预备

In this study, as a original strain, antagonistic bacterium Ata28 was mutated by UV, UV+LiCl, DES and Microwave irradiation; the very goal of the research is to improve the control efficiency of Ata28 strain against tobacco brown spot and obtain strains advanced both in antagonism and biological characteristics, further provides theoretics and practical experience to the production.

采用紫外线和氯化锂复合诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间10s，15s诱变；通过平板对峙培养，筛选出8个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Ata28，抑菌带最大值达9.7mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Uv1-190、Uv1-199，烟苗经Uv1-190、Uv1-199菌液处理3d后，接种Tbs3的防治效果达51.3％、54.9％，比出发菌株Ata28的防治效果好。

Ata28 strain exhibited marked suppression of Tbs3 strain which is the pathogen of tobacco brown spot on PDA plates, and the Ata28 strain had the good control efficiency of tobacco brown spot.

采用紫外线诱变Ata28菌株时，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间10s，20s，30s诱变；通过平板对峙培养，筛选出15个突变菌株在PDA平板上可明显抑制Tos3的生长，抑菌带高于出发菌株Ata28，抑菌带最大值达9.4 mm；在温室控病中，获得了对烟草赤星病有较好防效的突变菌株Uv-126，烟苗经Uv-126菌液处理3d后接种Tos3的防治效果达52.2％，比出发菌株Ata28的防治效果好。

Ata28 strain was mutated by UV with the most optimum mutant time, including 10, 20, 30s, that was chosen by the trial tests, considering the lethality rate and positive mutation rate of Ata28; it was found that 15 mutant strains were shown antagonism against Tbs3 strain by antagonism test on PDA plate, inhibition zone of mutant strains exceeded the original strain Ata28, and the maximum of inhibition zone reached 9.4mm; the mutant strain Uv-126 was found most effective in tobacco brown spot control and a rate of efficiency control reached 52.2% on tobacco plants three days after bacteria spraying.

采用硫酸二已酯诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间20min，40min，60min诱变；通过平板对峙培养，筛选出17个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Am28，抑菌带最大值达10.5 mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Des-66、Des-50，烟苗经Des-66、Des-50菌液处理3d后，接种Tbs3的防治效果达52.0％、56.6％，比出发菌株Ata28的防治效果好。

O LORD our God, as for all this abundance that we have provided for building you a temple for your Holy Name, it comes from your hand, and all of it belongs to you.

耶和华我们的神啊，我们预备这许多材料，要为你的圣名建造殿宇，都是从你而来，都是属你的。

O LORD our God, as for all this abundance that we have provided for building you a temple for your Holy Name, it comes from your hand, and all of it belongs to you.

耶和华我们的神阿，我们预备这许多材料，要为你的圣名建造殿宇，都是从你而来，都是属你的。

Give a preliminary swing to the arm pitching, as in baseball.

例如在棒球运动中，给胳膊定调做一个预备的旋转。

Methods: Fourty-five mandibular second premolars with single oval-shaped root canal were prepared with step-back technique and filled with warm vertical compaction technique. The teeth were divided into three groups randomly: ProTaper group, Mtwo group and manual stainless group. The operating time was recorded.

45颗具有椭圆形根管的下颌前磨牙经逐步后退法预备、热牙胶垂直加压充填后随机分为3组，分别用ProTaper再治疗器械和ProTaper器械，Mtwo再治疗器械和Mtwo器械，手用H锉和K锉去除牙胶。

Were prepared by stainless steel hand files.

手用不锈钢K型锉预备。

As dock of first phase project each prepare to be advanced in full blast, by company of regular ship of China and foreign countries at present dock of tall outside Shanghai bridge relies on the Shanghai that berth runs to regular steamship service of wire of northwest Ou Hang, already began to carry out integral change divide with a hyphen at the end of a line to move.

随着一期工程码头各项预备紧锣密鼓地推进，目前由中外班轮公司在上海外高桥码头靠泊运作的上海至西北欧航线班轮，已开始实施整体迁移行动。

O Lord our God, in Whom we believe and Whose Name we invoke above every name, grant us preparing for sleep relaxation of soul and body, and keep us from all temptations and dark pleasures.

主我们的上帝，我们信赖你，并呼求你超越一切名号的圣名，当我们预备安眠时，求赐我们灵魂和肉身的休憩，保全我们远离一切幻梦和黑暗的意欲。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。