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Endotoxin was detected by using limulus lysate test, and the last two indexes were detected by using radioimmunoassay.

分别用实验改良基质法进行内毒素定量测定,放射免疫方法测定肿瘤坏死因子-α和白细胞介素-6(IL-6)。

To inspect the feasibility of endotoxin detection of metronidazole and glucose injection by limulus test.

目的:考察甲硝唑葡萄糖注射液法内毒素检查的可行性,建立其常规内毒素检查法。

1 The sensitivity of limulus batch to be used shall be determined and confirmed to comply with the requirements before the test.

2.5.4.2.1试验前应该对使用批号试剂的灵敏度,应符合规定。

Objective:The kinetic turbidimetric Limulus test was used in the endotoxin assay of Ligustrazin Hydrochloride injection.

目的:应用动态比浊法试验定量测定盐酸川芎嗪注射液中的细菌内毒素含量。

2 Bacterial endotoxin conforming to work standard: used for calibration limulus sensitivity and test positive control.

2.5.3.2细菌内毒素工作标准品:用于标定试剂灵敏度和试验中阳性对照。

Results The experiments indicate that the bacteria endotoxin contained in the injection have no observed interfering with Limulus reagent.

结果 表明该注射液中所含细菌内毒素对试剂反应无干扰作用。

If exposed to Vibrio cholerae, Limulus blood will coagulates immediately, stopping the reproduction of bacteria.

如一接触到霍乱弧菌,的血液就会立即凝固,令细菌失去繁殖作用。

Objective: To study the practical value of Limulus Lysate Test in patients with Liver cirrhosis asites and changes of endotoxin in asites after use antibiotics.

目的:探讨试验在诊断肝硬化腹水并发自发性细菌性腹膜炎中的应用价值及肝硬化腹水病人抗菌素治疗前后腹水内毒素的变化规律。

1 Bacterial endotoxin conforming to national standard: used for arbitration limulus sensitivity and test positive control.

2.5.3.1细菌内毒素国家标准品:用于仲裁试剂灵敏度和试验中阳性对照。

METHODS Plasma endotoxin of patient and healthy people were detected by turbidimetric assay and gel-clot assay with limulus reagents.

分别用定量动态比浊法和试剂定性凝胶法检测革兰阴性菌感染患者和正常健康对照血浆内毒素含量。

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