获得大成功

Biological Qualcomm reported that since the world recognized the success of the most promising AIDS vaccine trial has been declared a failure, the date of successful AIDS vaccine seems to have become the foreseeable future, the Nobel Medicine Prize, the world's leading AIDS expert David Baldi Professor Moreton speech, claiming that the complexity of HIV based on the variability in the 25 years of human AIDS vaccine can not be found,"and most likely never find such a vaccine."

生物通报道，自从被世界公认最有希望获得成功的艾滋病疫苗试验宣布失败以来，艾滋病疫苗成功之日似乎变得遥遥无期，诺贝尔医学奖得主、世界著名艾滋病研究专家大卫·巴尔迪摩教授发表演说，声称基于艾滋病病毒复杂的变异性，人类在25 年内不可能找到艾滋病疫苗，"并且很有可能永远也找不到这种疫苗。"

Fourth, we have effectual method to control the pressure, if we are unwilling to do one's best to lighten the pressure, strengthening motive force, we bring the limitless agony to oneself and others, without success of accomplishing in one move, if we can succeed in dealing with the pressure in life , we may just get oneself and encourage .

第四，我们有控制压力的行之有效的方法，如果我们不愿尽最大的努力减轻压力，增强动力，我们就回给自己及他人带来无限的痛苦，没有一蹴而就的成功，如果我们能够成功处理生活中的压力，我们才有可能获得自我激励。

No He won't have it , he knows his whole back city's ropes It don't matter, he's dope He knows that, but he's broke He's so stacked that he knows When he goes back to his mobile home, that's when it's Back to the lab again yo This whole rap shit He better go capture this moment and hope it don't pass him You better lose yourself in the music, the moment You own it, you better never let it go You only get one shot, do not miss your chance to blow This opportunity comes once in a lifetime yo The soul's escaping, through this hole that it's gaping This world is mine for the taking Make me king, as we move toward a, new world order A normal life is borin, but superstardom's close to post mortar It only grows harder, only grows hotter He blows us all over these hoes is all on him Coast to coast shows, he's know as the globetrotter Lonely roads, God only knows He's grown farther from home, he's no father He goes home and barely knows his own daughter But hold your nose cuz here goes the cold water His bosses don't want him no mo, he's cold product They moved on to the next schmoe who flows He nose dove and sold nada So the soap opera is told and unfolds I suppose it's old potna, but the beat goes on Da da dum da dum da da You better lose yourself in the music, the moment You own it, you better never let it go You only get one shot, do not miss your chance to blow This opportunity comes once in a lifetime yo No more games, I'ma change what you call rage Tear this mothafuckin roof off like 2 dogs caged I was playin in the beginnin, the mood all changed I been chewed up and spit out and booed off stage But I kept rhymin and stepwritin the next cypher Best believe somebody's payin the pied piper All the pain inside amplified by the fact That I can't get by with my 9 to 5 And I can't provide the right type of life for my family Cuz man, these goddam food stamps don't buy diapers And it's no movie, there's no Mekhi Phifer, this is my life And these times are so hard and it's getting even harder Tryin to feed and water my seed, plus See dishonor caught up bein a father and a prima donna Baby mama drama's screamin on and Too much for me to wanna Stay in one spot, another jam or not Has gotten me to the point, I'm like a snail I've got to formulate a plot fore I end up in jail or shot Success is my only mothafuckin option, failure's not Mom, I love you, but this trail has got to go I cannot grow old in Salem's lot So here I go is my shot.

无 他不会有，他知道他的整个回城的绳索它不处理此事，但他的涂料他知道，但他的突破他的，所以堆叠他知道当他回到他的手机在家，就什么时候，它的回到实验室又摇摇这整个说唱为Shit 他最好去捕捉这一刻，并希望它不通过他您更好地失去了自己在音乐，时刻你拥有它，你最好永远不会放手你只能获得一杆，千万不要错过你的机会，以打击这个机遇而来的一生中只有一次的心灵灵魂的逃亡，透过这个洞，它的溃烂这个世界是矿井为录取令我国王，因为我们走向一个新的世界秩序过正常的生活，是borin ，但superstardom的接近邮政砂浆它不仅日益困难，只有长大白热化他的打击我们所有这些锄头，是所有对他的海岸到海岸的表演，他知道作为globetrotter 寂寞的道路，只有上帝知道他的成长到离家远，他的父亲并没有他回家，勉强知道自己的女儿但对于你的鼻子cuz这里有云冷水他的老板不希望他没有谟，他的冷战产物他们转移到下一schmoe谁流向他的鼻子鸽派和出售纳达所以肥皂歌剧是告诉，并逐步落实我想它的旧potna ，但在街上巡逻下去大达达姆弹达达姆弹达大您更好地失去了自己在音乐，时刻你拥有它，你最好永远不会放手你只能获得一杆，千万不要错过你的机会，以打击这个机遇而来的一生中只有一次的心灵没有更多游戏，我是改变你们称之为愤怒催泪这mothafuckin屋顶起飞一样二日犬笼我是playin在beginnin ，心情，一切都变了我是咀嚼起来，吐出一片嘘声起飞阶段但我一直rhymin和stepwritin未来的CYPHER 最好相信有人的payin该pied伯尔所有的痛苦内扩增事实我不能得到我的9至5月我不能提供适当类型的生命，因为我的家庭 cuz男子，这些goddam粮票不买尿布它的不看电影，有没有mekhi phifer ，这是我的生命这些时代，所以很难和它变得更难 tryin到饲料和水我的种子，加上见荣辱赶上了bein父亲和表面唐娜宝宝妈妈戏剧的尖叫和太多，我想留在现场的一个，另一个果酱或不得到了我的一点，我想蜗牛我必须制订一项阴谋前列我结束了在监狱或打针成功是我唯一的mothafuckin选择，失败的不是妈妈，我爱你，但这个线索已经去我不能老在塞勒姆的地段所以在这里，我去的是我的了一枪。

Our partnership with Keiretsu Forum blends the Keiretsu Forum global community of investors and portfolio companies with an established and successful model to foster businesses in larger markets and give them a greater chance of success.

我们与 K4论坛的合作将投资人会员并入其中，以建立并获得成功的模型来帮助其他企业在更大的市场中孕育产业并且提供更大的成功机会。

The results show that increasing the solids loading of slurry up to 45% allows minimizing deformation of green sheets during drying and sintering process effectively. When the content of monomers in shurry is 2%-4%, and the content of plasticizer in shurry is 3%-6%, green sheets with good strength and flexibility are obtained. The microstructures and the electric characteristics of PTCR chip thermistors were investigated. A five-layer chip PTCR thermistor with room resistance of 0.8Ω, temperature coefficient of resistance of 13.40%/℃, and ratio of maximum to minimum of resistance larger than 10^5 is successfully fabricated.

研究表明：浆料固相体积分数对坯体的乾燥及烧结行为有较大影响，当浆料固相体积分数在45%以上时，可有效避免制品乾燥和烧结过程中收缩过大而产生的变形开裂缺陷；当有机单体的质量分数为2%~4%，丙三醇的体积分数为3%~6%时，可获得有一定强度和柔韧性的生坯；研究了注凝成型PTCR陶瓷的微观结构及陶瓷元件的PTCR性能，成功地制备了层数为5、室温电阻为0.8Ω、电阻温度系数为13.40%/℃、升阻比大於10^5的多层片式PTCR元件。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Huang Shan is sufferring the first accident after annihilative blow, want to learn namely, he should be walked out of, seeing those true old bosses is how to score a success, how to defend a success again.

黄山在遭受毁灭性打击后的第一件事，就是要去学习，他要走出去，看看那些真正的大老板们是怎样获得成功，又是怎样守住成功的。

Culture and immunohistochemistry of Schwann cells Schwann cells that are derived from sciatic nerve of Sprague-Dauley rats are successfully isolated, cultured. The Schwann cells are transplanted into the hemisected adult rat spinal cord after purification and condense and enclosed by L I -antibody. Eight weeks later, the experiments of the S- 100 immunohistochemistry indicate that Schwann cells are still alive and array orderly relatively.

本实验的主要研究内容及结果如下： 1、雪旺细胞的培养及免疫组化染色利用新生S-D大鼠的坐骨神经成功地分离、培养获得了纯度较高的雪旺细胞，并将雪旺细胞移植进入大鼠脊髓损伤创面，饲养8周后经过S-100免疫组化ABC染色发现雪旺细胞在大鼠体内成活良好并成簇排列。

How when we are in a Doubt or Hesitation, whether to go this Way, or that Way, a secret Hint shall direct us this Way, when we intended to go that Way; nay, when Sense, our own Inclination, and perhaps Business has call'd to go the other Way, yet a strange Impression upon the Mind, from we know not what Springs, and by we know not what Power, shall over-rule us to go this Way; and it shall afterwards appear, that had we gone that Way which we should have gone, and even to our Imagination ought to have gone, we should have been ruin'd and lost: Upon these, and many like Reflections, I afterwards made it a certain Rule with me, That whenever I found those secret Hints, or pressings of my Mind, to doing, or not doing any Thing that presented; or to going this Way, or that Way, I never fail'd to obey the secret Dictate; though I knew no other Reason for it, than that such a Pressure, or such a Hint hung upon my Mind: I could give many Examples of the Success of this Conduct in the Course of my Life; but more especially in the latter Part of my inhabiting this unhappy Island; besides many Occasions which it is very likely I might have taken Notice of, if I had seen with the same Eyes then, that I saw with now: But 'tis never too late to be wise; and I cannot but advise all considering Men, whose Lives are attended with such extraordinary Incidents as mine, or even though not so extraordinary, not to slight such secret Intimations of Providence, let them come from what invisible Intelligence they will, that' I shall not discuss, and perhaps cannot account for; but certainly they are a Proof of the Converse of Spirits, and the secret Communication between those embody'd, and those unembody'd; and such a Proof as can never be withstood: Of which I shall have Occasion to give some very remarkable Instances, in the Remainder of my solitary Residence in this dismal Place.

有时，我们会陷入无所适从的境地，踌躇不定不知道该走哪条路才好。这时候，内心常常会出现一种暗示，指示我们走这条路，虽然我们原来想走的是那条路。不仅如此，有时我们的感觉、愿望、或我们的任务明明要我们走那条路，可是心里忽然灵机一动，要我们走这条路；这种灵机也不知道是从哪里来的，也不知道出自什么影响，可就是压倒了原来的一切感觉和愿望，使我们走这条路。结果，后来的事实证明，如果我们当初走了我们自己想走的路，或者走了我们心目中认为应该走的路，我们则早已陷于万劫不复的境地。反复思索之后，我自己定下了一条规矩：每当自己心里出现这种神秘的暗示或冲动，指示我应做什么或不应做什么，我就坚决服从这种神秘的指示，尽管我不知道为什么该这么做或该这么走，我知道的只是心里的这种暗示或冲动。在我一生中，可以找出许许多多这样的例子，由于我遵循了这种暗示或冲动而获得了成功，尤其是我流落到这个倒霉的荒岛上以后的生活，更证明了这一点。此外还有许多例子。当时我若能用现在的眼光去看待，是一定会意识到的。但是，世上有许多道理，只要有一天能大彻大悟，就不算太晚。我奉劝那些三思而后行的人，如果在他们的生活里，也像我一样充满了种种出乎寻常的变故，或者即使没有什么出乎寻常的变故，都千万不要忽视这种上天的启示，不管这种启示是什么看不见的神明发出的。关于这一点，我不准备在这里讨论，也无法加以阐明。但这种启示至少可以证明，精神与精神之间是可以交往的，有形的事物和无形的事物之间是有神秘的沟通的。而且，这种证明是永远无法推翻的。关于这一点，我将用我后半生的孤寂生活中一些很重要的例子加以证明。

In 2005 was the successful launch of "sea cat" brand series of poles of the experience has brought in big-renowned brand names in North America AMUNDSON, for two consecutive years through the operation of the domestic market, has successfully put in nine series, including the freshwater , the sea, of which more than 300 species of freshwater-foot wooden pole series has become a vast number ofrelatives and friends gifts gift of choice; sea-pole in the flying fish series, Treasures series has become a best-selling across brands.

在获得2005年成功推出&海猫&品牌系列钓竿的经验之后，有大手笔引进了享誉北美的著名品牌AMUNDSON，通过连续两年的国内市场运作，已成功投放九大系列，包括淡水、海水、300多个品种其中淡水竿的尺木系列已成为广大钓友馈赠亲友的首选礼品；海竿中的飞鱼系列、雄风系列已经成为畅销大江南北的知名品牌。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。