药

To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量；薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用，确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化，流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

Consider to make clear in recent years, second acid is calcic - Bowman does not move bacillus clinical bacterium of single born of the same parents of the detached rate that go up and verdigris holiday comparatives, have the tendency that year after year rises, the fundamental condition pathogenic bacteria that already became the infection inside the courtyard [1] , have to a variety of antibacterial content be able to bear or endure higher property of a medicine [1, 2] , for this, we are monitored and statistic analysed my courtyard to came 1998 2004 7 years a second acid is calcic - the clinical infection condition that Bowman does not change bacillus, to 14 kinds of antibacterial content be able to bear or endure property of a medicine and be able to bear or endure medical tendency, sum up a report to be as follows now. 1 material and method all data all come from 1.1 basic materials to came on January 1, 1998 my courtyard outpatient service was mixed on December 31, 2004 the specimen sending check of inpatient, via clinical microbiology the lab is fostered and classics appraisal is second acerbity calcium - Bowman does not move bacillus to affect case of illness, with clinical and commonly used antibacterial at the same time content makes the kill that medical quick detects. 1.2 bacteria appraisal is mixed medical quick experiment uses live thing VITEK32 of Mei Li dust is full automatic the bacterial appraisal of bacterial analysis system and its form a complete set gets stuck and medical quick card undertakes detecting, 1998~2000 year use GNI appraisal gets stuck, 2001~2004 uses GNI+ card.

近年探究表明，乙酸钙-鲍曼不动杆菌临床上的分离率和铜绿假单胞菌相当，并有逐年上升的趋向，已成为院内感染的重要条件致病菌[1]，对多种抗菌药物有较高耐药性[1，2]，为此，我们监测并统计分析了我院1998年至2004年七年间乙酸钙-鲍曼不动杆菌的临床感染情况，对14种抗菌药物的耐药性及耐药趋向，现总结报道如下。1材料和方法1.1基本资料所有资料均来自于1998年1月1日至2004年12月31日我院门诊和住院病人的送检标本，经临床微生物学实验室培养并经鉴定为乙酸钙-鲍曼不动杆菌感染病例，同时以临床常用抗菌药物作药敏检测的结果。1.2细菌鉴定和药敏试验采用生物梅里埃VITEK32全自动细菌分析系统及其配套的细菌鉴定卡和药敏卡进行检测，1998~2000年使用GNI鉴定卡，2001~2004使用GNI+卡。

The result indicated: antibacterial concentration of inner thallus increased progressively with time prolongation when added CCCP with antibacteria, proved that multi-resistance of E.coli in livestock and avian is mediated really by active efflux system; The multi-resistant level have positive correlation with AcrAB transcription and expression level; Quantitive RT-PCR,Western-blotting and fluorescence quantitive PCR can use to detect AcrAB.these results provide theory foundation to detect and study multi-resistance of E.coli in veterinary clinic and have a important scientific significance to develop neotype antibacterials.

结果表明，多重耐药株在有CCCP 存在时，菌体内抗菌药物的浓度随时间的延长而递增，证明畜禽大肠杆菌多重耐药性确实由主动外排系统介导；其多重耐药水平与AcrAB的转录、表达水平呈现正相关；定量RT-PCR、Western-blotting、荧光定量PCR可用于AcrAB水平的检测。这些结果对检测临床大肠杆菌的多重耐药水平、对研究兽医临床大肠杆菌多重耐药机制提供理论依据，对开发新型抗菌药物有重要的科学意义。

Firstly,we isolated clinically and induced artificially multi-resistant Escherichia coli,detected antibacterials intake by different resistant level strains with CCCP and fluorescence spectro method and ascerained existing active efflux system in this strains; Secondly, construced internal standard of quantitive RT-PCR and detected AcrAB mRNA level of different resistant E.coli with quantitive RT-PCR; Thirdly, prepared AcrA antibody and detected AcrAB protein level of different resistant E.coli; eventually,designed and constructed Taqman probe of AcrAB and detected AcrAB level of different resistant E.coli with fluorescence quantitive PCR.

临床分离及人工诱导大肠杆菌多重耐药株，氰氯苯腙结合荧光分光法检测不同耐药水平菌株对抗菌药物的摄入，确证主动外排系统的存在；构建了定量RT-PCR的内标准DNA，通过定量RT-PCR检测动物源性大肠杆菌AcrAB mRNA的水平；制备AcrA抗体，采用Western-Blotting检测不同耐药株AcrAB蛋白的表达水平；设计和构建AcrA、AcrB的Taqman探针，检测不同耐药菌株AcrAB的水平。

DATA SYNTHESIS: 11 literatures include experimental group and control group, patients in the experimental group were treated with analgetic combined with psychological intervention and antidepressant while those in the control group were only given analgetic; the outcomes of the two groups were compared.

资料综合：11篇文献均包括观察组和对照组，其中观察组应用镇痛药合并心理治疗及抗抑郁药，对照组应用镇痛药但未合并心理治疗及抗抑郁药，对两组患者的疗效进行比较分析。

Results The consumption sum of the drugs for antiacid and peptic ulcer diseases increased year by year,whose average annual incremental rate was faster than that of the west medicine,i.e.25.77%and 12.02%...

结果该院抗酸药及治疗消化性溃疡病药的销售金额逐年增长，其增长率较西药总体增长率高，平均年增长率分别为25.77%和12.02%；质子泵抑制剂和胃黏膜保护剂的销售金额排序各年均位于抗酸药及治疗消化性溃疡病药的前2位；磷酸铝凝胶在3年中均占据用药金额的首位，奥美拉唑的用药频度增长显著，平均年增长率为43.8%。

Results Hela/MMC cells possessed the ability of 5.02 fold resistance to MMC and 2.03 fold cross-resistance to Capsulation and maintain the sensitivity to vincristine, adriamycin and 5-flurouracil (5-Fu).

结果 Hela/MMC细胞对MMC有5.02倍耐药，对DDP有2.03倍交叉耐药，对VCR、ADM和5-Fu保持敏感；耐药细胞核分裂指数增加；耐药细胞内丝裂霉素蓄积量比亲代细胞低32.64%(P.01)。

Babies from 6 months on a cold, two days to start eating like a drug, and the second drug to do to eat for a week, the 3rd on the half-eaten cold cough on drugs, the 4th doctor started me playing吊针baby bottles from 2 to 9 times hanging bottle; final Cephalosporium吊针not used, azithromycin, penicillin;(disease good will Also a week before a cold cough) are now 13 months, or so over a week on the incidence, which I also go around the middle to open Chinese medicine to the baby food; sneezing, runny nose, cough, dyspnea, tonsil inflammation of the throat red swelling, sleep a sweat, they do not release,尿黄; I now do not dare to go the hospital, and also to the baby do not know what medicine he would eat better.

孩子从6个月就感冒，开始吃了2天药就好了，第二次吃了一星期药才好，第3次就感冒咳嗽吃半个月药，第4次医生就开始给我孩子打吊针了从2瓶一次次吊到9瓶；最后头孢吊针都没用了，用阿奇霉素，青霉素；（病好了也就过一星期又感冒咳嗽）现在都13个月大了，还是这样过一星期就发病，这中间我还到处去开中药给孩子吃；打喷嚏，流鼻水，咳嗽，气急，扁桃体发炎喉咙红肿，睡觉出大汗，便干放不出，尿黄；我现在都不敢去医院了，不知道还要给孩子吃什么药他才会好。

In the long-period toxicity test, DCG was administered repeatedly for 60 days, the dose were 72.6,36.3,18.2 g/kg respectively(It refered to the dose of material,which amounted to 52.6,26.3,13.2 times of 50 kg human clinic use). There is no other abnormal changes about color patem,action,urine and stool,but cibation, hydroposia, body weight and index part of hematological and hematobiochemical parameters,coefficient and histomorphological figure of organs were examined during administration phase.The rats were examined in 21 days after the cessation of DCG, the abnormal changes of index was completely recovered.

定喘颗粒大鼠给药60 d的毒性试验，剂量分别为72.6，36.3，18.2 g/kg（相当于临床50 kg人用量的52.6，26.3和13.2倍），给药期间各组大鼠毛色、活动、二便均无异常变化，但给药组大鼠摄食、饮水、体重以及部分血液学、血液生化学指标和脏器系数有异常变化，停药后，上述异常变化指标均已恢复正常。

HPLC was used to determine the concentrations of chlorogenic acid and baicalin in the plasma samples of rabbits,then analyze these data by the pharmacokinetics program 3P97 to determine the parameters of pharmacokinetics and the compartmental model of both ingredients in Yinhuang compound microenema.

采用高效液相色谱法测定黄芩苷和绿原酸在家兔体内的血药浓度，采用药动学程序3P97进行统计处理，确定药动学模型及相关药代动力学参数。

Life. But the intrinsic way of the Internet by wire confines accessing and using of the network information.

然而，Internet固有的有线连接方式大大限制了人们对网络信息的访问和使用。

I have a strong persuasion that this is true .

我坚信这是正确的。