鲎

Objective:The kinetic turbidimetric Limulus test was used in the endotoxin assay of Ligustrazin Hydrochloride injection.

摘 要 目的：应用动态比浊法鲎试验定量测定盐酸川芎嗪注射液中的细菌内毒素含量。

An arthropod of the order Xiphosura,which includes the horseshoe crab and many extinct forms .

剑尾目动物剑尾目中的一种节肢动物，包括鲎及许多已灭绝的节肢动物

An arthropod of the order Xiphosura, which includes the horseshoe crab and many extinct forms.

剑 尾目动物剑尾目中的一种节肢动物，包括鲎及许多已灭绝的节肢动物

Methods: The interference evaluation experiment proves that IVIG exerts strong inhibitions in Bacterial Endotorxin Test. While the interfering factors can't be eliminated by treatments such as dilution and neutralisation, they are liable to be inhibited provided that the IVIG samples are to be diluted by 1:4 with the Dilution Solution Ⅰ.The BET for IVIG preparation diluted by 1:4 with the Dilution Solution Ⅰ is carried out comply with the Chinese Pharmacopoeia(2000 Edition) The lysate with sensitivity of 0.125Eu/ml is used in the test,and the results of the BET are comparable to that of Pyrogen Test carried out in rabbits.

通过干扰评价实验证明IVIG对细菌内毒素检查法有强烈的抑制作用，单纯用简单稀释和调整pH值法无法消除干扰，如果用稀释剂对IVIG作1：4以上的稀释，即可消除样品对该检查法的干扰，利用灵敏度为0.125EU/ml的鲎试剂，将样品用稀释剂稀释4倍，按中国药典（2000版）进行细菌内毒素的检查，结果与家兔热原质试验进行比较。

BPI or equal volume of physiological saline was intra-abdominally given immediately after CLP and 12 hours after CLP respectively (2.5 mg/kg of BPI each time). Plasma endotoxin levels were determined with limulus amebocyte chromogenic assay.

腹腔感染康复脓毒症模型采用盲肠结扎穿孔法，分别在盲肠结扎穿孔后即刻和12 h腹腔内注射BPI各2.5 mg/kg或等容量生理盐水，血浆内毒素采用鲎试验显色定量测定法。

BPI or equal volume of physiological saline was intra-abdominally given immediately after CLP and 12 hours after CLP respectively (2.5 mg/kg of BPI each time). Plasma endotoxin levels were determined with limulus amebocyte chromogenic assay.

腹腔感染脓毒症模型采用盲肠结扎穿孔法，分别在盲肠结扎穿孔后即刻和12 h腹腔内注射BPI各2.5 mg/kg或等容量生理盐水，血浆内毒素采用鲎试验显色定量测定法。

METHODS : Intra-abdominal infection induced sepsis was reproduced by cecal ligation and puncture. BPI or equal volume of physiological saline was intra-abdominally given immediately after CLP and 12 hours after CLP respectively (2.5 mg/kg of BPI each time). Plasma endotoxin levels were determined with limulus amebocyte chromogenic assay.

腹腔感染脓毒症模型采用盲肠结扎穿孔法，分别在盲肠结扎穿孔后即刻和12 h腹腔内注射BPI各2.5 mg/kg或等容量生理盐水，血浆内毒素采用鲎试验显色定量测定法。

We collected venous blood of 4 millilitre from all the children,and quantitative determination blood LPS, LBP, mCD14 by limulus reagent kinetic turbimetric assay, flow cytometry technique and ELISA method.

所有SIRS患儿及正常儿童各取静脉血4ml，分别利用鲎试剂动态比浊定量测定法、流式细胞分析技术、酶联免疫试验进行血液内毒素、mCD14、LBP的定量测定。

Results:The sample solution had approximate two times inhibition to LAL and it could be eliminated by 1∶1 dilution.The results of test for endotoxins of thirty sets of samples were same as that of pyrogens test.

结果：样品原液对鲎试验具有近2倍的抑制作用，经1∶1稀释后可消除。30批样品的内毒素检查与热原检查结果相符。

The test for endotoxins can substitute for the pyrogens test on the condition of the sample using sensitivity 0.25 EU.ml-1 TAL detected and 1∶1 dilution.

甲硝唑葡萄糖注射液经1∶1稀释后，使用灵敏度为0.25 EU.ml-1鲎试剂进行内毒素检查可替代其热原检查。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。