靶

The cortisol of group Ⅱ and Ⅲ were degressive with the time. The level of cortisol in all the patients recovered in the first morning of post-operation.

本研究利用依托咪酯这一特，应用靶控输注技术以试图减轻患者围术期的应激反应，现总结如下。

The cortisol of group Ⅱ and Ⅲ were degressive with the time. The level of cortisol in all the patients recovered in the first morning of post-operation.

本研究利用依托咪酯这一特性，应用靶控输注技术以试图减轻患者围术期的应激反应，现总结如下。

Evaluation of the Impact of Insect-resistant Transgenic Rice on the Feeding and Oviposition Behavior of Its Non-target Insect, the Brown Planthopper, Nilaparvata lugens Homptera: Delphacidae

抗虫转基因水稻对非靶標害虫褐飞虱取食与產卵行为影响的评价，陈茂；叶恭银；姚洪渭；胡萃；舒庆尧-66文免费论文库，大学生论文库

Among the target tissues of estrogen is endometrium, which undergoes cyclic proliferation, differentiation and desquamation.

目前认为，细胞内E2的浓度不仅决定于内分泌的合成，而且主要受靶细胞内E2合成和代谢的影响。

The development of Chinese herbal medicine is one of main methods for peasants to increase income in the eastern mountain area of Liaoning.The main medicinal plants in Liaoning Province are schisandra,asarum,dioscorea, panax,gentiana,aconitum,fritillaria,lilium,astragalus,saposhnikovia,atractylodes,polygonatum, clematis,dictamnus,etc.The soils of nearly 20 species of the medicinal plant were studied in this paper.The differences among different medicinal plants were analyzed.The species and quantity of fungi,bacteria and antinomycete in soil of medicinal plants were clear.With the pathogen of main diseases of medicinal plants as target,the strains in the control of pathogen were screened from soil of medicinal plants.

在本研究中，首次以辽宁省主栽药用植物：五味子、细辛、穿山龙、移山参、龙胆、黄花乌头、平贝母、百合、黄芪、防风、苍术、玉竹、威灵鲜和白鲜皮等近20种药用植物土壤为研究对象，应用平板稀释法和磷脂脂肪酸测定法对土壤微生物多样性进行研究，通过研究明确了影响土壤微生物多样性的因素；土壤微生物与药用植物关系；以药用植物土壤微生物为供试菌株，药用植物近年来主要病害为靶标菌，进行生防菌株筛选。

Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.

克隆大鼠NGF、BDNF和NT3基因，构建真核表达载体；3种重组载体分别转染星形胶质细胞，G418筛选后获得阳性克隆细胞进行扩大培养；取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞；用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。

In the TGF β signal transduction pathways,the activated Smad directively interact with those factors to form different Smad/factors complexes which are then translocated from cytoplasm to nucleus where they regulate different TGF β responsive gene expression for its mutifunctional roles in cells.

同时，Smad通过与不同蛋白因子相结合形成复合体调控TGF β靶基因的表达可能是TGF β具有多种生物学功能的分子基础。

In the TGF β signal transduction pathways, the activated Smad directively interact with those factors to form different Smad/factors complexes which are then translocated from cytoplasm to nucleus where they regulate different TGF β responsive gene expression for its multifunctional roles in cells.

同时，Smad通过与不同蛋白因子相结合形成复合体调控TGF β靶基因和表达可能是TGF β具有多种生物学功能的分子基础。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

The results show that, firstly, Stellera chamaejasme L, which rely on their developed axial root system in the 10～40cm soil layer to occupy the competitive priority of volume, length, surface area and so on, in the case of their adaptive faculty to poor surroundings resulting from fruit picking, trampling and overgrazing as well as with the phenomenon of restrained growth among various plants, gradually become the dominant plant in the herbaceous community; secondly, this kind of degraded grassland has not displayed any general characteristic of degradation such as surface exposure, accumulative quantity of vegetation reduced, water loss, soil erosion and so on, therefor, it should be dealt with distinguishingly; thirdly, if"Stellera chamaejasme L Killer"and"Oxytropis ochrocephala Killer"invented by CAS are taken advantage of respectively in the bloom period of Stellera chamaejasme L and at the beginning of florescence of Oxytropis ochrocephala bunge by ultra-low volume spray of 1. 05L/hm〓 and 1. 35～165L/hm〓 dosage separately, the death rates of poisonous herbs may achieve above 95% and 92% correspondently; fourthly, this technology with the characteristics of high work efficiency, exclusive selectivity and convenient operation, is safe to non-target plants and other animals, and has no toxic or side-effect to environment; furthermore, an execution of"Stellera chamaejasme L Killer"spray and a two-year grazing prohibition can be effectively applied on degraded grasslands of"Weed and Poisonous plants Type".

结果表明：狼毒凭借发达的轴根系在10～40cm土层中形成的体积、长度、表面积等竞争优势，以及对采食、践踏、过度放牧所形成的不良环境的适应能力和异株克生现象的存在，逐步成为草群中的优势植物；毒杂草型退化草地没有表现出地表裸露、植物蓄积量减少、水土流失等一般性退化特征，对该类草地的治理应区别对待；应用中科院研制的'灭狼毒'和'灭棘豆'分别在狼毒盛花期和黄花棘豆初花期，以1.05L/hm〓、1.35～165L/hm〓的施用量超低容量喷雾，毒草的死亡率可达到95％、92％以上；该技术具有工效高、选择性专一、灭效高、操作简便，对非靶标植物和其它动物安全，对环境无毒副作用的特点；采用喷施'灭狼毒'+短期禁牧措施，可以有效治理毒杂草型退化草地。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。