HIV-1 p24 antigen,blockage of fusion were detected. RESULTS: A lignan compound, named rubrifloralignan A, was first isolated as a natural product with modest inhibition effects on syncytium formation and HIV- 1 replication and could protect HIV-1 infected cells with weak inhibition effects on fusion and RT, but it showed no effects on the replication of HIV-1 chronic H9 and PR. CONCLUSION: Rubrifloralignan A is an efficient anti-HIV-1 product with effect on early stage of HIV-1 replication.

目的：从红花五味子果实中寻找活性代谢产物，研究其抗HIV—1活性和作用机制方法：通过合胞体抑制、HIV-1感染细胞保护、HIV—1p24抗原测定、融合阻断、逆转录酶和蛋白酶活性分析等实验，检测分离化合物的抗HIV—1活性并探讨其作用机制一结果：红花五味子甲素能够抑制病毒诱导的合胞体形成，保护病毒感染细胞，抑制病毒在细胞内的复制对HIV-1感染细胞与正常CD4^＋细胞间的融合，逆转录酶活性有一定的抑制作用但是红花五味子甲素不抑制病毒在慢性感染细胞中的复制，也不抑制蛋白酶的活性、结论：首次从天然产物中分离得到红花五味子甲素，它对HIV—1病毒复制早期具有抑制作用

For most viruses,there is aneed for antimicrobials that target unique viral molecular properties.Acycloviris one such drug.It is activated into ahuman herpesvirusDNA polymerase inhibitor exclusively by HHV kinases and,thus,does not suppress other viruses.Here,we show that ACV suppresses HIV-1in HHV-coinfected human tissues,but not in HHV-free tissue or cell cultures.However,addition of HHV-6-infected cells renders these cultures sensitive to anti-HIV ACV activity.We hypothesized that such HIV suppression requires ACV phosphorylation by HHV kinases.Indeed,an ACV monophosphorylated prodrug bypasses the HHV requirement for HIV suppression.Furthermore,phosphorylated ACV directly inhibits HIV-1reverse transcriptase,terminating DNA chain elongation,and can trap RT at the termination site.These data suggest that ACV anti-HIV-1activity may contribute to the response of HIV/HHV-coinfected patients to ACV treatment and could guide strategies for the development of new HIV-1RT inhibitors.

对大多数病毒而言，都需要有针对其分子特性的靶向杀毒剂阿昔洛韦就是这样一种靶向药物在人疱疹病毒酶的特定作用下，阿昔洛韦被激活成为人疱疹病毒DNA聚合酶抑制剂，因此不能再抑制其它的病毒我们的研究发现阿昔洛韦在共感染人疱疹病毒的组织中可以抑制HIV-1，但在无人疱疹病毒感染的组织或细胞中无此作用然而，加入人疱疹病毒-6感染的细胞却使得其对抗HIV的阿昔洛韦变得敏感我们推测这种抑制作用依赖于人疱疹病毒酶导致的阿昔洛韦磷酸化实际上，单磷酸化的阿昔洛韦前体药物无需人疱疹病毒的参与即可抑制HIV此外，磷酸化的阿昔洛韦能直接抑制HIV-1逆转录酶，将其阻止在终止位点，从而终止DNA链的延长这些结果提示阿昔洛韦的抗HIV-1活性决定了艾滋病病毒/人疱疹病毒共感染的患者对阿昔洛韦的治疗反应，也有助于开发新的HIV-1逆转录酶抑制剂

On the establishment of shielding immune cells avoid HIV viral antigens identified protein adsorption and the establishment of "biological trap" induce HIV models Summary : HIV is the human immunodeficiency virus caused AIDS virus, AIDS is not currently be fully human control, AIDS vaccine is being developed, but because the virus genetic diversity potential to change the topic of the complexity of this ban from the incidence of HIV spread T4 cells in the mechanisms by, proposed changes T4 lymphocyte surface protein CD4 receptors structure as to avoid HIV adsorption, and the establishment of "biological trap" booby HIV idea to try to turn on AIDS treatment to the conversion of protein structure and function, as well as synthetic direction.

提要：HIV即人类免疫缺陷病毒是引起AIDS的病毒，目前爱滋病还不能被人类完全控制，AIDS的疫苗也正在研制中，但是由于病毒多样性的遗传潜能，使这一课题变的很复杂，本文试从HIV侵染T4细胞导致发病的机理入手，提出改变T4淋巴细胞表面CD4受体蛋白构象以避免HIV吸附，并设置"生物陷阱"诱杀HIV的构想，试图把对爱滋病的治疗引向对蛋白质结构与功能转化以及合成的方向。

HIV-1B 'strain is endemic in Asia's three major HIV-1 strain of the blood collection in the central region of China's HIV infected persons in the broad popular. B 'strain with other subtypes of HIV-1 strains combine, hastened the birth of a variety of HIV-1 recombinant virus.

HIV-1B'毒株是亚洲流行的HIV-1三大主要毒株之一，在我国中部地区采供血HIV感染者中广泛流行。B'毒株与其他HIV-1亚型毒株结合，催生了多种HIV-1重组型病毒。

The participants were screened for HIV infection and also for recent HIV infection using both the standard test for HIV and a test for HIV that is "detuned" to detect only those HIV infections that have occurred within the six months prior to taking the test.

参与者进行HIV筛查，对那些筛查阳性者还要使用一种称为&detuned&的HIV检测，用以判断是否为检测前6个月内的感染者。198人中，有1例检出了HIV，但不是最近感染的，不能归咎于近期唯一的口交。

Methods Calcein-AM stained H9/HIV-1 ⅢB cells were treated with difierent concentrations of PF in the first,second and third trimesters respectively,which was then mixed with MT2 cells and put under fluorescent microscopy for detection of HIV-1-mediated syncytium formation.MT2 cells were treated with cell-free HIV-1 Ⅲ B,and then washed and cultured with different concentrations of PF in the first,second and third trimesters respectively.Furthcrly,the protecting effect of PFs against HIV-1-infected cells Was determined with MTT,viral replication Was evaluated by measurement of the levels of p24 antigen in culture supernatants with ELISA and the inhibition rate Was calculated.

采用荧光染料Calcien-AM标记的H9/HIV-1 ⅢB分别与早、中、晚孕期不同稀释浓度的PF作用后，与MT2细胞混合培养，荧光显微镜下观察合胞体的形成；用游离的HIV-1 ⅢB感染MT2细胞，并分别与早、中、晚孕期不同稀释浓度的PF作用后，用MTT法检测HW-1感染细胞的存活率，用HIV-1 p24抗原试剂盒检测细胞培养上清中p24抗原含量的变化。

1、It is showed that, health education need to be carried out urgently, widely, deeply and continually in HIV infectors' neighbors and common people in Shanxi Province. 2、Currently, health education to HIV infectors' neighbors and common people should pay more attention to care for HIV infectors, correct misapprehensive and teach prevent measures. 3、 we should take acceptable ways to develop health education, for example: develop affection of media, and dredge hot phone etc. 4、To control HIV infect rate, in the next phase, we should expansion education of safe sex and advocate sex morality.

1、当前，需要在我省感染者周围人群和一般人群中及时、广泛、深入、持久地开展艾滋病健康教育。2、现阶段，我省感染者周围人群的健康教育应注重对感染者的人文关怀、纠正人群对非艾滋病传播途径的误解、开展艾滋病预防措施的教育。3、健康教育应采用易被人群接受的方式，如大力发展媒体作用、开通咨询热线等。4、应积极在各人群中开展安全性行为的教育，提倡性道德，从而控制HIV和其它STD的感染率。5、应努力改善感染者周围人群医疗服务状况，重视感染者及其家属和感染者周围人群的生殖健康需求，为他们提供方便可及的医疗卫生服务。

Objective This study was designed to investigate the different status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons.Methods HIV RNA, HIV DNA were detected with long-distance PCR,reverse-transcription PCR,Southern blot and molecular hybridization from plasma and peripheral blood mononuclear cells, using primers locating between long terminal repeats LTR(U5)-LTR and so on.

HIV是一种变异非常活跃的病毒，这种变异既有可能医疗增强病毒在宿主体内的适应性和生存，又有可能医疗使病毒复制受到阻碍，影响病毒在宿主体内的生存，为了更进一步掌握HIV变异规律，阐明HIV致病机理，我们用逆转录PCR、长片段PCR、Southern转印、分子杂交等对HIV感染无进展者和进展者HIV RNA、CD4细胞、HIV DNA及存在状态进行了研究。

Objective This study was designed to investigate the different status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons.Methods HIV RNA, HIV DNA were detected with long-distance PCR,reverse-transcription PCR,Southern blot and molecular hybridization from plasma and peripheral blood mononuclear cells, using primers locating between long terminal repeats LTR(U5)-LTR and so on.

HIV是一种变异非常活跃的病毒，这种变异既有可能增强病毒在宿主体内的适应性和生存，又有可能使病毒复制受到阻碍，影响病毒在宿主体内的生存，为了更进一步掌握HIV变异规律，阐明HIV致病机理，我们用逆转录PCR、长片段PCR、Southern转印、分子杂交等对HIV感染无进展者和进展者HIV RNA、CD4细胞、HIV DNA及存在状态进行了研究。

The EC_(50) of QKL against the formation of syncytia of MT-2 induced by HIV-1_ was 1/198.02 with a SI of 5.36. By detecting the survival rate of co-culturing H9/HIV-1_ cells and MT-2 cells or MT-2 cell direct infected by HIV-1_ QKL was found to be protective to cells. The EC_(50) was 1/166.67 and 1/144.93 with SI of 4.51 and 3.92 respectively. The EC_(50) of QKL against P24 antigen production was 1/175.44 with SI of 4.75. The drug serum of QKL was also found to be effective to inhibit the cell fusion and protect cells infected by HIV-1_.

结果：QKL对H9／HIV-1_细胞和MT-2细胞的CC_(50)分别为1／50.76和1／36.97；抑制H9／HIV-1_细胞和MT-2细胞早期融合的EC_(50)=1／235.29，SI=6.36；抑制HIV-1_细胞诱导MT-2细胞形成合胞体的EC_(50)=1／198.02，SI=5.36；H9／HIV-1_细胞和MT-2细胞混合培养和HIV-1_感染MT-2细胞时，QKL保护病毒感染细胞免于死亡的EC_(50)分别为1／166.67和1／144.93，SI分别为4.51和3.92；抑制p24抗原产生的EC_(50)=1／175.44，SI=4.75；QKL药物血清也可抑制H9／HIV-1_细胞和MT-2细胞的早期融合，保护细胞免于死亡。

Sodium citrate; calcium carbonate; calcium hydroxide; potassium bicorbonate; magnesium carbonate; ferrous sulphate; Zinc sulphate; copper sulphate; manganese sulphate; potassium iodide; sodium selenite.

柠檬酸钠；碳酸钙；氢氧化钙；碳酸氢钾；碳酸镁；硫酸亚铁；硫酸锌；硫酸铜；硫酸锰；碘化钾；亚硒酸钠。

All this to say that I'm glad filmmaking hasn't become completely ironic. I can enjoy an ironic film as much as the next person, but they usually involve a message of some sort. One that's delivered in a smirkingly clever way that requires little in the way of talent.

所有这些都是说，我很高兴电影制作没有变成完全讽刺艺术，我可以和下一个人一样享受讽刺电影的乐趣，虽然他们经常会被划归到一定级别，技术方面不需要很高的才能。