重组体

Methods: Two sets of primers were designed according to the cyclostyle of pcDNA3.1-HBV-YVDD, and the full-length of HBV genome with different enzyme digestin spot was amplified.

以pcDNA3.1-HBV-YVDD为模板设计两对引物，扩增带不同酶切位点的HBV全基因，扩增产物纯化后，经酶切、连接，得到pcDNA3.1-HBV-YVDD重组体。

In this research, a method was established to constructe deficience recombinant system of poliovirus that were composed of two instant expression ways.

在本项工作中，一种利用共转染方法和单转染方法的缺陷型脊灰病毒重组体构建方法得以建立，其包括两种瞬时表达系统。

The construction of the recombinant plasmid carrying shRNA to HPV6bL1 lays the basis for the study of its inhibitive effect on cauliflower excrescence.

经测序，测序结果均与目的序列相同，均符合设计要求。结论 HPV6bL1 shRNA质粒重组体的成功构建为研究尖锐湿疣基因靶向抗体打下基础。

It was identified to be a haploidy recombinant by comparing its colonic characteristics, conodia size and DNA content in conidia with those of its parents.

经菌落形态观察、分生孢子大小及其DNA含量测定，认定它为单倍性重组体。

A further development of these techniques makes it possible to obtain homologous recombinants.

这些技术的进一步发展，使获得同源的重组体成为可能。

Unilateral peripheral inoculation of recombinant ectors on the ibrissal pad territory resulted in an about ninefold increase in proenkephalin A mRNA leels in trigeminal ganglion ipsilateral to the infected side.

重组体载体在单侧末梢初级传入去极化范围内的培养，最终导致感染同侧的三叉神经节脑啡肽前体A 的mRNA水平增加约9倍。

Recombinant adenovirus particles were produced after 293 cells transfected with recombinant Ad genomic plasmid DNA digested with PacI, then they were further amplified in 293 cells.

纯化所得腺病毒滴度约为8×1012pfu/L，当MOI=100时，腺病毒感染HepG1细胞的效率＞75％，Western Blot证实在感染重组体腺病毒的细胞中有相应基因产物的表达。

Pronucleus expression recombinant pMAL-C2X-HBV-X is constructed successfully and with the IPTG induction, the recombinant pMAL-C2X-HBV-X can express the X protein in E. coli JM109, which lays the foundation fur the HBV X protein purification and its biological study.

成功构建了HBV X基因原核表达重组体pMAL-C2X-HBV X；在IPTG的诱导下，该重组体在大肠杆菌中可表达X融合蛋白，为进一步纯化HBV X蛋白和研究其生物学作用奠定了基础。

The deduced amino acid sequence resulted in 53 amino with 46% identity to amphibia.

将阳性重组体进行测序，分析蛙皮素的氨基酸序列，显示它属于蛙皮抗菌肽家族。

An in vitro transcription recombinant plasmid 5T1d was obtained after subcloning the target gene of clone 5T1d,which contained mutant HCV 5 non-coding region cDNA,into a transcriptive vector pCDNA Ⅰ.

采用含HCV5非编码区(5NCR)缺失突变的重组质粒5T1d，将其目的基因亚克隆到体外转录载体pCDNAⅠ多克隆位点上，获得转录重组体5T1d。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。