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METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. Then 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数来评估神经元的情况。

Result: Schwann-like cells disperse in the hole of nerve graft at oneweek under Scanning Electronic Microscope, the processes of thesatellite-like cells adhere to the wall of graft.There was no statisticaldifference of activity of cells in both group(P>0.05) using MTT. At 12weeks,the result of appearance, histology and TEM were similar, therewere no statistical difference in SFI%, NEP and quantitative analysis ofrecovery rate of myelinated fiber populations, diameter of myelinatedfiber and thickness of myelin sheat.

结果:1w后扫描电镜下可见接种的类Schwann细胞分散在材料表面的孔隙内,细胞形态为星状,细胞突起与支架管壁粘附;MTT法测定细胞活性示神经支架组在各时间点与对照组无显著性差异P>0.05;12w时两组动物坐骨神经外观、组织学光镜观察、透射电镜观察结果相似、两组坐骨神经功能指数恢复率、神经电生理、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率等指标相比无显著性差异(P>0.05)。

Anchorage Mayor Mark Begich "D" defeated Sen. Ted Stevens, ending the tenure of the longest-serving Republican in Senate history, after the counting of more ballots yesterday gave him a larger lead than the number of votes still untallied, Alaska elections officials said.

安克雷奇市长马克·Begich&D&击败参议员Ted史蒂夫,在参议院史上结束服务时间最长的共和党的任期,在计数之后更多选票昨天仍然与票数相比把一比较大领先优势给他untallied,阿拉斯加大选官员说。

Program counts the time from start to finish and stores the best result.

程序计数的时间从开始到完成和商店的最好成绩。

They generally take an intolerable amount of time and are accompanied by innumerable false signals that cause many operators to be whipsawed in and out to an exasperating degree.

它们常常会维持一段令人难以忍受的时间,伴随难以计数的假信号,造成很多交易者惨遭洗盘出场。

Meanwhile when car move, making use of the count function of the SPCE061A interior severance, synchronously display the car's movement time.

同时在小车运动时,利用SPCE061A内部的中断计数功能,同步的实时显示小车运动时间。

After semiquantitative exercise tests and epinephrine tests, WBC, LYM and NEUT have similar timecount curves, so the semi0quantitative exercise test may replace the epinephrine test.

结论半定量运动试验和肾上腺素试验后WBC、NEUT和LYM有相似的时间计数曲线,半定量运动试验有取代肾上腺素试验的可能性。

After semiquantitative exercise tests and epinephrine tests, WBC, LYM and NEUT have similar timecount curves, so the semiquantitative exercise test may replace the epinephrine test.

半定量运动试验和肾上腺素试验后WBC、NEUT和LYM有相似的时间计数曲线,半定量运动试验有取代肾上腺素试验的可能性。

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