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The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明: 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h,胞质突起率和去核率没有显著的差异,突起率可高达85.4%,去核率达到100%; 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h,对去核效果没有显著影响; 3 对于体外成熟18~23 h的卵母细胞,随着成熟时间的延长,盲吸法的去核率降低,但没有影响秋水仙素诱导胞质突起的比率和去核率; 4 两种去核方法对重构胚的发育没有产生显著影响,但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

The mature embryo sac fertility, formation and development of embryo sac of 141 accessions of O. rufipogon Griff. collected from the six sampling sites in Gaozhou, Guangdong Province were studied by using the technology of whole-mount stain-clearing confocal laser scanning microscopy.

利用整体染色激光扫描共聚焦显微镜术,对采自广东省高州市6个地点共141个编号的高州普通野生稻的成熟胚囊育性和胚囊形成发育特点等进行研究。

The megagamete fertility, the quantity of the anther , pollen sac wall structure and the dehiscent state, the quantity of the pollen grain were observed in the flowering phase I

第Ⅰ花期观察供试材料的雌配子育性、颖花内花药数、花药吐露情况、成熟花粉囊壁的开裂状况与结构、花粉量

Malfunction will result in cystic fibrosis, chronic pancreatitis, male sterility, habitual constipation, keratoconjunctivitis sicca (KCS, also called dry eye syndrome). Over activation of CFTR is related to diarrhea, polycystic kidney disease.

该通道活动的异常与多种疾病的病理发生相关:CFTR氯离子通道功能丧失或活性降低可导致慢性胰腺炎、多囊肾病、男性不育、习惯性便秘和干眼病等;而功能的过度激活则可导致分泌性腹泻、多囊肾病等。

Panicle open, 10–30 cm; branches in distant whorls, capillary, flexuous, 5–15 cm, mostly unbranched, tipped by a raceme; racemes 2–5-noded with 7–13 spikelets, sometimes branched with up to 40 spikelets, disarticulating at maturity, one spikelet of a pair sessile, the other pedicellate; rachis internodes and pedicels slenderly clavate, margins ciliate, hairs 0.7–1 mm. Spikelets 4.5–5 mm; callus hairs ca. 1 mm; lower glume lanceolate, thinly pilose, veins smooth below middle, scaberulous above, apex acuminate; upper glume ciliate on margins, acuminate or emarginate and mucronate; lower floret sterile, palea reduced; upper lemma 2-lobed to middle; awn 5.5–8 mm.

圆锥花序打开,10-30厘米;在远轮生方面的分枝,发状,,5-15厘米,多数不分枝,被总状花序端部; 7-13小穗2-5有节具的总状花序,40小穗的有时分枝具可达,成熟时脱节,一对无梗,另一个的一小穗;棍棒状的轴节间和花梗slenderly,边缘具缘毛,头发0.7-1毫米小穗4.5-5毫米;胼胝体毛约1毫米;披针形,稀疏具柔毛的下部的颖片,脉平滑的中间以下,在上面微糙,先端渐尖;在边缘上具缘毛的上面颖片,渐尖的或微缺和短尖;不育的下部小花,退化的内稃;上面外稃2裂的至中部;芒5.5-8毫米花粉囊2-3毫米。

The dormant state in tardigrade (e.g ., tuns, cyst, resting eggs) can occur at any stage of life, which can help tardigrades survive some unfavorable environments, extending individual's life-span.

滞育包括包囊和休眠卵两种形式。缓步动物的三种休眠状态在其一生中任何一个阶段都能够出现,以度过极端不利的环境,并以此延长生物个体的寿命。

METHODS: The different combinations of the biofilm were put on the surface of chick embryo chorioallantoic membrane.

将制备的生物膜置于正对观察窗的鸡胚绒毛尿囊膜表面的血管最少处,然后贴在鸡胚绒毛尿囊膜表面上,封口膜封闭假气室,继续孵育。

Pinctada martensii ; pearl sac ; culture in vitro ; culture

马氏珠母贝;珍珠囊;体外培养;插囊育珠

She is good at treatment of the barrenness, the hysteromyoma, the endometriosis, the chronic pelvic inflammatory disease, the cervicitis, the salpingemphraxis, the ovulation failure,and so on, in the traditional Chinese medicalmethod. Especially she specializes in the Polycystic ovarian syndrome.

擅长运用祖国传统医学治疗男女不育不孕症、子宫肌瘤、子宫内膜异位症、慢性盆腔炎、宫颈炎、输卵管阻塞、排卵障碍等各种妇科疾病和男子精症、弱精症,并取得良好的疗效;尤其对治疗多囊卵囊综合征有独到见解。

METHODS: Constructing the CAM model by exposing CAM on the shell subpoint between 2 vitelline veins and generating an air chamber after 5 days of incubated with aseptic incubator at 37℃.

将鸡胚消毒后,置于37℃无菌恒温箱中孵育5 d,在距胚头1 cm两条卵黄静脉之间的卵壳投影部位磨切暴露尿囊膜,制成假气室,用无菌滤纸封闭窗口,制成鸡胚尿囊膜模型,备用。

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