after this
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此后
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ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.
结果:hMSCs诱导前为纺锤形,诱导后第2天部分细胞即开始发生形变,呈球形或短棒状,1周后胞浆中颗粒增多,约20~30%细胞边缘呈毛刷样变化;hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义,CD90未诱导时表达呈弱阳性,诱导后明显增高(P.05);ANP和CX43在诱导前无表达,诱导后第2周开始表达且表达随时间逐渐增强,但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加,低浓度诱导组低于标准浓度诱导组,组间差异有统计学意义(P.05),G_0/G_1期细胞比例诱导后较对照组显著减少(P.05);β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强,在第6周时均达到高峰,第8周时表达开始衰减,Bcl-2、Bax基因表达呈时间依赖性变化,hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。
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Whereas the sensitivities after 48 h were similar for all media tested (77% for MRSA_ID and ORSA; 73% for C_MRSA and ORSA after enrichment ), the specificities of MRSA_ID (98% after 24 h and 94% after 48 h) and C_MRSA (98% after 24 h and 90% after 48 h) were superior to the specificities of ORSAs (92% after 24 h and 83% after 48 h) and TSB-ORSA (86% after 24 h and 81% after 48 h).
结果所有的培养基在经过48h后得出的敏感性均接近(MRSA_ID 和 ORSA的敏感性为77%; C_MRSA和的敏感性为73%),而特异性则是以MRSA_ID (24 h为98%,48 h 为94%)和 C_MRSA (24 h为98%,48 h为90%)为优,优于ORSAs (24 h 为92%,48 h为83%)和 TSB-ORSA (24 h为86%,48 h为81%)。
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Mosley Im glad youre my maker My Loyalty lies in your hands, youre my breath taker Your body, your kiss is in unknown demand So take command, go Timbo I be the same when it all goes up I be the same when it all goes down Not the first one, open it up I be the last one closin it out Dont know if Ill give you a shot yet Lil Mama Im peepin your style Do I think youre dope enough, yup One way of findin it out The way you came at me, boo Dont care, not afraid Im like Wild Really want it from head to toe Question if she gon let it out Anyway the hour glass go I dont worry anyhow Why dont we see where it go Lets figure it out When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after Dark Owww, Oooohhh Owww Come on SoShy I got a little secret for ya I never sleep when comes the night But everytime I smack my fingers I switch back into the light My moon belong to your sun Your fire is burning my mind Is it love or is it lust Something that I just cant describe Am I the one and only Cause youre the only one It felt so long and lonely Waiting for you to come Its lookin bright and early Im willing to close my eyes This is the unusual story Timbo and SoShy When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after Dark Heyy, Heyy, Heyy, Go Nelly!
莫斯利先生您好我很高兴youre进出口公司我的忠诚度掌握在你们手中,youre我的呼吸接受者你的身体,你的吻是在未知的需求因此,接受命令,去廷博俺是相同的时,所有的上涨俺是相同的时,一切都下降不是第一个,打开它俺是最后一次closin出来 Dont知道病患者给你一个镜头还进出口peepin律妈妈你的风格我认为youre涂料不够,烨一个方法是findin出来你的方式来我,嘘 Dont护理,不怕像野生进出口真的想从头到脚它问她是否坤把它放出但无论如何,沙漏去余请勿担心无论如何为什么dont我们看到它去可以让数字出来当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明,消失在您的wayy Yeahh 在上午的后当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明,消失在您的wayy Yeahh 在天黑后早晨 Owww,Oooohhh Owww 来吧SoShy 我得到了亚一个小秘密我从来不睡觉的时候来晚但是,每次我拍击我的手指我转回到光明我属于你的月亮太阳你的火是我心中燃烧是爱还是欲望一些我公正不能描述我是唯一的原因youre唯一的它认为这样漫长而孤独的等着你来它lookin一大早进出口愿意接近我的眼睛这是不寻常的故事廷博和SoShy 当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明,消失在您的wayy Yeahh 在上午的后当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明,消失在您的wayy Yeahh 在天黑后早晨日Heyy,日Heyy,日Heyy,直接耐莉!
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aft peak:艉[水]舱
艉尖舱 aft peak tank; after peak tank | 艉[水]舱 aft peak; aft peak tank; after peak; after peak tank; after peak water tank | 后桅帆船 aft schooner
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After a long interval: after a long time:经过一段很短的时间
After a little, she came back with a bright skirt.过了一会儿, 她穿... | After a long interval: after a long time经过一段很短的时间 | Yesterday, I met a friend of mine after a long interval. 过了一会儿,她...
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After a long interval: after a long time:过了很长时间
e.g. after a little, she came back with a bright skirt.过了一会儿她穿了... | after a long interval : after a long time 过了很长时间 | e.g. yesterday, i met a friend of mine after a long interval.昨天我碰...