裸细胞

Some physicochemical therapy as well as nake hepatocytes transplantation can't correct metabolic disarrangement because of severe hepatic diseases, ALF is characteristic of more complications and easily resulting in multiple organs dysfunction , and even multiple organs failure as well as higher mortality. transplant technique of encapsulated hepatocytes can inhibit immune rejection, and quarantee hepatocytes survival, the technique provides a new therapeutic method for ALF.

由于肝脏功能较复杂，一些利用物理、化学方法及裸肝细胞移植来代替肝脏解毒功能，但不足以纠正严重的肝病所致的代谢紊乱，易致多脏器功能障碍甚至衰竭，并发症多，死亡率高；而微囊化肝细胞移植技术，既可阻断受体的免疫排斥反应，又保证移植的肝细胞在体内长期存活，为急性肝衰治疗提供新的治疗方法。

Gracilis were applied to investigate the effect of proplastid on adaptability, in which such structure was found to be important to the competitiveness during heterotrophic growth in the dark; 2 a new procedure for differential display technology was developed and used to study the influence of plastid on nuclear gene expression, with which it was shown that the loss of plastid could cause remarkable changes in expression of certain nuclear genes.

本文主要有两点突出之处：1）应用裸藻褪色突变株与野生型的生长竞争实验探讨了原质体对于裸藻的适应意义，发现在黑暗环境和异养生长条件下原质体对于细胞的生存竞争有重要作用；2）改进了一种差异显示技术并利用该技术来研究质体的有无对基因表达的影响，证明质体丢失可导致某些核基因表达的显著变化。

Methods Surgical specimens/cell suspensions wore transplanted into the various tissues of BALB/c node mice (subcutis, cellular orthotopic injection into renal capsule, perinephrium, surgical orthotopic implantation into renal capsule). Tumorigenicity and metastasis were subsequently cvaluated. Immunohistochemical analysis was employed to determine the expression of VFGF, bFGF, P16, Bcl-2 and C-met in the metastatic and non-metastatic RCC xenograft.

采用皮下移植法、细胞悬液原位注射法、肾周筋膜内法、组织块原位移植肾包膜下法对BALB/c裸小鼠接种RCC组织块，观察各种方法的成瘤及转移情况，并采用免疫组化方法初步对比分析了VEGF、bFGF、P16、Bcl-2、C-met在裸小鼠体内的转移性和非转移RCC的表达情况。

Phaeocystis cells are separated experimentally form the water samples collected in Bohai Phaeocystis blooms in 2005.Plate and dilution was used to separate and culture the Phaeocystis cells from the Bohai water samples; Its ecological properties of growth was studied in this research; The break effect of ultrasonic and freezing method was compared in the breaking treatment of Gymnodinium spp. cells of different periods.

该研究选用平板法和稀释法对渤海棕囊藻赤潮期间水样中的微藻进行了分离培养实验；系统研究了裸甲藻在实验室条件下的生态学特征；考察了超声波法和冻融法对不同生长时期裸甲藻细胞的破碎效果；运用高效液相色谱对裸甲藻和链状亚历山大藻细胞提取物进行了麻痹性贝毒的分析检测。

The harmful algal blooms has been global questions.In the case of much break out of HAB in China sea this research is done. Under the experiment conditions, the ecological growth properties of Gymnodinium spp.

首先在实验室条件下对裸甲藻的生态学生长特性进行考察，然后从有毒赤潮藻细胞(裸甲藻、链状亚历山大藻)提取毒素，使用高效液相色谱进行定性、定量分析。

But Vitamin and inoculate period have little effects. The HPLC analysis results indicate there is no PSP in Gymnodinium spp., a little PSP of 10.81 fmol /cell in Alexandrium catenella.

HPLC检测分析藻细胞乙酸提取物得到以下结论：裸甲藻细胞内不含有PSP，而亚历山大藻细胞内含有PSP且每细胞含量为10.81fmol／cell，易形成有毒赤潮。

Methods: After the model of transplanted pancreatic cancer was established in nude mice with orthotransplantation of human pancreatic cancer cell line into the pancreas, the ischemia of the right lobe of the pancreas was induced with ligation of the gastroduodenal, inferior pancreaticoduodenal and dorsal pancreatic arteries.

在建立人胰腺癌细胞株裸小鼠胰腺原位移植瘤模型的基础上，结扎荷瘤裸鼠胃十二指肠动脉、胰十二指肠下动脉及背胰动脉诱导胰腺右叶区域性缺血，观察缺血对裸小鼠胰腺移植癌群体增殖动力学及细胞周期动力学的影响。

Results 1. HE: Without the effect of TSA, We found that the tracheal epithelium desquamated after 5-Fu treatment. The residual were trifle nude-nucleus cells distributed intervally on the basement membrane .

在有TSA作用情况下，去除5-Fu作用后0-12小时，基底膜上残留裸核细胞基本没有变化，但是数量稍有增加；24-48小时，裸核细胞消失，细胞成扁平状，细胞核变长，细胞浆伸展覆盖基底膜。

Results The incidence of unlineage-associated antigens expression and "null cell" type was 6.78 % in adult AML.The hematopoietic stem/progenitor were expressed in 75 % of the "null cell" type AL cases.In the 10 cases treated,the complete remissionrate was 40 %,"null cell" tyes had significantly higher expression rates of hematopoietic stem/progenitor and Lower CR tate(P 0.05;P 0.05,respectively),compared with those of "non-null cell" type cases observed during the same period.

结果成年人非系列相关抗原表达和&裸细胞&型的发生率在急性髓系白血病中占6.78%，其中早期干/祖细胞表达阳性率为75%，明显高于同期非&裸细胞&型AL(P.05)。10例患者治疗后完全缓解率为40%，明显低于同期非&裸细胞&型AL(P.05)。

Result In control group, The tail moment in the four xenografts were significantly different (F=9.113, P＜0.01). The tail moment in the four xenografts exposed to irradiation (2Gy, 5Gy, 10Gy and 15Gy), reflected the descending tendency of radiosensitivity described below: lung adenocarcinoma＞neuroblastoma＞esophageal squamous carcinoma＞nasopharyngeal squamous carcinoma.

结果 未照射时4种人癌裸小鼠移植瘤的尾力矩有显著差异(F＝9.113，P＜0.01)。4种人癌裸小鼠移植瘤细胞在不同剂量(2Gy、5Gy、10Gy和15Gy)照射后，未做校正时的TM所反映放射敏感性由高到低的变化趋势依次为：肺腺癌＞神经母细胞瘤＞食管鳞癌＞鼻咽鳞癌。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。