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The ternary complexes of Ln with 8-hydroxyquinathroline and cinchophen (2-phenyl-quinoline-4-carboxylic, HL) had been synthesized and their composition, solubility, molar conductance, UV, IR, 1HNMR and XPS studied.

合成了3种辛可芬(2-苯基喹啉-4-甲酸,HL)与8-羟基喹啉的稀土三元配合物,通过元素分析,摩尔电导,UV,IR,1HNMR等的研究,确定配合物的化学组成为Ln3·H2OLn=Ce(Ⅲ,Nd,Sm,观察了它们的抗炎作用、半数致死量(LD50)和在霜剂中的配伍稳定性

In the present study,we intend to establish a new model of traumatic brain-steminjury,and to study the pathological changes of neuron,axon,astrocyte,plasmaproteins,blood vessel endothelia,synapse,neurotransmitter and cytokines in rat'sand human injured brain-stem,using routing histochemistry,immunohistochemistry,in situ hybridization,LSCM and computerized image analysis.

综合现有文献,本研究拟建立新的脑干损伤动物模型,并采用常规病理组化、免疫组化、原位杂交、LSCM以及计算机图像分析等技术,对动物及人体脑干组织中的神经元、星形胶质细胞、血浆蛋白及血管内皮细胞,以及轴索、突触、神经递质及细胞因子等进行研究,并对脑干损伤的致死性病变进行初步定位,以确立对脑干损伤的鉴定和推断脑干损伤时间有意义的客观指标。

Effects of gamma irradiation on shoot regeneration from leaf and bud growth in vitro of strawberry 1 The leaf was very sensitive to the gamma irradiation, and 'Toyonoka' was more sensitive than 'Akihime'. The LD50 (50% decrease of generation) of strawberry leaf was between 10 and 20 Gy. 2 Strawberry leaves were inoculated in vitro at 8 d-15 d after gamma rays irradiation, and the shoot regeneration rate from of 'Toyonoka' increased at 10 Gy and almost no difference from 20 Gy to 40 Gy. The positive effect dose for 'Akihime'.was from 10 Gy to 30 Gy, however, 40 Gy decreased shoot regeneration rate.

2.60Co γ辐射对不同品种草莓离体叶片再生及芽生长的影响 1)叶片对辐射敏感,叶片再生的半致死剂量为10 Gy-20 Gy,'丰香'品种比'章姬'品种敏感; 2)辐射后8 d-15 d接种,'丰香'叶片的不定芽再生率在剂量10 Gy呈上升趋势,而其他剂量则几乎未见有变化,'章姬'在剂量10 Gy-30 Gy,叶片的不定芽再生率提高较大,40 Gy仍表现轻微下降; 3)适宜的辐射剂量对草莓试管苗增殖有促进作用。

This work establishes a radical-based molecular mechanism whereby sublethal levels of antibiotics can lead to multidrug resistance, which has important implications for the widespread use and misuse of antibiotics.

这给我们构建了一个激进的分子机制,而抗生素的亚致死水平可以导致对多种药物的耐药,这对抗生素的广泛和滥用有重要的提示。

Methods①The animal model of braincontusion caused by free drop hammer was established.②The injuredtissue of rat brain were stained by TUNEL for apoptosis,immunohistochemistry for Caspase-3 and Feulgen"s for DNA. Image analysistechnique and the statistical method were employed to explorate thetemporal changes of injury time.③The DNA was extracted from ratcontusive tissue of brain and assayed by gel electrophoresis toinvestigate the relationship between the DNA fragmentation and injurytime.④One handred and seventeen cases of death from traumatic braininjury were retrospective researched to investigate the characteristicof TBI in forensic medicine.⑤The contusive tissue of human brain werestained by TUNEL, Caspase-3 immunohistochemistry and Feulgen"s methodsin the same way to analyze and disclose the linear relationship betweentemporal changes with injury time.

方法①建立大鼠自由落体打击脑损伤模型;②对不同损伤时间组的大鼠脑挫伤组织进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色,结合图像分析技术和统计学分析,探讨脑挫伤后神经细胞凋亡、DNA片段化和含量的时序性变化;③提取大鼠脑挫伤组织DNA进行琼脂糖凝胶电泳分析,观察DNA片段化随损伤时间的变化特点;④对117例颅脑损伤致死案例进行回顾性分析,探讨其法医学特点;⑤选择不同损伤时间的人脑挫伤组织同样进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色和分析,观察上述指标与损伤时间的关系。

Coalition members described the abuses as a 'silent holocaust' because the weapons deployed are so poorly publicised, and because individuals are dying as a result of deployments of directed energy weapons, and other deniable abuses of human rights that were also discussed during the conference.

联合的成员把这种虐待描述为一种&沉默的屠杀&,因为武器的配置很少被公开,并且受害者会被这种定向能武器致死,以及其他可否认的对人权的侵害,也在会议期间被讨论。

The preliminary localization of lethal lesions in in-jured brain-stem indicated that,lesions in the neural structure concerning the respira-tory center and cardiovascular center might be the cause of death,which were prin-cipally on or close to the line linking the posterior angle of fourth ventricle at thedorsal surface and interpeduncular fossa at the ventral side.

脑干损伤致死性病变的初步定位提示,第四脑室下角与脚间窝之间的连线及附近的神经结构受损可能是机体死亡的原因。根据文献,此部位与机体的呼吸中枢和心血管中枢有关。

Passive transfer of sera from mice immunized with expressed G1 and G2 conferred partial protection or prolongation of time to death from fatal hantaan virus infection in infant mice which challenged with hantaan virus at 10LD50. The spleen cells from mice immunized with G1, G2and NP can protect or prolongate the lifetime of infant mice infected with lethal dose (10LD50 or 40LD50) hantaan virus.

将重组G1、G2和NP免疫小鼠的血清和脾细胞注入汉滩病毒致死性感染的乳小鼠体内,发现G1、G2的免疫血清和G1、G2及NP免疫脾细胞对乳鼠有保护作用,同时在一组试验中观察到G1的脾细胞可显著缩短乳鼠的存活时间。

In this thesis, the acute lethal toxicity to Rana japonica tadpoles, the short-term phytotoxicity to Cucumis sativus and the population growth inhibition toxicity to Tetrahymena pyriformis of selected substituted phenols with multiple mechanisms of toxic action were compared and interspecies correlation was investigated.

本文比较了具有多种毒性作用机制的取代苯酚对两栖动物幼体的急性致死性毒性、对高等植物生长的抑制毒性以及对原生动物—四膜虫的种群生长抑制毒性,探讨了这一类污染物的毒性作用机制,进行了种间相关性研究。

Model of animals were established by putting rats in a container with slippy inner wall and half-full of water in it, letting them swimming freely till died of being drowned. Each rat from different groups was killed by breaking its neck except drowning group, then BW and SO_2 was detected, and its chest was cut open rapidly, lung was taken out and weight was measured accurately to count lung coefficient. Its lung appearance was observed and lung tissue was fixed with formalin, which was made into pathology slices with HE staining, and pathology change of the lung tissue was observed;Expression of IL-8 and IL-10 in lung tissue was detected with Immunocytochemistry, and correlation between them was analyzed .

动物模型制作采用将大鼠放入盛有黄河水的内壁光滑的容器内,让其自由泳动自然溺死,除溺水致死组外,各组动物分别在规定的时间点断头处死,行体重和血氧饱和度等指标检测,并在冰盘中迅速开胸取肺,准确称重以计算肺系数,观察各组大鼠肺脏外观,取肺组织福尔马林固定,制成病理切片HE染色,光镜下观察肺组织病理改变;用免疫组化的方法检测肺内IL-8、IL-10的表达,并对其相关性进行分析研究。

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