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So, the abnormality of embryo sac was one of the factors that induce low seed setting percentage in autotetraploid rice.

从对13个品种的成熟胚囊育性调查的结果来看,同源四倍体水稻胚囊育性是影响其结实率的原因之一。

The fertility of mature embryo sac ranged from 57% to 92%, the mean was 76%.

成熟胚囊的育性在57%~92%之间,平均育性为76%。

The pollen and embryo sac fertility were controlled by two sets of different genes, and both of them made contributions to F〓 spikelet fertility.

花粉育性和胚囊育性分别出不同的遗传基因控制,两者共同决定小穗育性的高低。

Morphological and anatomical observationsshowed that the pol CMS plants in DGCMS-Yi-3A were complete sterile and hadno pollen sac differention at higher temperature condition,and were partialsterile and had 1-3 pollen sacs in each anther at lower temperature condition.

花器形态和花药发育的解剖观察表明,DGCMS-宜3A中表现波里马细胞质雄性不育的植株在高温条件下表现为彻底雄性不育,无花粉囊的分化;在低温条件下,表现部分雄性不育,可分化出1-3个花粉囊,产生少量可育花粉。

Morphological and anatomical observations showed that the pol CMS plants in RGCMS?117A or RGCMS S45A were complete male sterile ,had no pollen sac differentiation and had smaller petals and stamens at higher temperature situation.

花器形态和花药发育的解剖学观察表明,RGCMS S45A和RGCMS 117A中表现波里马细胞质雄性不育的植株,其花器形态和花药发育的解剖学特点与polCMS相同,即在高温条件下,花瓣较小,表现彻底不育,无花粉囊的分化;在低温条件下,花瓣变大,表现部分雄性不育,可分化出 1~ 4个花粉囊,并产生一定数量可育花粉。

The factors affecting the seed-set rate ofintersubspecific hybrids include embryo sac sterility,pollen sterility,anther dehiscence and non-synchronization of male and female sexual organsdevelopment in the same spikelet.

对水稻亚种间杂种及其稳定的多胚品系SB-1进行了细胞学、胚胎学和遗传学研究,主要结果如下:1、影响杂种低结实率的因素包括胚囊败育、花粉败育、花药不开裂和雌雄性器官发育进程不一致。

Microscopic observation on anther development process of cytoplasmic male sterile line"1815A"showed that its anther development was inhibited at the stage of archesporial cell differentiation.

以1815A不育系对应的保持系1815B作对照,对不育系进行细胞学观察,结果表明:随着花蕾的发育,败育的雄蕊一直处于孢原细胞时期,不能形成花粉囊并产生花粉母细胞,但雌蕊和维管束组织发育正常。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分,第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究,确立了一种较好的培养方法:与颗粒细胞共培养,找到了一种适合猪卵母细胞体外成熟的培养基:mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸,成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%,国外报道的最高成熟率为(85.7±4.1)%;第二部分对猪卵母细胞孤雌激活的化学方法进行了研究,确立了化学激活猪卵母细胞的两种最佳方法:1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min,再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中,卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min,再与8mM的DTT共孵育30min,卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%;第三部分对孤雌激活胚胎的培养条件进行了研究,确立了一种最佳的胚胎培养条件:在SOF简单培养基中添加颗粒细胞进行前3天的培养,然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养,其桑椹胚和囊胚的发育率为(59.5±3.2)%;第四部分研究了IGF-I

Selective abortion stage of the stamen was primarily manifested in the stage of archesportial cell formation through anatomic observation for male sterile flower and male fertile flower. There was no archesportial cell development or anther production and was a male sterile line without anther.

通过对不育系和可育系小花的细胞及形态解剖学观察,初步确定万寿菊雄蕊选择性败育的时期,为花药受阻于花药原基细胞分化期,无孢原细胞形成和药室分化,属于无花粉囊型不育系。

The treatment of high-hydroscopicity resin seed-coatingmaterial can fornl a water-stored ball of about 1. 8 cm × 1. 5 cm × 1. 2 cm around seed and root, the ball has the abilities of absorbing, keeping and providing water. As the ball containing pesticide, germicide and plant regulatorand releasing slowly, it may afford a suitable surroundings for germination an...

高吸水种衣剂包衣处理在种子周围及秧苗根部形成了18cm×1.5cm×1.2cm左右的&蓄水球囊&,不仅提高了种子出苗率、成秧率,有效地防止了多种病虫害及死苗的发生,壮大根群,提高了秧苗素质;而且实现了旱育秧苗根部带土抛植,提高了立亩速度和抗植伤能力,增加了旱育抛栽稻的产量。

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