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After treatment with the above hormones, the rats were established anesthesia with urethan (1g/kg, i. p.); polyethylene catheters were inserted into the external jugular vein and the common carotid artery for drug administration, and then blood pressure was recorded. The arterial catheter was connected to a pressure transducer, and the blood pressure was displayed on the channel of BL-310 Experimental System of Biological Function through IBM computer. We assessed the changes of resting arterial blood pressure, the pressor responses of angiotensin Ⅱ(Ang Ⅱ, 0.01mg/kg), noradrenaline (NA, 0.01mg/kg) and adrenaline (Adr, 0.016mg/kg). The depressor responses of Ach (0.006mg/kg) and carotid sinus baroreflex were also discussed after treatment with the hormones in OVX rats.

用不同雌激素处理21天后,将动物用25%的氨基甲酸乙酯腹腔麻醉(1g/kg),做颈总动脉插管,通过压力换能器,将血压变化输入计算机,通过BL-310智能型生物信号显示与处理系统的压力测定模块,观察去势鼠及不同雌激素替代治疗大鼠静脉注射血管紧张素Ⅱ(AngⅡ,0.01mg/kg)、去甲肾上腺素(NA,0.01mg/kg)、肾上腺素(Adr,0.016mg/kg)的升压反应以及乙酰胆碱(Ach,0.006mg/kg)的降压反应和压力感受性反射的变化。

Results VA and bone structures of the craniovertebral junction were clearly observed and measured in three-dimensional visualization. The exposure of dens was improved in one third condylectomy. However, the decision to perform condylectomy and its extent should be made based on relationship between abnormal dens and occipital condyle.

结果 三维可视化技术使枕颈结合处椎动脉和骨结构的三维构象得到清晰的观察和测量;入路中磨除1/3枕髁能改善齿状突的显露,但应结合病变状态下齿状突与枕髁等结构的关系决定是否磨除或磨除的范围。

Improvement for the dysmnesia of memory due to chronic blood deficiency in brain of rats induced by ligating the carotid arteries of two sides were observed. Results FBD can improve the dysmnesia of mice induced by scopolamine, NaNO2, and 45% ethanol significantly, also dysmnesia due to chronic blood deficiency in brain of rats induced by ligating the carotid arteries.

结果 FBD对由东莨菪碱所致的小鼠记忆获得障碍、亚硝酸钠所致的小鼠记忆巩固不良和45%乙醇所致的小鼠记忆再现障碍均有明显的改善作用;并可改善大鼠双侧颈总动脉结扎所致的慢性脑部供血不足而产生的记忆障碍。

Methods The mice were treated by ig FBD with the doses of 35, 70 and 140mg/kg for one week continuously. Effect of FBD on dysmnesia of acquired learning of mice induced by scopolamine, dysmnesia of memory retention of mice induced by NaNO2, and dysmnesia of reappearance of memory of mice induced by 45% ethanol were studied.

小鼠ig给予35、70、140mg/kg FBD,连续1周,观察FBD对东莨、莨菪碱所致小鼠记忆获得障碍、亚硝酸钠所致小鼠记忆巩固不良和45%乙醇致小鼠记忆再现障碍的影响,并观察FBD对大鼠双侧颈总动脉结扎所致的慢性脑部供血不足而产生的记忆障碍的改善作用。

Objective To examine the value of Edaravone in the treatment of vascular dementia through ethologic and pathologic changes induced by chronic cerebral ischemiaMethods Wistar rats were randomly divided into sham operated group,model group,edaravone group and duxil groupThe chronic cerebral hypoperfusion models were made by permanent bilateral occlusion of both common carotid arteriesThe memory ability and ultramicrostructure of brain tissue were observedResults The escape latencies in model group were more significantly prolonged than that in the other groups (P<005)But there was no significant difference between edaravone and duxil groupAnd pathologic changes observed by electron microscope in nerve cell of edaravone group and duxil group and model group were fairly light than that in sham operated groupEdaravone group had increased mitochondrium and frontal membranous synaptic vesicle and more productive metabolic activityConclusions Edaravone can lessen injures of hippocampal neurons loss to improve the spatial learning and memory abilitiesAnd it is proved further that edaravone can treat VD

目的 观察依达拉奉对慢性缺血致血管性痴呆大鼠行为学及形态学的影响,探讨依达拉奉在治疗VD中的应用价值。方法实验分假手术组、模型组及都可喜对照组及依达拉奉治疗组,应用双侧颈总动脉结扎方法制备慢性脑缺血大鼠痴呆模型,观察各组大鼠的记忆功能及脑组织超微结构改变。结果缺血组水迷宫表现同对照组、都可喜组及依达拉奉组相比有显著差异(P<005),依达拉奉组与都可喜组相比未见显著差异。同时电镜观察结果也发现依达拉奉组及都可喜组与模型组及假手术组相比神经细胞病理改变较轻,且依达拉奉组线粒体及前膜突触小泡增多,代谢活动较都可喜组旺盛。结论依达拉奉可能通过减轻海马神经元的损伤来改善慢性脑缺血大鼠空间学习记忆障碍,证明依达拉奉对慢性缺血致VD大鼠具有治疗作用。

objectiveto investigate the effect of myricetin dispersible tablets on experimental cerebral ischemia-reperfusion injury in rats and thrombosis formation.methodsexperimental cerebral ischemia-reperfusion injury model was induced by ligation bilateral common carotid arteries. the protective effect of myricetin dispersible tablets was observed by detecting the content of cerebral water, sod and mao activities,mda and la contents.

方法采用双侧颈总动脉结扎法制备大鼠实验性全脑缺血再灌注损伤模型,通过测定大鼠脑含水量,超氧化物歧化酶、单胺氧化酶的活性和丙二醛、乳酸的含量,观察杨梅素分散片对大鼠实验性脑缺血的影响;采用体内静脉血栓形成法,观察杨梅素分散片对体内静脉血栓形成的影响;并研究其对血小板聚集的影响。

MethodsExperimental cerebral ischemia-reperfusion injury model was induced by ligation bilateral common carotid arteries. The protective effect of myricetin dispersible tablets was observed by detecting the content of cerebral water, SOD and MAO activities,MDA and LA contents.

方法采用双侧颈总动脉结扎法制备大鼠实验性全脑缺血再灌注损伤模型,通过测定大鼠脑含水量,超氧化物歧化酶、单胺氧化酶的活性和丙二醛、乳酸的含量,观察杨梅素分散片对大鼠实验性脑缺血的影响;采用体内静脉血栓形成法,观察杨梅素分散片对体内静脉血栓形成的影响;并研究其对血小板聚集的影响。

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