赤经

Efficacy of Cinnamoum camphora oil, Chinese cinnamon oil and Luicium verum oil which were selected from eight plant volatile oils against the stored grain instcts (Sitophilus zeamais, Rhizopertha dominica ,Tribolium confusum) was studied.

文摘：从天然植物中提取的挥发油经初步试验筛选出8种杀虫效果突出的植物，以黄樟油、肉桂油、八角油对试虫(玉米象、谷蠹和赤拟谷盗)作用方式的测定发现，这些植物挥发油具有强烈的熏杀和触杀作用，而且具有用量低，致死作用迅速等特点。

Efficacy of Cinnamoum camphor a oil, Chinese cinnamon oil and Luicium verum oil which were selected from eight plant volatile oils against the stored grain instcts (Sitophilus zeamais, Rhizopertha dominica,Tribolium confusum) was studied.

文摘：从天然植物中提取的挥发油经初步试验筛选出8种杀虫效果突出的植物，以黄樟油、肉桂油、八角油对试虫(玉米象、谷蠹和赤拟谷盗)作用方式的测定发现，这些植物挥发油具有强烈的熏杀和触杀作用，而且具有用量低，致死作用迅速等特点。

Based on the molecular structures, sugar alcohols can be classified into Mono sugar alcohol (such as erythritol, xylitol, sorbitol and mannitol ) and Disaccharide alcohol (such as maltitol,lactitol and isomaltitol).

糖醇是醛糖或酮糖的羰基经氢化还原成羟基的多元醇；按其结构可分为单糖醇(如四元赤藓糖醇、五元木糖醇、六元山梨糖醇和甘露糖醇)，双糖醇(如麦芽糖醇、乳糖醇、异麦芽酮糖醇)。

Structure Design and Modification of Mannitol-polylactide Tissue Engineering Materials;2. Based on the molecular structures, sugar alcohols can be classified into Mono sugar alcohol (such as erythritol, xylitol, sorbitol and mannitol ) and Disaccharide alcohol (such as maltitol,lactitol and isomaltitol).

糖醇是醛糖或酮糖的羰基经氢化还原成羟基的多元醇；按其结构可分为单糖醇(如四元赤藓糖醇、五元木糖醇、六元山梨糖醇和甘露糖醇)，双糖醇(如麦芽糖醇、乳糖醇、异麦芽酮糖醇)。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

It belongs to fuel processing technology field. It includes the following steps: using alumina industrial waste red mud as material; drying and grinding; adding red mud fine powder into 200-350 screen mesh coal powder by the weight of the former is 10-20% of the latter; mixing uniformly and spraying 5-10% water; forming coal ball by hydraulic press; its pressure is 10-30MPa.

本发明的氧化铝生产厂的废料赤泥为原料，烘干研磨，然后在一定量的200～350目的煤粉中添加上述赤泥细粉，加入量为煤粉重量的10～20％，经共同研磨混合均匀后，喷洒5～10％的水，再次混合均匀，随后用液压机将上述混合物压制成煤球；所用压力为10～30MPa；最终制成具有较高煤炭燃烧率的型煤。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

In this study, as a original strain, antagonistic bacterium Ata28 was mutated by UV, UV+LiCl, DES and Microwave irradiation; the very goal of the research is to improve the control efficiency of Ata28 strain against tobacco brown spot and obtain strains advanced both in antagonism and biological characteristics, further provides theoretics and practical experience to the production.

采用紫外线和氯化锂复合诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间10s，15s诱变；通过平板对峙培养，筛选出8个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Ata28，抑菌带最大值达9.7mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Uv1-190、Uv1-199，烟苗经Uv1-190、Uv1-199菌液处理3d后，接种Tbs3的防治效果达51.3％、54.9％，比出发菌株Ata28的防治效果好。

Ata28 strain was mutated by UV with the most optimum mutant time, including 10, 20, 30s, that was chosen by the trial tests, considering the lethality rate and positive mutation rate of Ata28; it was found that 15 mutant strains were shown antagonism against Tbs3 strain by antagonism test on PDA plate, inhibition zone of mutant strains exceeded the original strain Ata28, and the maximum of inhibition zone reached 9.4mm; the mutant strain Uv-126 was found most effective in tobacco brown spot control and a rate of efficiency control reached 52.2% on tobacco plants three days after bacteria spraying.

采用硫酸二已酯诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间20min，40min，60min诱变；通过平板对峙培养，筛选出17个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Am28，抑菌带最大值达10.5 mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Des-66、Des-50，烟苗经Des-66、Des-50菌液处理3d后，接种Tbs3的防治效果达52.0％、56.6％，比出发菌株Ata28的防治效果好。

In order to express the recombinant peptide of both gp52 and pp150 Oterminal peptides from human cytomegalovirus in the Pichia pastoris, the recombinant plasmids were transformed into Pichia pastor is by electroporation after linearised respectively by Sac I or Bg1II, the transformants were screened in MD plates without histidine.

为了在巴斯德毕赤酵母中表达人巨细胞病毒gp52C末端和pp150C末端串联片段的嵌合肽，将构建好的hCMVp-pPIC9K重组质粒分别经Sac Ⅰ和Bg1 Ⅱ酶切，电打孔法导入毕赤酵母GS115后，在缺组氨酸的MD板上筛选出转化子。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。