雄性动物

Right MCAO was induced in all animals by a 4-0 nylon thread with round tip inserted cranially into right internal carotid artery and maintained for 90 min. HSP70 mono-clone antibody immunocytochemistry was used to detect the changes of HSP70 expression in rat cortex at 6, 12,18,24,48 and 72h reperfusion in conjunction with histopathological analysis of rat brain.

应用HSP70单克隆抗体免疫组化染色检测大鼠再灌注6、12、18、24、48及72h脑皮质HSP70表达的变化；并与病理组织学改变进行对照研究。2.20只雄性新西兰大白兔随机分成对照组（n=10）及葛根素组（n=10），所有动物均阻闭腹主动脉肾下段20min，建立兔脊髓腰尾段缺血模型。

The results indicated that (1) the 147Pm, 141Ce and 147Nd had strong adsorptivity in soil, so they were hard to transfer and easily accumulated in soil or sediment;(2) all the organisms (5 aquaties and 4 eatable plants) tested showed apparent 147Pm concentrating capabilities;(3)the residues of 147Pm, 141Ce and 147Nd were unevenly distributed in the animal's viscera and tissues, higher residues were found in bone, marrow, eye, brain, heart, adipose and testis, showing selective accumulation;(4) with intraperitioneal dosing of Nd or Ce 200mg ·kg -1 apparent inhibition to gonadial hormone secretion in mice was found and with 200 and 800mg·-1 of Ce added in feed, the malformation rate of spematozoon showed higher.

综合研究结果表明： 14 7Pm、14 1Ce和14 7Nd在土壤中具有强的吸附性，而且难以迁移，易于在土壤和底泥中积累；( 2 )供试的 5种水生生物、4种陆生食用植物对14 7Pm均具有明显的富集性；( 3) 14 7Pm、14 1Ce、14 7Nd在动物体内脏器和组织间呈不均匀分布，在骨骼、骨髓、眼、大脑、心脏、脂肪和睾丸中残留量较高，表现了明显的选择性蓄积；( 4 )一次性腹腔注射2 0 0mg·kg-1的Ce和Nd对小鼠性腺激素的分泌有明显的抑制作用，以 2 0 0和80 0mg·-1的Ce随饲料摄入对雄性小鼠精子畸形率明显升高。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Methods: After whole body irradiation with 9.0Gy 60Co γ-rays, the female BABL/C mice were injected via the tail vein with whole bone marrow cells of homogenic male mice to establish bone marrow transplantation model. After 6 months, all mice were sacrificed to get liver tissues.

采用交叉性别骨髓移植模型，雌性BABL/C小鼠接受9.0Gy 60Co源γ射线照射后，经尾静脉注射输入同种系雄性小鼠骨髓细胞，6个月后处死动物，取肝组织标本。

At the same time, the effects and mechanism of pyruvate supplement on body composition and lipometabolism during exercise should be discussed.38 male Wistar rats were selected and randomly divided into 4 groups: placebo control group, training control group, supplement control group and training and supplement group. All rats were in normal diets and drink at random.

动物实验：选取38只健康成年雄性Wistar大鼠，周龄8周，随机分为安慰剂对照组(placebo control，简称PC组)、运动对照组(training control，简称TC组)、补充丙酮酸对照组(supplement control，简称SC组)和补充丙酮酸运动组(training and supplement，简称ST组)。

Methods32 Wistar male rats were used to induce focal cerebral ischemia according to Longa's method.

3 2只Wistar雄性大鼠，按照Longa的线栓法制作局部脑缺血模型，但在放入栓塞线时不结扎翼腭动脉，通过局部脑血流监测、动物行为学评分、脑含水量测定、红四氮唑染色和病理学检查等方法评价该模型的可靠性。

If it is true that the male mice are producing songs, it raises questions about how their sounds develop and whether mice - like birds - are able to learn new sounds, said animal communication expert Peter Marler, a behavioral neurobiologist at University of California, Davis.

如果雄性老鼠真的会&唱歌&的话，那么问题就是它们的声音是怎么发出来的，还有老鼠是不是也像小鸟一样能够学一些新的声音，动物沟通专家彼得·马勒说，他是美国加州大学戴维斯分校行为神经生物学家。

Methods: 24 masculine Meriones Unguiculatus were divided at random into two group: the control group and the ischemia group.

24只健康雄性长爪鼠随机分为正常组对照组（6只）和缺血组（18只），夹闭蒙古沙鼠双测颈总动脉10min诱导前脑缺血后，动物分别存活1天、3天或7天。

Methods 120 SD rats were divided randomly into blank group, model group, Baoliezhi control group and Qianliening granule groups with different doses. The models were established with hypodermic injection of testosterone propionate at 4 mg/kg after orchiectomy, at the same time, these rats were gastric perfused with different drugs respectively for 30 days. The body weight, prostate weight, prostate index, morphological changes, the level of T and E2 were measured. Results The body weight in model group was significantly lower than that in control group (P.05), the prostate weight and index were obviously higher than that in control group (P.01). The prostate weight and index were decreased significantly in Qianliening granule groups with meddle and high doses (P.01), the level of testosterone was increased significantly and E2 was decreased obviously in Qianliening granule group with high doses.

将雄性SD大鼠60只，随机分成空白组、模型组、保列治治疗对照组、前列宁颗粒低、中、高剂量组，采用丙酸睾丸酮皮下注射引起大鼠前列腺增生模型，连续给药30d后，观察6组动物体重前列腺湿重前列腺指数前列腺组织形态血清睾酮雌二醇(E2)水平的变化结果模型组大鼠体重较正常组明显减轻(P.05)，而前列腺重量和前列腺指数明显升高(P.01)；前列宁颗粒中、高剂量组的前列腺重量和前列腺指数明显下降(P.01)；前列宁颗粒高剂量组可明显升高大鼠血清T水平，降低E2水平，使T/E2比例升高。

Methods 62 matured medical fitness male rat were made into radiation proctitis animal model, and respectively coloclysised with western medicine and Chinese crude drug.

成年健康Wista雄性大鼠62只，制作放射性直肠炎动物模型，直肠组织做病理检查，并用免疫组化方法比较肿瘤坏死因子-α的表达。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。