表达性

The common features of imprinted genes are, clustering of multiple imprinted genes in one chromosomal region (around 80%), conservation of imprinting among eutherian mammals, asynchrony of DNA replication of imprinted genes, temporal and spatial regulation of expression of imprinted genes, coding for untranslated RNAs as well as proteins, antisense transcripts may regulate expression of imprinted genes. Once established, the imprinting pattern is stably transmitted through cell division but reset in germ cells of the fetal gonads.

约80%的印记基因呈串出现在染色体上；在哺乳动物品种之间，印记基因具有较高的保守性；印记基因的复制通常表现为不同时性；一些印记基因具有印记遗传的时空性；少数印记基因只转录为mRNA而不翻译成蛋白质；印记基因的反意链通常表达，表达产生具有调节印记基因的作用。

Three education pursues:"lead half pace" express fashion, getting remarkable, high-standard goal , school of educational development; It is a characteristic that the school should run conspicuously to pursue the macro statement of the individual character, the micro statement is to insist students' individual character development; It is health and opening of expressing education to pursue harmoniously.

三个教育追求："领先半步"表达了学校教育发展的前卫性、卓越性、高标准的目标；追求个性的宏观表述是学校要突显办学特色，微观表述是坚持学生的个性发展；追求和谐是表达教育的健康性和开放性。

Fourthly, Job satisfaction has significant effect on three factors of Subjective well-being, and it acts as the mediator between Display positive emotion and Positive affection.

第二，情绪劳动与主观幸福感之间存在显著正相关；表达正向情绪和处理他人负性情绪分别对生活满意度有显著的作用；克制负性情绪和表达正向情绪分别对正向情感有显著的作用，而处理他人负性情绪对正向情感有显著的作用。

Objective: By observing the pathological changes of peripheral facial paralysis rats facial nerve and the positive neurons of nitric oxide synthase in the expression of facial nerve tissue, to contrast the effect of spininess and shallow needling with Spininess and fast Needling in expression of NOS.

目的：通过观察周围性面瘫模型大鼠面神经病理形态学改变以及面神经组织内一氧化氮合酶阳性神经元的表达，对比多针浅刺与多针速刺对周围性面瘫大鼠面神经NOS表达的影响，探讨针刺治疗周围性面瘫的作用机理。

Pem gene is expressed in a time and stage specific manner. Pem transcripts are detectable as early as day 6 in mouse embryonic development, and are abundant in day 7 and 8, but decrease precipitously thereafter. Although Pem transcripts are not detectable in most of adult tissues, they are present in reproductive system. Furthermore, Pem has also shown to be specifically expressed in the proximal cauda and distal corpus regions of the epididymis, the regions where spermatozoa gain forward motility and fertilization competence.

Pem基因的表达具有明显的时间和空间特异性：在鼠胚发育的第6天，即可检测到Pem转录本的表达；第7-8天，Pem表达量达到最高，随后迅速减少；Pem基因在成年组织中一般不表达，但可在生殖组织表达；另外，Pem特异地在附睾近侧尾部和远侧的体部中表达，此处是精子获得运动性和受精能力的地方。

It was found that during all three stages, the expression levels of Dnmt1 mRNA in the epithelial cells of the pregnant mice were significantly lower than those in the pseudopregnant mice (P.05), and the level of Dnmt1 protein expression in the pregnant mice was significantly decreased as compared with that in pseudopregnant mice at the 4-cell stage.

结果 Dnmt1蛋白主要表达于上皮层；妊娠组小鼠各期Dnmt1 mRNA表达水平均显著性低于同期假孕组(P.05)，妊娠组4细胞期Dnmt1蛋白表达水平显著性低于同期假孕组(P.05)。

Cerevisiae have found that bZIP proteins play a role in yeast anti-oxidation. at3g51960, the transcription factor found in plants, is highly homologous to that of yeast. To examine the in vivo role of at3g51960, the overexpression and silencing vectors were constructed respectively. By using flower dip-dye, the binary expression vectors, pCAMBTA3301: at3g51960 and pCAMBTA3301: at3g51960Promoter, as well as vectors pCAMBTA3301: at3g51960-GFP and pCAMBTA3301: at3g51960cDNA were transformed to Arabidopsis by Tumefaciens. The transgenetic homozygous Arabidopsis strains have been derived after screening. Analysis on these strains reveals that strains overexpressing at3g51960 gene have higher level of stress resistance, implying a role of at3g51960 in plant stress regulation.

酵母中发现了bZIP家族的转录因子与酵母的抗氧化性相关，植物中的转录因子at3g51960与酵母中的这一转录因子在空间结构上有很高的同源性，为了验证它在体内的功能，本实验构建了过量表达载体和沉默载体，利用花浸染法把农杆菌携带的双元表达载体，pCAMBTA3301： at3g51960，pCAMBTA3301： at3g51960 Promoter和pCAMBTA3301： at3g51960-GFP及pCAMBTA3301： at3g51960 cDNA转入拟南芥，通过多次筛选已经获得了转基因拟南芥的纯合植株，对获得的转基因植株的表型分析发现，在过量表达at3g51960的转基因拟南芥中对胁迫的抗性有一定程度的变化，因此推测at3g51960可能参与植物的抗性调节。

The reactive polymorphonuclear leucocytes and histioˉcytes were positive for TIAI,but not for Granzyme B;The tumor cell pleomorphism and positive rate of Ki67were sigˉnificantly associated with Granzyme B expression.

背景反应性中性粒细胞和组织细胞常表达TIAI，但不表达GranzymeB，肿瘤细胞异型性、Ki67阳性率与GranzymeB表达显著相关。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

The Northern blot analysis showed that the mRNA of ECP 63 is expressed in embryogenic cells, globular, heart-, and torpedo-shaped embryos, but not in seedlings and non-embryogenic cells, which indicated that ECP 63 gene plays a critical role in somatic embryogenesis.

以ECP63 cDNA全长作探针的Northern分子杂交结果表明，ECP63基因在胚性细胞和不同发育时期的胚状体中高度表达，且表达量基本相等，但在幼苗和非胚性细胞中不表达。表明ECP63基因在体细胞胚胎发生方面起着重要作用。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。