顶体

In the 111 cases as mentioned above, 5 of them were observed to be non-acrosome and round head; 23 were small acrosome, 83 were normal.

结果111例标本中，5例为无顶体圆头精子，23例为小顶体区精子，83例顶体区正常精子。

Sperm capacitation in vitro and acrosome reaction were premise and basic of IVF. So study on sperm capacitation and AR helps to the development of IVF.

精子体外获能和顶体反应是体外受精的前提和基础，因此对精子体外获能和顶体反应进行研究有助于体外受精技术的深入开展。

Further investigation will potentially provide the practical technologies for the diagnosis of human acrosome reaction deficiency

孕酮具有促进人精子受透明带诱导顶体反应的作用；小鼠透明带溶解液具有诱导人精子产生顶体反应的效果并确定了mZPS诱导人精子顶体反应的量效关系，为建立人顶体反应缺陷症的实用诊断技术提供基础。

During capacitation Ca〓 was present on the outer surface of IAM and in the released acrosomal contents.

精子获能前内存Ca〓主要分布于顶体外膜内侧和顶体内膜与核膜之间，并在获能和顶体反应过程被动员利用；顶体反应精子Ca〓分散存在于释放的顶体内容物中和结合于顶体内膜外侧，可能与顶体酶的活性有关；（9）。

High-density material lying at the circumference of a proacrosomal vesicle was arched with a gap in Solen grandis, but round in Solen strictus. From this gap a proacrosomal vesicle of Solen grandis began to invaginate and deform while it moved to the nuclear front. However a proacrosomal vesicle of Solen strictus did not deform until it reached the nuclear front.

大竹蛏精细胞前顶体囊的高电子密度物质分布在周缘呈一个带缺口的弧形而不是圆形，而长竹蛏前顶体囊周缘的高电子密度物质呈一圆形分布；大竹蛏的前顶体囊是先内凹变形，然后一边变形一边移动，而长竹蛏的前顶体囊是在到达核前端后才开始变形。

Antagonists of GABAA receptor/Cl一channel, picrotoxin and bicuculline,have no effect on the progesterone-induced AR,but they can significantly inhibit the GABA-induced AR in Cl--containing medium.

在含CI一培养液中，GABA*受体拮抗剂picrotoxin和bicuculline不抑制孕酮诱发的顶体反应，而抑制GABA诱发的顶体反应。

While in Cl--deficient medium,picrotoxin has significant inhibitory effect on both progesterone- and GABA-induced AR,both progesterone- and GABA-induced AR are inhibited by the Call channel blocker,nifedipine.

相反在缺CI一培养液中，picrotoxin则可明显地抑制孕酮和GABA诱发的顶体反应。孕酮和GABA诱发的顶体反应均可被Cal+通道抑制剂nifedipine阻断。

To make the wapiti sperm capacitated in vitro, we selected and used the method of sperm-up. By making the rate of acrosome reaction as a main index, and the sperm energy, the survial time and the fertilizated rate of wapiti sperm and hamster mature oocytes as auxiliary indexes, it were carried out to compare the effects of wapiti sperm capacitation in vitro under different temperature, different density of pyruvate sodium, caffeine and heparin and different culture medium.

对马鹿精子，使用上浮法诱导其获能，通过对顶体染色测定精子的顶体反应率并作为主要指标，同时以精子活力、精子存活时间和异种受精率为辅助指标，对精子的获能效果进行评价，以比较不同培养温度、丙酮酸钠浓度、咖啡因浓度、肝素浓度以及培养基对马鹿精子体外获能效果的影响。

A series of technologies were established and applied to this study, which include MR electron microscopy by using mannosylated albumin-colloidal gold (DMA-G10) as probe, modified CBB method for acrosome reaction detecting, improved model of human acrosome reaction induced by mZPS (solubilized mouse zona pellucida), in vitro heterogenic fertilization and affinity cytochemistry, preparation and purification of proteins.

本研究建立了MR的电子显微镜探针——甘露糖基化白蛋白-胶体金及其检测法、移植建立了人精子顶体反应的CBB检测法、改良了mZPS诱导人精子顶体反应实验模型，同时运用体外获能、异种体外受精、亲和细胞化学、蛋白质提纯制备等技术与实验模型，对人精子MR的表达、定位和生理功能加以探讨。

The results were as follows sperm vigor parameters were all decreased in 12 patients, whereas, pattern parameters had no singnificant difference. By SEM, the results were as follows: infertility group acrosomes were much more bigger than the normal ones.

结果发现，不育症精子的运动参数：活率、活动力、平均径速度明显下降，而运动方式参数直线分离度却无明显差异，进一步用扫描电镜观察其头颈部，特别是顶体的超微结构，发现不育症精子顶体较大。

Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行，五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益，这将是我们的荣幸。