血吸虫病

The levels of schistosome circulating cathodic antigen in serum and urine were detected parallelly by McAb-Dot-ELISA. In 81 acute cases, the positive rates in serum and urine were 96.30% and 66.67%(P＜0.01) but the combined positive (positive in serum and／or in urine) rate reached 100%, while in 109 chronic cases, they were 77.06% and 10.09%(P＜0.01) but the combined positive rate was not increased. In 100 healthy individuals from non-endemic areas, no positives were detected in serum and urine with false positive rate of 0%. The cross reaction rates in serum and urine of 51 cases with clonorchiasis were 9.43% and 0% but all were 0% in 48 cases with ancylostomiasis.

提要 应用McAb-Dot-ELISA平行检测血吸虫病人的血清和尿样(浓缩20倍)中日本血吸虫肠相关趋阴极循环抗原。81例急性血吸虫病患者的血清和尿样的阳性率分别为96.30%和66.67%，而血清和尿样组合检测的阳性检出率提高至100%。109例慢性血吸虫病患者的血清和尿样的阳性检出率分别为77.06%和10.09%，组合检测的阳性检出率没有提高。100份健康人的血清和尿样的假阳性率均为0%。54例华支睾吸虫病患者的血清和尿样的交叉反应率分别为9.43%和0%。48例钩虫病患者的血清和尿样的交叉反应率均为0%。

Each year, 280,000 people die of schistosomiasis, also known as bilharzia or snail fever.

每年有28万人死于血吸虫病，也称为裂体血吸虫病或蜗牛热。

Each year 280,000 people die of schistosomiasis also known as ( bilharzia 住血吸虫) or snail fever.

每年有28万人死于血吸虫病，此病也叫做裂体血吸虫或蜗牛热。

Objective To reduce the cost of snail control on the high risk areas with schistosomia infection and to reduce the cost in the prevention of the infection by cercaria, we aim to develop a new method to kill cercaria with the niclosamide-containg sawdust ball.

目的为降低血吸虫病易感地带的灭螺成本，防止人群感染血吸虫病，根据血吸虫尾蚴聚集于水面的生态特点，研制锯木屑氯硝柳胺缓释剂用于灭蚴的新方法。

Methods In a mountainous endemic region of schistosomiasis in Yunnan Province, random samples of the villagers aged 10 - 70 years from three natural villages were examined by using the modified Kato-Katz's quantitative thick smear technique for parasitological stool examination, and the sera from the villagers were tested with the kits of DDIA, ELISA and COPT for immune examination respectively.

方法在云南省大山区型血吸虫病流行区，选择3个自然村作为调查现场，随机抽取10-70岁的村民作为调查对象，进行血吸虫病病史个案调查；收集粪便标本，采用改良 Kato-Katz法(1送3检)作病原学检查；静脉采血，分离血清，分别采用DDIA诊断试剂盒、酶联免疫吸附试验诊断试剂盒和环卵沉淀试验诊断试剂盒进行血吸虫病免疫学诊断。

The present situations of the bovine schistosomiasis in Jingzhou Hubei,were investigated with serological diagnosis;afloating the schistosome eggs with saturated brine;and hatching of miracidium.

日本血吸虫病在我国流行历史悠久，据湖南长沙马王堆西汉女尸与湖北江陵西汉男尸体内发现血吸虫卵的事实，证明2 100余年前我国长江流域已有日本血吸虫病流行。

National Institute of Parasitic diseases,Chinese Center for Disease Control and Prevention,Shanghai 200025,China

中国疾病预防控制中心寄生虫病预防控制所，世界卫生组织疟疾血吸虫病和丝虫病合作中心，中国疾病预防控制中心寄生虫病预防控制所，世界卫生组织疟疾血吸虫病和丝虫病合作中心，上海 200025

The serum samples were detected by Indirect ELISA and Indirect hemagglutination test as reference method to assess the value of the recombinant protein in serodiagnosis.

结论1、大量表达并纯化了重组蛋白SjRPS4，并证实其具有良好的免疫反应性，能与日本血吸虫病阳性血清特异性结合，可应用于日本血吸虫感染血清学诊断。2、成功建立了以重组蛋白SjRPS4为包被抗原的间接ELISA法，在日本血吸虫病的免疫诊断中具有良好的应用价值。

We have obtained the anti-SEA IgY antibody, established and optimized the double antibody sandwich ELISA system to detect SEA of S. japonicum from the sera of acute and chronic patients with schistosomiasis and normal persons. The cross-reaction with sera of paragonimiasis, clonorchiasis and hookworm infections were also investigated. Our results demonstrated that the IgY-ELISA method was effective in evaluating the therapeutic effect in different parasitic burden of infected mice, which provides a novel technique for clinical diagnosis of S. japonicum infection.

本研究成功制备并鉴定了抗SEA的IgY，进行了理化性质的研究，成功建立且优化了IgG-IgY ELISA检测体系，用于正常人，急性血吸虫病患者血清和慢性血吸虫病患者血清的检测，以及肺吸虫、华支睾吸虫、钩虫患者血清的交叉反应性的分析，对感染血吸虫的小鼠进行了疗效考核，为血吸虫病的诊断提供了有效的辅助诊断方法。

Parasitological examinations were conducted for the infections of soil-transmitted nematodes, cestodes and Schistosoma japonicum with Kato-Katz technique and miraci...

对土源性线虫、绦虫和血吸虫感染等采用病原学检查，旋毛虫病、猪囊尾蚴病和血吸虫病采用血清学检查。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。