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In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

I observ'd the poor affectionate Creature every two Minutes, or perhaps less, all the while he was here, turn'd his Head about, to see if his Father was in the same Place, and Posture, as he left him sitting; and at last he found he was not to be seen; at which he started up, and without speaking a Word, flew with that Swiftness to him, that one could scarce perceive his Feet to touch the Ground, as he went: But when he came, he only found he had laid himself down to ease his Limbs; so Friday came back to me presently, and I then spoke to the Spaniard to let Friday help him up if he could, and lead him to the Boat, and then he should carry him to our Dwelling, where I would take Care of him: But Friday, a lusty strong Fellow, took the Spaniard quite up upon his Back, and carry'd him away to the Boat, and set him down softly upon the Side or Gunnel of the Canoe, with his Feet in the inside of it, and then lifted him quite in, and set him close to his Father, and presently stepping out again, launched the Boat off, and paddled it along the Shore faster than I could walk, tho' the Wind blew pretty hard too; so he brought them both safe into our Creek; and leaving them in the Boat, runs away to fetch the other Canoe.

不料星期五力大无比，一下子把那西班牙人背在身上，向小船那边走去。到了船边，星期五把西班牙人朝里轻轻放到船沿上，又把他拖起来往里一挪，安置在他父亲身旁。然后，星期五立即跳出小船，把船推到水里，划着它沿岸驶去。尽管这时风已刮得很大了，可他划得比我走还快。他把他俩安全地载到那条小河里，让他们在船里等着，他自己又马上翻身回来，去取海边的另一只独木舟。我在半路遇上他，问他上哪儿去。他说："去取那只小船。"说完又一阵风似地跑了，比谁都跑得快，甚至可以说比马都跑得快。

In the last chapter, on the basis of theories in paper [4, 5], the notions of strong mixing, weak mixing, generator and expansion of the variable-parametric dynamical system are introduced, it turns out that in variable-parametric dynamical system strong mixing implies weak mixing and then implies transitivity; it is proved that if and both are variable-parametric dynamical system, F conjugates with G , the members of F are communicate with each other and the members of G are also communicate with each other, what's more, they are both homeomorphism, then F is strong mixing implies G has the same properties; futhermore, we prove that F is strong mixing implies F Devaney chaos in the sense of modification in variable-parametric dynamical system and that F Devaney chaos in the sense of modification if and only if G Devaney chaos in the sense of modification when semi-conjugate with and they both are communicate and homeomorphism; at last, we illustrate that F has generator if and only if it has weak generator, and we also prove that if F is expansion, then F has generator.

在第三章中，我们在文[4，5]的基础上，提出了变参数动力系统拓扑强混合、拓扑弱混合以及变参数动力系统的生成子、扩张的概念；证明了变参数动力系统拓扑强混合蕴含拓扑弱混合，进而蕴含拓扑传递；证明了：如果，为两个变参数动力系统，F与G拓扑半共轭，且F两两可交换，G两两可交换，它们均为同胚映射，那么F拓扑强混合，则G也有同样的性质；本章还证明了变参数动力系统拓扑强混合蕴含F在修改的意义下Devaney混沌；在此基础上得出了：如果变参数动力系统与变参数动力系统拓扑半共轭，它们都两两可交换，并且它们均为同胚映射，那么F在修改的意义下Devaney混沌当且仅当G在修改的意义下Devaney混沌；得出了F有生成子当且仅当F有弱生成子；如果F是扩张的，则F有生成子。

This paper will discuss the question of anti-monopoly law's exempt in agricultural area as following: at first, the paper introduces the general theory on exemption from anti-monopoly law; then, this paper will introduce the legislations of United States and European Union on anti-monopoly law's exempt in agricultural area, review and analyze the history, and expound respective jurisdiction and policy; and then, the paper will concretely analyze the legal principle of anti-monopoly law's exempt in agricultural area; and then, it will discuss two concrete questions about system, including defining the concept of"involver"-----the person of legal relation of anti-monopoly law's exempt in agricultural area, and according to the legal frame of anti-monopoly law, the paper will analyze the legitimate action the agricultural cooperative organization; at last, this paper will advance some legislative suggestions about our national Anti-monopoly Act's exempt in agricultural area.

首先，介绍反垄断法豁免问题的一般理论；然后，介绍美国和欧盟在农业领域的反垄断法豁免的立法规定，回顾和分析其立法历史，阐述相关法理和政策考量；其次，具体分析农业领域反垄断法豁免采用的法律原则；再次，主要讨论两个具体制度问题，包括界定"参与人"概念——农业领域反垄断豁免的法律关系的主体，和根据反垄断法框架分析农业合作组织的合法行为；最后，为我国反垄断法农业领域豁免提出立法建议。

A steel bar is washed and dried, and then put into a glass pipe which is sealed to obtain stirring magneton; a mixed solvent of Gamma-methacrylate oxide propyl trimethoxy silane and methanol is prepared and added with an organic base to obtain a pre-bonding solvent; the stirring magneton is immerged into the pre-bounding solvent, and the outer surface of the glass pipe of the stirring magneton is bonded with a layer of silane substance with alkenyl; a reactive monomer mixture and a pore-forming agent mixed solvent are evenly mixed and then added into a container, the stirring magneton after the pre-bounding is put into the container which is sealed and then put into a water bath; after reaction, the container is removed, the stirring magneton coated with a polymer is taken out and put into a extractor and extracted by using a solvent; or the stir bar is immersed into the solvent till no impurity can be checked in the solvent, thereby obtaining the product.

将铁条清洗干燥，放入玻璃管内，密封得搅拌磁子；配制γ-甲基丙烯酸氧丙基三甲氧基硅烷与甲醇的混合溶液，加入有机碱得预键合溶液，将搅拌磁子浸入预键合溶液，在搅拌磁子的玻璃管外壁键合一层带烯基的硅烷化物质；将反应单体混合剂和致孔剂混合溶液混匀后倒入容器中，将预键合后的搅拌磁子放入容器，密封后置于水浴中，反应后除去容器，取出涂有聚合物的搅拌磁子，置于提取器中，以溶剂提取，或将搅拌棒置于溶剂中浸泡至溶剂中无杂质检出，得产物。

The paper analyses material transportation problem and give the solvable methods; analyses delivery vehicle route problem, and then construct its corresponding mathematical model and present a heuristic algorithm based on C—W algorithm; analyses the optimum scheduling problem of trucks, and then construct its corresponding mathematical model and put forward a new algorithm; analyses the Postal Transportation Problem which will perhaps be used in supply chain of manufacturing system, and then construct its corresponding mathematical model and present an approximation algorithm.

分析了制造系统供应链中供需平衡、供需不平衡、有转运等各种情况下的物资调运问题，给出了求解方法；分析了制造系统供应链配送作业中的配送车辆路线问题，建立了相应的数学模型，提出了一种基于C-W算法的启发式算法，该算法较好地解决了有时间约束的配送问题；分析了制造系统供应链中的货运卡车优化调度问题，建立了相应的数学模型，提出了一种基于表上作业法的求解算法；分析了制造系统供应链中有潜在利用价值的邮政运输方式问题，在详细描述了该问题的基础上，建立了相应的数学模型，提出了一种近似求解算法，该算法较好地解决了邮政运输方式这样的多种物流、多种运输方式，有严格时限和众多外部约束的特大型、复杂、并行的交连系统的计划调度问题。

Yet such was the Fright I had taken at the Moors, and the dreadful Apprehensions I had of falling into their Hands, that I would not stop, or go on Shoar, or come to an Anchor; the Wind continuing fair,'till I had sail'd in that manner five Days: And then the Wind shifting to the southward, I concluded also that if any of our Vessels were in Chase of me, they also would now give over; so I ventur'd to make to the Coast, and came to an Anchor in the Mouth of a little River, I knew not what, or where; neither what Latitude, what Country, what Nations, or what River: I neither saw, or desir'd to see any People, the principal thing I wanted was fresh Water: We came into this Creek in the Evening, resolving to swim on shoar as soon as it was dark, and discover the Country; but as soon as it was quite dark, we heard such dreadful Noises of the Barking, Roaring, and Howling of Wild Creatures, of we knew not what Kinds, that the poor Boy was ready to die with Fear, and beg'd of me not to go on shoar till Day; well Xury said I, then I won't, but it may be we may see Men by Day, who will be as bad to us as those Lyons; then me give them the shoot Gun says Xury laughing, make them run wey; such English Xury spoke by conversing among us Slaves; however I was glad to see the Boy so cheerful, and I gave him a Dram (out of our Patroon's Case of Bottles) to chear him up: After all, Xury's Advice was good, and I took it, we dropt our little Anchor and lay still all Night; I say still, for we slept none!

但是，我已被摩尔人吓破了胆，生怕再落到他们的手里；同时风势又顺，于是也不靠岸，也不下锚，一口气竟走了五天。这时风势渐渐转为南风，我估计即使他们派船来追我。这时也该罢休了。于是我就大胆驶向海岸，在一条小河的河口下了锚。我不知道这儿是什么地方，在什么纬度，什么国家，什么民族，什么河流。四周看不到一个人，我也不希望看到任何人。我现在所需要的只是淡水。我们在傍晚驶进了小河口，决定一等天黑就游到岸上去，摸一下岸上的情况。但一到天黑，我们就听到各种野兽狂吠咆哮，怒吼呼啸，不知道那是些什么野兽，真是可怕极了！这可把那可怜的孩子吓得魂飞魄散，哀求我等天亮后再上岸。我说，"好吧，佐立，我不去就是了。不过，说不定白天会碰见人。他们对我们也许像狮子一样凶呢！"佐立笑着说，"那我们就开枪把他们打跑！"佐立在我们奴隶中能用英语交谈，虽然发音不太地道。见到佐立这样高兴，我心里也很快乐。于是我从主人的酒箱里拿出酒瓶，倒了一点酒给他喝，让他壮壮胆子。不管怎么说，佐立的提议是有道理的，我接受了他的意见。于是，我们就下了锚，静静地在船上躺了一整夜。我是说，只是"静静地躺着"，我们事实上整夜都没合过眼。

According to alternation of reorganized-KMT "s class nature what the Comintern and Sdalin defined and KMT "s authentic class nature, we"ll find the inner coincidence between the three, KMT "s class nature what the Comintern defined, Sdalin"s "Three Stages Theory" and errors of "Left deviation in the Agrarian Revolutionary War. They have the same fault: on the theory of Chinese bourgeoisie. Comintern looked on Kuomintang as an organzation of united front, which cut apart the close relationship between Kuomintang and the boureoisie, and then inevitably led to a long-standing mistaken concept, this is, confusing the large boureoisie with the national boureoisie and the petty boureoisie. Therefore, as the large boureoisie betrayed the revolution, Comintern regarded the national boureoisie and the petty boureoisie as the underminer of the organzation of the united front, and then struck a heavy blow at then.

考察梳理共产国际、斯大林对改组后国民党性质的判定及其变化和国民党的真实阶级性质可知，共产国际对国民党性质的判定、斯大林"三阶段"论和土地革命战争时期中共党内的历次"左"倾错误三者之间，前后相续，一以贯之，具有内容上的共通性和逻辑上的连续性，它们共同的要害是共产国际、斯大林有关中国资产阶级理论的错误，一是共产国际混淆在半封建半殖民地中国的带买办性的大资产阶级与民族资产阶级，二是把民族资产阶级的妥协与革命的两面性机械地认定为革命与反革命的"两段性"，认为中国资产阶级必然要叛变革命。

O, she says nothing, sir, but weeps and weeps; and now falls on her bed; and then starts up, and Tybalt calls; and then on Romeo cries, and then down falls again.

啊，她没有说什么话，姑爷，只是哭呀哭个不停；一会儿倒在床上，一会儿又跳了起来；一会儿叫一声提伯尔特，一会儿哭一声罗密欧；然后又倒了下去。

This thesis dedicated to research the investment control of BT project, researched the risk in the program construction, the compose portion and the factors effecting the investment. The research includes the conception and the character of the BT project; the actualize process and the questions should be pay attention to; the conditions of BT contract and the sector of the compose poison of the contract price. In this part researched the conception of the BT purchase price and concluded that the relation between the purchase price and the investment. Then studied the risk factors that effect the investment according to the character of the project and then brought forward several advices of the contract item about how to share the risk. Studied compose sector and it's calculatingly methods of the construction cost which is the main part of the investment, then studied how to control the cost during the construct process and analyzed project variations as well as how control that. In the end, researched the capital cost of BT program, the main content include the concept of the capital cost, the calculatingly methods using the engineering economic method, the method writer suggest in the thesis according to the project practice, how to control the capital cost, mainly through setting up an account and inspecting it, and regulating the program financing rate in reason.

本论文的研究重点即BT项目的投资控制，围绕该问题，本论文的主要研究内容有，从BT模式和BT项目的基本分析开始，研究了BT模式的基本概念、BT项目的运作过程、基本特征；研究了BT项目的合同条件和合同价款，该部分分析了BT项目的合同结构、BT项目的承发包工作范围、BT项目的回购价款组成和确定；根据项目建设特征分析了影响BT项目投资的风险因素，并且提出了在对风险进行分配和分担类合同条款制定时建议；建设工程费用是构成BT投资的主体费用，本文对其各组成部分的基本构成、计算方式进行了分析，研究了实施过程中管理控制和工程变更及变更控制的问题；文章的最后对构成BT项目投资的资金成本进行了探讨，分析了资金成本的概念和形成，运用工程经济学的基本理论分析了资金成本各部分的基本计算方式，依据现有的工程实践情况提出了本文建议的计算方式，最后分析了如何对资金成本进行控制――主要通过设立资金账户对其进行监管以及对项目融资的利率进行合理规定。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。